J. of China Pharm. 22 (17), 32-33 (2013). Guiqi Tongru Heji compound oral liquid is a herbal TCM preparation for the treatment of postpartum hypogalactia. For quality control, TLC on silica gel 1) for Vaccaria segetalis, with chloroform – methanol – water 15:7:2, detection by spraying with fresh solution of (bismuth subnitrate - glacial acetic acid – water 17:200:800) – 40 % potassium iodate – glacial acetic acid – water 1:1:4:15, evaluation in daylight; 2) for Scrophularia ningpoensis Hemsl., with ethyl acetate - methanol – formic acid – glacial acetic acid - water 30:1:2:2:4, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones were visible in daylight; 3) for Angelica sinensis, with n-hexane – ethyl acetate 4:1, detection under UV 366 nm.

J. Ethnopharmacol. 156, 309-315 (2014). HPTLC of (1) phyllanthin and (2) ellagic acid in the whole plant of Phyllanthus rheedei on silica gel with toluene - ethyl acetate - formic acid 6:2:1, detection at UV 366 nm. The hRF values of (1) and (2) were 47 and 27, respectively. Fractions rich in phyllanthin and ellagic acid showed anti-HBV and hepatoprotective activity.

lanceolata with an energy audit (thermal × microwave × acoustic):_x000D_ a case study of HPTLC determination with additional specificity using on-line/off-line_x000D_ coupling with DAD/NIR/ESI-MS. Phytochem. Anal. 25, 551-560 (2014). HPTLC of (1)taraxasterol, (2) taraxasterol acetate and (3) stigmasterol in P. lanceolata on silica gel with hexane - ethyl acetate 22:3, detection by spraying with anisaldehyde sulphuric acid reagent, followed by air drying for 40 min. Quantitative determination by absorbance measurement at 645 nm. The hRF values of (1) to (3) were 75, 33 and 19. Linearity was in the range of 1-5 μg/zone for (1) to (3). The intermediate intra-day and inter-day precisions were below 2.3 % (n=3). The LOD and LOQ were 530 and 1760 ng/zone for (1), 240 and 790 ng/zone for (2) and 110 and 370 ng/zone for (3), respectively. Recoveries were in the range of 98-105 % for (1), 101-131 % for (2) and 95-116 % for (3).

J. Liq. Chrom. Rel. Tech. 38, 521-531 (2015). HPTLC of pitavastatin calcium on silica gel with ethyl acetate – methanol – toluene – glacial acetic acid 40:10:50:1. Quantitative determination by absorbance measurement at 335 nm. The hRF value of pitavastatin was 40. Linearity was between 25 and 150 ng/zone. The LOD and LOQ were 5 and 14 ng/zone. The intermediate intra-day and inter-day precisions were below 2.9 % (n=3). Recoveries were found in the range of 100.2-102.4 %.

J. China Pharm. Univ. 22 (23), 33-35 (2013). Xiangshao Granules are a TCM preparation for the treatment of women’s breast distending pains. For quality control, TLC on silica gel (1) for Cyperus rotundus, with petroleum ether (60-90 °C) – ethyl acetate 15:1, detection at UV 254 nm; (2) for Atractylodes macrocephala Koidz. and atractylone with petroleum ether (60-90 °C) – ethyl acetate 50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4, followed by heating until zones are visible; (3) for Angelica dahurica (Fisch. ex Hoffm.) Benth. et Hook. f. ex Franch. et Sav. and imperatorin with petroleum ether (60-90 °C) – diethyl ether 1:1, detection at UV 366 nm; (4) for Epimedium brevicornum Maxim and icariin with ethyl acetate – butanone – formic acid – water 10:1:1:1, detection at UV 366 nm before and after spraying with 3 % aluminium trichloride in ethanol.

Chinese J. of Northern Pharmacy 11(5), 14-16 (2014). Zhuanggu Tongluo Wan pills are a TCM preparation for the treatment of degenerative joint disease and osteoporosis. For quality control, TLC on silica gel (1) for Epimedium brevicornum Maxim. and the standard icariin, with ethyl acetate – acetone – methanol – water 20:2:3:2, detection at UV 254 nm; (2) for Paeonia veitchii and the standard paeoniflorin, with chloroform - ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible; (3) for aconitine, with chloroform - ethyl acetate 1:1 after exposure to ammonia vapor, detection by spraying with a solution of potassium iodobismuthate – hydrochloric acid – water 25:1:500 and evaluation in daylight.

J. Liq. Chromatogr. Relat. Technol. 38, 1097-1103 (2015). HPTLC of 15 polyoxygenated steroids on unmodified silica gel with non-aqueous binary solvent systems containing acetone – n-hexane or acetonitrile – dichloromethane. Lipophilicity parameters obtained by normal phase chromatography were validated in relation to corresponding reverse phase chromatographic data.

J. Planar Chromatogr. 28, 294-299 (2015). HPTLC of cucurbitacin B in Lagenaria siceraria on silica gel with chloroform - methanol 19:1. Quantitative determination by absorbance measurement at 242 nm. The hRF value for cucurbitacin was 67. Linearity was in the range of 200-1200 ng/zone. LOD and LOQ were 40 and 123 ng/zone, respectively. The intermediate precision was below 6 % (n=6). Recovery ranged between 95 and 96 %.