Chinese J. Hosp. Pharm. (Zhongguo Yiyuan yaoxue Zazhi) 25 (1), 90-92 (2005). TLC on silica gel with chloroform - ethyl acetate - formic acid 2:2:1. Detection under UV light. Identification by fingerprint technique. Quantification by densitometry at 325 nm. Validation of the method by investigation of linearity (0.52 µg - 4.68 µg, r = 0.9992); precision (RSD = 2.20 %, n = 5); reproducibility of five time assay towards the same sample (RSD = 3.10 %); standard addition recovery (99.0 %, RSD = 0.26 %, n = 5). The results for three real life samples are given.

Part II. J. Chromatogr. A 1007 (1-2), 157-164 (2003). Quantitative structure–activity relationship (QSAR) analysis of H1-antihistamine activity was carried out and chromatographic data of 2-[2-(phenylamino)thiazol-4-yl]ethanamine, 2-(2-benzyl-4-thiazolyl)ethanamine, 2-(2-benzhydrylthiazol-4-yl)ethylamine derivative, and 2-(1-piperazinyl- and 2-(hexahydro-1H-1,4-diazepin-1-yl)benzothiazole derivatives were obtained. Silica gel impregnated with solutions of selected amino acid mixtures (-Asp, Asn, -Thr and -Lys) were used in two developing solvents as human histamine H1-receptor (hH1R) antagonistic interaction models. Lipophilicity data of the examined compounds were obtained and used in the QSAR assay. Using regression analysis, relationships between chromatographic and biological activity data were found. The correlations obtained in the present experiment with NP-TLC are more significant that those obtained in the experiment with RP2-TLC because of the optimal fitting of the chromatographic system conditions to the lipophilicity of solutes. All proposed chromatographic models should facilitate pre-selection of the new drug candidates. The correlations of calculated pA2(H1) values of the tested compounds predicted by the use of the best equations versus their pA2(H1) obtained from the biological tests were significant (R2= 0.91-0.94).

Abstract G-28, IPC (2005). HPTLC of rosuvastatin on silica gel with chloroform - methanol - toluene 3:1:1. Quantitative determination by absorbance measurement. The hRf value of rosuvastatin was 53, recovery rate was between 98-102 %, LOD was 8 ng/spot and LOQ 26 ng/spot.

Abstract GP-18, IPC (2005). HPTLC of nimesulide and chlorzoxazone in tablets on silica gel (prewashed with methanol) with toluene - acetone - ammonia 50:50:4. Paracetamol was used as internal standard. Quantitative determination by absorbance measurement at 265 nm. Nimesulide, chlorzoxazone and paracetamol showed hRf values of 80, 73 and 42, respectively. Linearity was obtained between 0.2-1.0 mg/mL with recovery rates of 99.6-100.3 % for both compounds.The method was validated for accuracy, precision, linearity, LOD, and LOQ.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27 (4), 407-410 (2005). TLC of the extracts on silica gel with 1) benzene - ethanol 2:1 and 4:1; 2) n-butanol - glacial acetic acid - water 7:1:2; 3) chloroform - methanol 9:1. Detection under UV 365 and 254 nm. Identification by fingerprint technique. Quantification of 2, 3, 5, 4’-tetrahydroxystilbene-2-O-beta-D-glucoside by HPLC. The analysis results for a group of real life samples are given.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27 (7), 786-790 (2005). TLC of Hainan holly on silica gel with 1) ethyl acetate - butanone - formic acid - water 5:3:1:1; 2) ethyl acetate - formic acid - water 14:5:5; 3) benzene - acetone - methanol - formic acid 85:15:10:2. Detection 1) by spraying with 1 % AlCl3 solution; 2) under UV 365 nm; 3) by spraying with 5 % phosphomolybdic acid in ethanol followed by heating at 105 ºC until the spots are visualized. Identification by fingerprint techniques. Quantification of rutin by HPLC. Discussion of the optimum processing procedure by comparison of TLC fingerprints and HPLC results of rutin content.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27 (7), 854-856 (2005). TLC of chenodeoxycholic acid in Hedan tablets on silica gel with n-hexane - ethyl acetate - acetic acid - methanol 20:25:2:3. Detection by spraying with 10 % H2SO4 in ethanol followed by heating at 105 ºC until the spots are visualized. Quantification of chenodeoxycholic acid by densitometry at 375 nm. Validation of the procedure by investigation of its linearity range (0.47 - 2.33 µg/spot, R = 0.9992); of its repeatability (3.3 %, n = 5); of its precision (3.9 %, n = 5 within plate and 4.7 % n= 5 plate-to-plate); and its standard addition recovery (98.4%, RSD = 2.5 %, n = 5).

Indian J. Pharm Sciences 67 (6), 721-726 (2005). HPTLC of gallic acid in ethanolic extracts of rhizomes from curculigo orchioides on silica gel with toluene - ethyl acetate - acetic acid 25:15:1. Quantitative determination by absorbance measurement at 260 nm. The method was validated according to ICH guidelines. Rhizomes and their marketed formulation were found to contain 2.5 % and 5.0 % of gallic acid.