J. of Beihua Univ. (Natural Sci.), 11 (5), 420-423 (2010). TLC on silica gel with 1) petroleum ether (60-90 ºC) - ethyl acetate 4:1 and 2) chloroform - propanone - n-hexane - acetic acid 80:40:2:5. Detection under UV 365 nm. Derivatization by spraying with 10 % vanillin in sulfuric acid and heating at 105 ºC until the zones were visualized. Identification by comparison with the standards of the components in the individual composition drug.

62nd Indian Pharmaceutical Congress Abstract No. F-240 (2010). TLC of escitalopram oxalate on silica gel with n-butanol – acetic acid – water 3:1:1. Quantitative determination by absorbance measurement at 240 nm. The method was linear in the range of 100-600 ng/band.

Acta Chromatographica 21(4), 631-639 (2009). HPTLC on silica gel with toluene – ethyl acetate – formic acid 45:55:1. The hRf value was 31. Quantification by densitometry at 284 nm. The limit of quantification was 35 ng/band, recovery was 97.6-101.6 %, and precision 2.19 %RSD. The method was applicable for routine analysis and accelerated stability testing of terbinafine in pharmaceutical drug-delivery systems. It can be used as a stability-indicating method because it separated the drug from its degradation products.

F-255 (2010). TLC of irbesartan on silica gel with ethyl acetate – toluene – glacial acetic acid 35:15:1. Quantitative determination by absorbance measurement at 240 nm. The method was linear in the range of 200-800 ng/band. The sample was subjected to different stress conditions (acid, alkali, oxidation, thermal & photolytic). The compound was well separated from the different degradation products and could be estimated without any interference from the degradation product. The proposed stability indicating assay method was found suitable for routine quality control.

CBS 106, 2-4 (2011). TLC of milk samples on silica gel with dichloromethane - methanol - isopropanol - 25 % aqueous ammonia 3:3:5:2 in the horizontal DS chamber. Bioautography detection with Bacillus subtilis using the Chrom Biodip Antibiotic Kit and by dipping in a broth of Escherichia coli. Detection by spraying with an aqueous tetrazolium salt (MTT) solution of 0.2% and evaluation under daylight. With the E. coli assay the limit of detection for ciprofloxacin was 25 µg/kg, which is lower than with the Chrom Biodip test, while for enrofloxacin it was slightly higher (75 µg/kg).

J. Chinese Modern Med. & Pharm. 17 (34), 52-53 (2010). TLC on silica gel with chloroform – ethyl acetate – methanol – formic acid 2000:25:50:1. Detection under UV 366 nm. Identification of rhaponitin in both medicines by comparison with the standard.

J. of Guangzhou Chem. Engin. 39(1), 144-145 (2011). A course on the technology of TLC/bioautography applied for the analysis of TCM was set-up to enhance students’ understanding of theoretical knowledge and to train and improve the interest and skill of students in chemical experiments. Demonstration of the TLC analysis of rutin and quercetin in Flos Sophorae on silica gel with ethyl acetate – formic acid – water 8:1:1. Detection under UV 254 nm and 366 nm, and by immersing into a solution of 1,1-diphenyl-2-picrylhydrazyl in ethanol (DPPH radical reagent). The result of this practice was satisfactory, and the course proved to be a good example to utilize the modern technology in the experimental teaching.

J. Sep. Sci. 34, 2397-2404 (2011). TLC of polyaromatic hydrocarbons (PAH) to examine the influence of magnetic field on the retention of compounds on silica gel, with monocomponent mobile phases containing n-hexane, n-heptane, n-octane, benzene, and toluene and as binary phases, mixtures of n-hexane - benzene 9:1, 7:3, and 1:1. The magnetic field influences the retention and separation efficiency of investigated chromatographic systems.