Phytochem. Anal. 20, 511-515 (2009). Bioautography of alpha-D-glucosidase (1) and beta-D-glucosidase (2) in buffer solution (sodium acetate 4.1 % in water pH=7.5) sprayed onto a silica gel plate. Incubation at room temperature for 60 min for (1) and at 37 °C for 20 min for (2). For detection of the active enzyme, solutions of 2-naphthyl-alpha-D-glucopyranoside or 2-naphthyl-beta-D-glucopyranoside and Fast Blue salt were mixed at a ratio of 1:1 for (1) or 1:4 for (2), and sprayed onto the plate to give a purple background coloration after 2-5 min. Methanol extracts of the aerial parts of Tussilago farfara and Urtica dioica were tested as enzyme inhibitors and visualized as white spots on the TLC plates.

Photoreactivity of biologically active compounds. Pharmazie 64, 428-435 (2009). TLC of riboflavin and lumichrome (7,8-dimethylbenzo[g]pteridine-2,4-(1H,3H)-dione) on silica gel with acetic acid - acetone - methanol - benzene 1:1:4:14 Detection under visible light and UV 254 and 366 nm.

The Pharma Review 7(39), 151-153 (2009). HPTLC of atorvastatin calcium and fenofibrate on silica gel (pre-washed with methanol) with chloroform - methanol 4:1 over 20 mm with chamber saturation. The hRf value of atorvastatin calcium was 29 and of fenofibrate 77. The method was linear in the range of 200-1000 ng/band for atorvastatin calcium and 320-1600 ng/band for fenofibrate.

Chinese J. Hospit. Pharm. 29 (8), 686-688 (2009). TLC of the extracts of Yixuean pills on silica gel with 1) chloroform - ethyl acetate - acetone - formic acid 60:25:25:4; 2) n-hexane - chloroform - methanol 15:5:2; 3) ethyl acetate - formic acid - acetic acid - water 15:1:1:2. Detection 1) under UV 254 nm; 2) by exposure to iodine vapor and under UV 254 nm; 3) by spraying with 10 % sulfuric acid in ethanol followed by heating at 105 °C until coloration evaluation under visible light and UV 254 nm.

J. Planar Chromatogr. 22, 349-354 (2009). HPTLC of flurbiprofen (2-(3-fluoro-4-phenyl)phenylpropanoic acid) and degradation products on silica gel, prewashed with methanol, with chloroform - acetone - xylene 5:2:1 in a twin trough chamber saturated for 20 min. Quantitative determination by absorbance measurement (the authors report no wavelength). Linearity was between 50 and 600 ng/band. The limit of detection and quantification was 10 and 32 ng/band, respectively.

J. Planar Chromatogr. 22, 405-410 (2009). TLC of hydrochlorothiazide, triamterene, furosemide, and spironolactone on silica gel with hexane - ethyl acetate - methanol - water - acetic acid 42:40:15:2:1 with chamber saturation. Quantitative determination by absorbance measurement at 264 nm. The limit of detection for the different compounds was between 22 and 150 ng/band, and the limit of quantification was between 68 and 450 ng/band.

60th Indian Pharmaceutical Congress PA-213 (2008). HPTLC of atorvastatin calcium and amlodipine besilate on silica gel with chloroform - toluene - methanol - water 55:10:20:2. Quantitative determination by absorbance measurement at 242 nm. The calibration curve was found to be linear between 400 and 1200 ng/spot for both atorvastatin calcium and amlodipine besilate. The limit of detection and the limit of quantification for atorvastatin calcium were 100 and 400 ng/spot, and for amlodipine besilate 200 and 400 ng/spot, respectively.

Abstract No. F-263, 61st IPC (2009). HPTLC of repaglinide (REPA) and rosiglitazone (ROSI) on silica gel with benzene - methanol - acetone - acetic acid 80:10:9:1. Quantitative determination by absorbance measurement at 266 nm. Lienarity was in the range of 800-2800 ng/band and 400-2400 ng/band for REPA and ROSI, respectively. Recovery was 101.7 and 99.5 % for REPA and ROSI, respectively.