J. of China Pharm. 23 (5), 29-31 (2014). Xiaoke Maikang Keli granule is a herbal TCM for treatment of diabetes and peripheral neuropathy etc. For quality control, TLC for (1) Achyranthes bidentata, on silica gel with chloroform – methanol 40:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC until the zones are clearly visualized, evaluation under white light; (2) Salvia miltiorrhiza, on silica gel with chloroform – acetone – formic acid 79:11:10, detection under UV 254 nm; (3) for Fructus Lycii, on silica gel with ethyl acetate – chloroform – formic acid 5:4:2, detection under UV 366 nm; (4) for Taxillus sutchuenensis, on silica gel with water-saturated toluene – ethyl formate – formic acid 5:3:1, detection by spraying with 5 % aluminum trichloride in ethanol, evaluation under UV 366 nm; (5) for Corydalis yanhusuo, on silica gel with petroleum ether (60-90 ºC) – ethyl acetate 1:1, detection by exposure to iodine vapors for a few seconds and evaluation under UV 366 nm; (5) for Astragalus membranaceus, on silica gel with chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible, evaluation under UV 366 nm.

J. Liq. Chromatogr. Relat. Technol. 40, 320-326 (2017). HPTLC of 31 substances of toxicological interest on RP-18 with 60 % acetonitrile in buffer pH 6.0, on silica gel with chloroform – methanol 9:1 and using pressurized planar electrochromatography (PPEC) on RP-18 with 60 % acetonitrile in buffer pH 2.2. The authors showed the application of the migration distance of the solutes, obtained by the PPEC technique, with a proposed equation allowed for increase of likely identification of substances in toxicological analysis.

Planta Medica 82(18), 1568-1575 (2016). The flash chromatography fractionation of an enriched hydro-methanolic extract of Maesa argentea leaves was monitored through TLC on silica gel with n-butanol – acetic acid – water 4:1:5. Saponins (including four new antiparasitic triterpenoids, maesargentosides I-IV) were detected with anisaldehyde sulfuric acid reagent.

Microbiol. Res. 199, 89-97 (2017). HPTLC bioautography of lipopeptide crude extracts from wild-type strain Bacillus subtilis 9407 on silica gel with chloroform – methanol – water 65:25:4. The plates were laid on the surface of a potato dextrose agar containing fungal spores at room temperature for 2 h and then removed. Incubation at 28 °C for 5 days. The hRF value for fengycin was 15.

J. Ethnopharmacol. 197, 165-172 (2017). HPTLC of arjunolic acid (1), arjunetin (2), berberin (3), piperine (4), resveratrol (5) and withaferin-A (6) in Ayurvedic formulation on silica gel with ethyl acetate – toluene – formic acid – acetic acid 6:3:5:1 for (1), n-butanol – glacial acetic acid – water 12:3:4 for (2), toluene – ethyl acetate – diethylether 6:3:1 for (3), chloroform – ethyl acetate – formic acid 25:10:1 for (4) and chloroform – methanol 19:1 for (5) and (6). Detection of (1-4) by spraying with anisaldehyde sulfuric acid reagent. Quantitative determination by absorbance measurement at 690 nm for (1) and (2), 366 nm for (3), 330 nm for (4), 307 nm for (5) and 225 nm for (5) and (6). The hRF values for (1-6) were 67, 31, 58, 24, 32 and 37, respectively.

J. Planar Chromatogr. 30, 411-417 (2017). HPTLC of paraben solutions and a complex matrix such as honey spiked with 5-hydroxymethylfurfural to study the influence of different application parameters on quantitative analysis. The investigated parameters included application geometries (spot, band and area) and their respective application modes (contact and spray-on), various dosage speeds, different band lengths, overspotted application versus application of mixture solutions as well as influence of the dosing volume on the result. These parameters have influence on method development and validation results.

J. Planar Chromatogr. 30, 423-426 (2017). 2D-HPTLC of phytoestrogenic active compounds in the root of Glycyrrhiza glabra on RP-18 with hexane – ethyl acetate – acetone 9:3:2 in the first direction and acetone – water 3:2 in the second direction. Effect-direct analysis by dipping into a yeast suspension followed by incubation at 30 °C for 4 h, drying at 37 °C for 15 min and spraying with the combined reaction buffer C (20 mL reaction buffer C is mixed with 0.2 mL of a freshly prepared solution of 0.05 g/mL 4-methylumbelliferyl-ß-D-galactopyranoside in DMSO or 0.2 mL of a freshly prepared solution of 0.05 g/mL 5-bromo-4-chloro-3-indoxyl-ß-D-galactopyranoside (X-Gal) in DMSO). Fluorescence detection at UV 366 nm._x000D_

J. Planar Chromatogr. 30, 350-356 (2017). HPTLC for the enantioresolution of atenolol, betaxolol, and orciprenaline racemic mixtures using the chiral selector (S)-Glu as an additive of the stationary phase (A) or the mobile phase (B). For method (A), the chiral selector (50 mL of 0.5 % (S)-Glu) was mixed with a slurry of silica gel (25 g) and the plate was developed with acetonitrile – methanol – dichloromethane – water 10:3:3:2. For method (B), the mobile phase was acetonitrile – methanol – water 7:1:1 admixed with 0.5 % (S)-Glu. Detection by exposure to iodine vapor. LOD for each enantiomer was in the range of 1.4-1.9 μg/zone. Recovery was between 96.2-99.1 % for_x000D_
both (S)- and (R)-atenolol.