Chinese J. of Lishizhen Trad. Med. & Pharm. 23 (5), 1180-1181 (2012). Elaeagnus gonyanthes Benth. is a medicinal herb which grows in southern China. The crude drug is used in folk medicine for treating asthma, chronic bronchitis, arthritis, low back pain, traumatic swelling, etc. For identification, TLC of the extracts of different plant parts (leaf, stem) and the standards oleanic acid and ursolic acid on silica gel with cyclohexane – chloroform – ethyl acetate – glacial acetic acid 200:50:80:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight. Quantification of oleanic acid and ursolic acid by HPLC.

J. of China Pharm. 22 (1), 20-22 (2014). Ganmaoting Peifang granule is a herbal TCM for the treatment of cold, influenza, cough, sore throat, etc. For quality control, TLC on silica gel 1) for Fructus Arctii, with chloroform – methanol – water 40:8:1, detection by spraying with 10 % sulfuric acid in ethanol and heating mildly, viewing in daylight; 2) for Radix Isatidis, with n-butanol – glacial acetic acid – water 19:5:5, detection by spraying with 0.1 % ninhydrin in propanone and heating at 105 °C, viewing in daylight. Quantification of arctiin by HPLC.

J. Planar Chromatogr. 26, 336-342 (2013). HPTLC of diosmin (1), hesperidin (2), and ascorbic acid (3) on silica gel with ethyl acetate - methanol - water 15:3:2 for (1) and (2) and ethyl acetate - methanol - water - acetic acid 15:10:2:1 for (3). Quantification by absorbance measurement at 340 nm for (1), 286 nm for (2) and 265 nm for (3). The hRf values for (1), (2) and (3) were 34, 40 and 56, respectively. Linearity was in the range of 100-800 ng/zone for (1) and (2) and 50-400 ng/zone for (3). LOD and LOQ were 6 and 17 ng/zone for (1), 4 and 13 ng/zone for (2) and 4 and 13 ng/zone for (3), respectively. Recoveries were in the range of 98.1-99.3 % for (1), 98.4-99.5 % for (2) and 98.0-99.1 % for (3). Intermediate/interday/intra-day precision was below 2 % (n=6).

J. Chil. Chem. Soc. 58, 1663-1666 (2013). HPTLC of rosiglitazone (1) and glimepiride (2) in tablet dosage form on silica gel with methanol - toluene - ethyl acetate 1:8:1. Quantitative determination by absorbance measurement at 228 nm. The hRf values for (1) and (2) were 39 and 20, respectively. Linearity was in the range of 100-1500 ng/zone for (1) and 100-1500 ng/zone for (2). LOD and LOQ were 30 and 35 ng/zone for (1) and 85 and 90 ng/zone for (2), respectively. Recovery was 96 % for (1) and (2). Intermediate intra- and inter-day precision was below 2 % (n=6).

J. of China Pharm. 21 (14), 41-43 (2013). Jinmaxing Zhikepian tablets are a herbal TCM preparation for the treatment of asthma, and acute and chronic bronchitis. For quality control, TLC on silica gel 1) for Peucedanum praeruptorum Dunn, Scutellaria baicalensis and Herbal Ephedrae, with chloroform – methanol – ammonia 40:8:1, detection a) under UV 366 nm and 254 nm, b) by spraying with 2 % ninhydrin in ethanol and heating at 100 °C until the zones are visible in daylight; 2) for Lonicera Japonica, with the upper phase of ethyl acetate – formic acid – water 3:1:1, detection by heating at 100 °C for 5 min and viewing under UV 254 nm; 3) Eriobotrya japonica Thunb., with cyclohexane – ethyl acetate – formic acid 150:40:3, detection by spraying with 10 % sulfuric acid in ethanol and heating at 100 °C until the zones are visible in daylight; 4) for Semen Armeniacae Amarum, with cyclohexane – ethyl acetate – formic acid 150:20:1, detection by spraying with 2 % phosphomolybdic acid in ethanol and heating at 100 °C until the zones are visible in daylight; 5) for Platycodon grandiflorus, with chloroform – ethyl acetate – methanol – ammonia 4:1:4:1, detection by spraying with 5 % ninhydrin in sulfuric acid – ethanol 1:6 and heating until the zones are visible in daylight. Quantification of pseudoephedrine hydrochloride, ephedrine hydrochloride and vitamine B17 by HPLC.

in Shanhaidanpian tablets) (Chinese). J. of China Pharm. 26 (9), 997-999 (2012). Shanhaidanpian tablets are a herbal TCM preparation for the treatment of poor blood flow, blood stasis and chest pain syndrome. For quality control, TLC on silica gel with toluene – ethyl acetate – formic acid 20:2:1, detection by exposure to vapors until the zones are visible in daylight. Quantitative determination of 2,3,5,4’-tetrahydroxystilbene-2-O-beta-D-glucoside by HPLC.

J. Planar Chromatogr. 26, 427-434 (2013). HPTLC of oseltamivir (1) in pharmaceuticals counterfeited with ascorbic acid (2) on silica gel with methanol - water 3:2 +1 drop ammonia. Quantitative determination by absorbance measurement at 254 nm. The hRf values for (1) and (2) were 70 and 83, respectively. Linearity was between 5 and 14 µg/zone. LOD and LOQ were 2 and 5 µg/zone. Average recovery (by standard addition) was found to be 100.6 %. Intermediate intra- and inter-day precision was below 1.6 %. Comparing with colorimetric method, HPTLC method provided advantages in terms of speed, lower cost, and environmental protection without sacrificing accuracy.

J. Liq. Chromatogr. Relat. Technol. 37, 2814-2828 (2014). HPTLC of arylureas and arylacetamides derivatives on RP-18 with 1-13 % water in dimethylsulfoxide (increment 3 %). Detection under UV 254 nm. A quantitative structure-retention relationship study allowed to investigate the retention behavior of these substances as well as determination of lipophilicity related to the biological activity towards dopamine D2 and 5-hydroxy-tryptamine receptors.