J. Chromatogr. B 848 (2), 232-238 (2007). HPTLC of boswellic acids in Boswellia serrata extract and 11-keto-beta-boswellic acid in human plasma on silica gel with hexane - chloroform - methanol 10:10:1. Quantitative determination by absorbance measurement at 260 nm. The linearity range for 11-keto-beta-boswellic acid spiked in 1 mL of plasma was 29 - 146 ng/spot, with a recovery of 91.7 %. The limit of detection and quantification for 11-keto-beta-boswellic acid in human plasma was 9 and 29 ng/mL, respectively. The method was applied for the determination of 11-keto-beta-boswellic acid plasma levels in a clinical pilot study.

Rapid Commun. Mass Spectrom. 23, 2597-2604 (2009). RP-TLC of pharmaceutical formulations containing paracetamol, ephedrine, codeine, and caffeine on hydrocarbon-impregnated silica with methanol - water 1:1. Detection by desorption electrospray ionization (DESI) combined with ion mobility mass spectrometry (IM-MS). The limit of detection was 9, 16, 34, 239 and 225 µg/cm2 for codeine, caffeine, ephedrine and paracetamol, respectively.

Acta Chromatographica 20 (4), 637-642 (2008). TLC of thiamine hydrochloride from riboflavin, nicotinic acid, calcium D-pantothenate, pyridoxine hydrochloride, cyanocobalamin, and ascorbic acid on silica gel with dioxane - water 1:1. Detection under UV light. Examination of the effect of impurities (metal cations and inorganic anions) on the chromatography of thiamine hydrochloride. The detection limit for thiamine hydrochloride was 90 ng/spot and %RSD of thiamine hydrochloride was 14.9 % (n=5).

Ind. J. Pharma. Sci. 8(4), 198-201(2009). HPTLC of nebivolol hydrochloride and valsartan on silica gel with ethyl acetate - methanol - acetic acid 12:2:1 in a twin trough chamber saturated for 15 min. Quantitative determination by absorbance measurement at 240 nm for valsartan and 280 nm for nebivolol hydrochloride. The method was found to be linear in the range of 600-1400 ng/band for valsartan 1200-2800 ng/band for nebivolol. The sample were subjected to different stress conditions (acid, alkali, oxidation, photolysis, thermal) and all degradation products were well separated from the main compounds.

J. Chinese Trad. & Herb. Drugs 40 (8), 1249-1252 (2009). TLC of extracts of the TCM drug on silica gel with 1) methanol - acetic acid - water 18:1:4; 2) petroleum ether (60-90 °C) - ethyl acetate 1:1; 3) toluene - ethyl acetate - methanol 5:5:3; 4) petroleum ether (60-90 °C) - ethyl acetate - formic acid 15:5:1. Detection 1) by spraying with potassium iodobismuthate reagent; 2) under UV 254 nm; 3) by spraying with 5 % AlCl3 in ethanol and evaluation under UV 365 nm.

Abstract No. F-269, 61st IPC (2009). HPTLC of hydrochlorothiazide and irbesartan on silica gel with acetonitrile - chloroform 5:6. The hRf value was 27 and 45 for irbesartan and hydrochlorothiazide, respectively. Quantitative determination by absorbance measurement at 270 nm. The sample was exposed to different stress conditions (acid, alkali, oxidative, photodegradation, thermal). Neither of the compounds showed degradation under thermal and photodegradation conditions, but both compounds showed significant degradation under acid, alkali and hydrolytic conditions. Degraded products were well resolved from the parent compounds.

Abstract No. F-284, 61st IPC (2009). HPTLC of diclofenac sodium and thiocolchicoside on silica gel with toluene - ethyl acetate - methanol 5:3:2. The hRf value was 17 and 53 for thiocolchicoside and diclofenac sodium, respectively. Quantitative determination by absorbance measurement at 285 nm. The method was linear in the range of 50-300 ng/band for both drugs. Recovery was 100.5-101.1 %.

Abstract No. F-251, 61st IPC (2009). HPTLC of doxazosin mesylate on silica gel with acetone - toluene - 25 % ammonia 60:40:1. The hRf value was 65. Quantitative determination by absorbance measurement at 251 nm. Linearity was in the range of 20-100 ng/band. Recovery was 103.3 %.