J. Food Biochem. 44, e13137 (2020). HPTLC of kaempferol-3-O-rutinoside (1) and rutin (2) in Musa acuminata Colla on silica gel with ethyl acetate - toluene - formic acid - water 34:5:7:5. Detection by dipping into natural products reagent (0.5 % methanolic solution of 2-aminoethyl diphenylborinate), followed by dipping into a 5 % methanolic polyethylene glycol 400 solution. Qualitative identification under UV light at 366 nm. Radical scavenging activity was studied by dipping into a 0.2% methanolic DPPH* solution. The hRF values for (1) and (2) were 32 and 25, respectively. 

Front. Pharmacol. 12, 755941 (2021). High-throughput eight-dimensional (8D) hyphenation of normal-phase HPTLC with multi-imaging by ultraviolet, visible and fluorescence light detection as well as effect-directed assay and heart-cut of the bioactive zone to orthogonal reversed-phase high-performance liquid chromatography-photodiode array detection-heated electrospray ionization mass spectrometry. The method allowed the analysis of 68 powdered plant extracts (botanicals) which are added to food products in food industry and the study of antibacterials, estrogens, antiestrogens, androgens, and antiandrogens, as well as acetylcholinesterase, butyrylcholinesterase, α-amylase, α-glucosidase, β-glucosidase, β-glucuronidase, and tyrosinase inhibitors in an array of 1,292 profiles.

J. Agric. Food Chem. 69, 12686-12694 (2021). HPTLC of 2Z,8Z- and 2E,8Z-matricaria esters in European goldenrod (Solidago virgaurea) and E- and Z-dehydromatricaria esters in grass-leaved goldenrod (Solidago graminifolia) and showy goldenrod (Solidago speciosa) on silica gel with n-hexane − acetone 17:3. Detection by dipping into mycelium suspension of F. avenaceum and B. sorokiniana, followed by incubation in a vapor chamber at 21 °C for 48−72 h. The lack of visible white (F. avenaceum) or dark gray (B. sorokiniana) fungal mycelia indicated the inhibition zones on the bioautograms. Compounds were further analyzed by high-resolution mass spectrometry and nuclear magnetic resonance spectroscopy.

Journal Chromatogr A, 1641, 461727 (2021). HPTLc of an ethanolic maceration of Solidago gigantea roots (Asteraceae) on silica gel with n-hexane – isopropyl acetate – acetone 16:3:1, or n-hexane – isopropyl acetate – acetic acid 40:9:1. With the second mobile phase, acid residues had to be eliminated by 20 min automated drying or by 2 h incubation with potassium hydroxide in the opposite twin trough (followed by 15 min cold air streaming); this latter mobile phase allowed to obtain higher hRF values, but some butyrylcholinesterase (BChE) inhibiting activities were lost. The chromatograms were documented at UV 254 nm and 365 nm and white light before and after A) derivatization with vanillin – sulfuric acid reagent; B) enzymatic reaction by immersion into acetylcholinesterase, BChE, glucosidase and amylase solutions; C) Aliivibrio fischeri and Xanthomonas euvesicatoria bioassays, to detect activity against Gram-negative bacteria; D) Bacillus subtilis bioassay to detect activity against Gram-positive bacteria; E) a new antifungal assay with Fusarium avenaceum. For this assay, the chromatograms were immersed 6 s into the isolated mycelium suspension (diluted to OD600 0.4-0.8) and incubated in a vapor chamber at 21 °C for 48-72 h. Inhibition zones were indicated by the lack of visible white fungal hyphae. An aqueous solution of iodonitrotetrazolium (INT, 1 mg/ml) was sprayed on the plate to enhance the contrast (bright zones on a purple background). Benomyl (a benzimidazole fungicide) was used as positive control. Eight clerodane diterpenes (including kingidiol, hautriwaic lactone, and solidagoic acids A and B) were identified from six multipotent zones by bioassay-guided purification through preparative flash chromatography and HPLC, followed by HRMS and NMR, as well as by HPTLC hyphenated to quadrupole-orbitrap HRMS: A) by eluting with methanol (flow 100 µL/min) the compounds from the plate through the oval elution head of an interface of heated electro-spray ionization (spray voltage 3.5 kV, capillary temperature 270 °C, nitrogen as sheath and auxiliary gas, full scan in negative and positive ionization modes in m/z range 50-750); B) without eluent with a DART interface (Direct Analysis in Real-Time, needle voltage 4 kV, grid voltage 50 V, helium as gas, temperature 500 °C, full scan in positive ionization mode in m/z range 100-750).

