Chinese J. Pharm. Anal. (3), 547-553 (2014). Rhizoma Cimicifugae is a medicinal herb and one of the main ingredients of TCM preparations for the treatment of measles, sore mouth and throat, rashes, chronic diarrhea etc. For quality control, TLC of the extracts of the drug sample on silica gel with the lower phase of chloroform – methanol – water below 10 °C, detection under UV 366 nm revealed 9 pale blue zones. Detection by spraying with a freshly prepared solution of vanillin – sulfuric acid – acetic acid 1:10:1000 followed by heating at 105 ˚C until the zones are visible under UV 366 nm, this detection mode revealed 16 zones of different color. The resulting images were converted to digital profiles to carry out similarity and two-dimensional clustering analysis. The main components were identified using QTofMS with cimifugin, prim-O-glucosylcimifugin, 27-deoxyactein, and ferulic acid as references. Based on the fingerprints the genuine drugs were easily differentiated from adulterant drugs.

Planta Medica 82, 11/12, 1051-1057 (2016). The crude ethanol extract of the aerial parts of the liverwort Chiloscyphus polyanthos, its fractions and seven pungent sesquiterpene lactones isolated therefrom were analyzed against Candida albicans strains (hypersensitive mutant DSY2621, wild-type parent CAF2-1). 20 mL of C. albicans suspension were spread and solidified on the plate charged with samples (20 µg/spot), blank (methanol) or reference (miconazole); without any development, the plate was incubated overnight at 37 °C, sprayed with methyl thiazolyl tetrazolium chloride (MTT 0.25 %) and incubated for 6 h at 37 °C to obtain a purple background. Diplophyllolide A was active on both strains, four other lactones only on DSY2621 (l-diplophyllin, dihydrodiplophyllin, ent-3-oxodiplophyllin, 3β-hydroxyeudesma-4,11-dien-12,8α-olide)._x000D_

J. Liq. Chromatogr. Relat. Technol. 39, 264-270 (2016). HPTLC of estradiol hemihydrate on silica gel (1) or RP-18 (2) with dichloromethane – ethyl acetate 4:1 for (1) and methanol – water 9:1 for (2). Quantitative determination by absorbance measurement at 200 nm. The LOD was 239 ng/zone using (1) and 93 ng/zone using (2).

J. Chromatogr. Sci. 52 (6) 559-565 (2014). TLC of sertindole subjected to stress stability studies, including acid, alkali, oxidative, photolytic and thermal degradation, on silica gel with methanol – ethyl acetate – 33 % ammonia 10:90:1, detection under UV 254 nm (the hRf was 71 for sertindole and 16 for its oxidative degradation product), identification by IR and GC/MS, quantification by densitometry at 227 nm with a linearity range of the calibration curve from 2 to 14 µg/band. The intermediate precisions and repeatabilities were < 2%. The method is suitable for quality control laboratories as stability-indicating method and for routine analysis without any preliminary separation step.

Phytochemistry 134, 6-25 (2016). The review describes the chemistry of Dillenia species, including TLC methodologies for the analysis of flavonoids, triterpenoids and miscellaneous compounds, as well as TLC bioautography for the identification of betulinic acid and dillenic acid and their activity against E. coli and B. subtilis.

growing in Nilgiri hills of India. J. Planar Chromatogr. 29, 347-355 (2016). HPTLC of forskolin in the roots of Coleus forskohlii on silica gel with toluene – ethyl acetate – methanol 180:60:1. Detection by dipping into anisaldehyde ‒ sulfuric acid reagent. Quantitative determination by absorbance measurement at 545 nm. The hRF value for forskolin was 48. Linearity was between 20 and 100 ng/zone. The intermediate precisions were below 1.6 % (n=3). The LOD and LOQ were 1 and 3 ng/zone, respectively. Recoveries were between 98.3 and 101.5 %.

J. Planar Chromatogr. 29, 372-379 (2016). HPTLC of vitamin E (1) and vinpocetine (2) in its alkaline-induced degradation product (3) on silica gel with methanol – chloroform – ethyl acetate – glacial acetic acid – ammonia 60:20:20:5:1. Quantitative determination by absorbance measurement at 235 nm. The hRF values for (1) to (3) were 81, 62 and 41, respectively. Linearities were between 0.2-2 µg/zone for (1), 0.1-1.5 µg/zone for (2) and 0.1-1 µg/zone for (3). The intermediate precisions were below 0.7 % (n=3). The LODs and LOQs were 67 and 198 ng/zone for (1), 32 and 98 ng/zone for (2) and 29 and 87 ng/zone for (3). Average recoveries were 99.6 % for (1), 100.0 % for (2) and 99.9 % for (3). The results were statistically compared with those obtained by a HPLC method and no significant differences were found.

J. Ethnopharmacol. 203, 127-162 (2017). Comprehensive review of the genus Peganum, including phytochemistry and analytical methods such as TLC, TLC-bioautography and HPTLC for the determination of analytes such as harmaline, harmine, vasicine and vasicinone.