J. Liq. Chromatogr. Relat. Technol. 35, 28-39 (2012). HPTLC of metformin hydrochloride and glipizide in tablet formulation on silica gel with water - methanol 3:1 + 1 drop ammonia. Quantitative determination by absorbance measurement at 236 nm. The hRf value of compounds (1) and (2) were 22 and 85 and selectivity regarding matrix was given. Linearity was in the range of 5000-25000 ng/band for (1) and 50-250 ng/band for (2). Limits of detection and quantification were 991 and 3003 ng/band for (1) and 10 and 29 ng/band for (2), respectively. Recovery (by standard addition) was between 98.1 and 101.5 % for both (1) and (2).

J. of China Pharm. 21 (10), 37-39 (2012). Changyanning oral liquid is a herbal TCM preparation with Euphorbia humifusa Willd as the key component drug. For quality control identification of Euphorbia humifusa Willd by TLC and quantification of its active component gallic acid by HPLC. TLC on silica gel with toluene – ethyl acetate – formic acid 5:2:1, detection by spraying with 1 % aluminium chloride in ethanol, evaluation under UV 366 nm.

Chinese J. of Guide for Trad. Chinese Med. & Pharm. 18 (5), 80-81 (2012). Weijianning capsules, a herbal TCM preparation for tonifying spleen and invigorating the stomach, is prescribed clinically to treat superficial, erosive and atrophic gastritis. Presentation of a TLC method for the quality control of the preparations optimized by investigating the sample extraction procedure and the mobile phases employed. TLC of the extracts on silica gel 1) for Radix Scrophulariae with ethyl acetate – ethanol – water 20:5:3, detection by spraying with a solution of 3 g vanillin – 100 mL ethanol – 0.5 mL sulfuric acid and heating at 105 °C until the zones were clearly visualized, evaluation in daylight; 2) for Fructus Amomi with n-hexane - ethyl acetate 3:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones were clearly visualized, evaluation in daylight. Identification by fingerprint comparison in parallel with the individual standard components.

J. Planar Chromatogr. 26, 153-159 (2013). TLC lipophilicities of 21 newly synthesized 1,2,4-triazoles on RP-8 and RP-18 with buffered solutions of acetone, 1,4-dioxane, tetrahydrofuran and acetonitrile as well as on cyano phase with solutions of 0.03, 0.04, 0.06, 0.08 and 0.1 M sodium dodecyl sulfate modified with the constant (20 %) addition of organic modifier (acetone, 1,4-dioxane, tetrahydrofuran and acetonitrile). Partitioning lipophilicity indices showed mobile phase composed of sodium dodecyl sulfate and tetrahydrofuran as the system providing the best linear correlations between chromatographic and partitioning lipophilicities of tested 1,2,4-triazoles.

J. Liq. Chromatogr. Relat. Technol. 36, 2497-2511 (2013). HPTLC evidence of rapid peptidization of L-proline and DL-proline on silica gel with 2-butanol - pyridine - ammonia (25 %) - water 39:34:10:26 and on silica gel plates impregnated with Mn(II) and Cu(II) acetate with dioxane - water 13:7+1 drop 2 % acetic acid. HPTLC enantioseparation of DL-proline was performed with 2-butanol - pyridine - glacial acetic acid - water 15:10:3:12. Detection by dipping into 0.5 % ninhydrin solution in 2-propanol, followed by heating for 2 min at 110 °C. Quantitative determination by absorbance measurement at 340 nm.

Beijing J. of Trad. Chinese Med. 31 (7), 542-545 (2012). Ekou powder is a herbal TCM preparation for treating mycotic and follicular stomatitis and mouth erosion. For quality control TLC on silica gel 1) for Coptis chinensis, with cyclohexane – ethyl acetate – isopropanol – methanol – water – triethylamine 6:7:2:3:1:2, detection under UV 366 nm; 2) for borneol, with n-hexane – ethyl acetate 17:3, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:200 and heating at 105 °C, viewing in daylight; 3) for Indigo naturalis, with toluene – chloroform – acetone 5:4:1, detection in daylight.

Chinese J. of Inform. on TCM 19 (9), 59-60 (2012). Some Aconitum medicinal herbs, such as Aconite root, Radix Aconiti Kusnezoffii, Radix Aconiti Lateralis Preparata etc. are used as the component drugs in TCM anti rheumatic preparations like Hulisan capsules, Yaoxitong capsules and Panlongqi tablets. The main active component in Aconitum is aconitine which has significant anti-inflammatory and analgesic activity, but is a potent toxic ingredient as well. For quality control of the formulations and ensuring their medication safety, a method for the limit test of aconitine in Hulisan capsules, Yaoxitong capsules and Panlongqi tablets has been presented. TLC on silica gel with cyclohexane – ethyl acetate – diethylamine 10:7:1, detection first by spraying with 5 % potassium iodobismuthate in water – hydrochloric acid 200:1 and then by exposing to iodine vapors, viewing in daylight. Semiquantification of aconitine by comparison of zones with the standard applied in concentrations meeting the safety limit.

Jiangxi J. of Trad. Chinese Med. 43 (350), 59-61 (2012). Fufang Sanqidan capsules are a herbal TCM preparation for treating frequent urination, cardiovascular and cerebrovascular diseases, nephropathy etc. For quality control TLC on silica gel for 1) Radix Notoginseng and Radix Astragali, with the upper phase of n-butanol – ethyl acetate – 10 % ammonia 4:1:5, 2) for Salvia miltiorrhiza, with toluene – ethyl acetate 19:1, detection by viewing in daylight; and 3) for Mulberry Fruit, with petroleum ether (30-60 °C) – chloroform – ethyl acetate 3:8:5. All were detectied by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C for 5 min, viewing under UV 366 nm. Quantification of astragaloside A by HPLC.