J. Planar Chromatogr. 35, 189-195 (2022). Planar yeast estrogen screen (pYES) of estriol, daidzein, genistein, 17β-estradiol, 17α-ethinyl estradiol, estrone, 4-nonylphenol and bis(2-ethylhexyl) phthalate on silica gel with cyclohexane - methyl ethyl ketone 2:1 or cyclohexane - cyclopentyl-methyl ether 3:2. Detection under UV light at 272 nm.

J. Planar Chromatogr. 35, 139-151 (2022). HPTLC of diosgenin in Costus speciosus on silica gel with n-hexane - ethyl acetate 7:2. Detection by spraying with anisaldehyde  -sulfuric acid (0.5 mL anisaldehyde in 1 mL of 97 % sulfuric acid), followed by drying at 105 °C. Quantitative determination by absorbance measurement at 440 nm. The hRF value for diosgenin was 23. Linearity was between 0.1 and 0.7 µg/zone. The LOD and LOQ were 0.84 and 2.55 µg/zone. 

J. Planar Chromatogr. 35, 127-138 (2022). HPTLC of fresh fruit peels of some species of the genus Citrus L.: Citrus aurantium L. (bitter orange), C. unshiu Marc. (satsuma mandarin), C. reticulata Blanco (Robinson mandarin), C. sinensis L. (Washington Navel orange), C. limon Brum. (lemon), C. limetta (sweet lime), C. bergamia (bergamot), C. Medica (citron), C. paradisi Macf. (starruby grapefruit), C. maxima (pomelo) and C. maxima x C. paradisi (oroblanco) on silica gel with ethyl acetate - formic acid - acetic acid - water 100:11:11:26. Detection by dipping into a solution of NP (0.5% diphenylborinic acid aminoethyl ester in ethyl acetate), followed by dipping into a PEG (5 % PEG400 in dichloromethane). Tyrosinase inhibition analysis by dipping into 32 % ammonia, followed by drying and spraying with enzyme solution and incubation at room temperature for 10 min. DPPH radical scavenging activity by dipping into a solution of 0.05 % DPPH in methanol, followed by incubation for 30 min in darkness. 

J. Planar Chromatogr. 35, 103-116 (2022). HPTLC of Daqinjiao standard decoction containing the following herbs: Gentianae Macrophyllae Radix (1), Paeoniae Radix Alba (2), Glycyrrhizae Radix et Rhizoma (3), Notopterygii Rhizoma et Radix (4), Saposhnikoviae Radix (5), Angelicae Pubescentis Radix (6), Angelicae Sinensis Radix and Chuanxiong Rhizoma (7), Scutellariae Radix (8) on silica gel with ethyl acetate - methanol - water 15:2:1 for (1) to (5), n-hexane - ethyl acetate 7:3 for (6) and (7) and toluene - ethyl acetate - methanol - formic acid 15:5:1.5:3 for (8). Detection by spraying with 5 % solution of vanillin in sulfuric acid, followed by heating at 105 °C for 5 min for (1) to (3), (5) and (6) and spraying with 2 % iron trichloride for (8). Qualitative identification under UV light at 254 and 366 nm. Reference standards were gentiopicroside for (1), paeoniflorin for (2), liquiritin for (3), nodakenin for (4), 5-O-methylvisammioside for (5), osthole for (6), ligustilide for (7) and baicalein and wogonin for (8). The hRF values for reference standards for (1) to (8) were 40, 35, 46, 28, 24, 37, 65 and 56, respectively.