International Journal of Medical Toxicology & Legal Medicine 15 (1&2), 61-63 (2012). HPTLC for five benzodiazepines (alprazolam, clonazepam, diazepam, lorazepam and nitrazepam) on silica gel G with chloroform - acetic acid 9:1 in a presaturated TLC chamber. Detection by evaporisation with chlorine gas for 5 min, and after complete removal of chlorine, by spraying with o-tolidine reagent. Stabilisation of blue colored zones for a day by spraying the plate with 1 % phosphomolybdic acid. LOD for alprazolam, clonazepam, diazepan, lorazepam and nitrazepam was found to be 0.1, 0.5, 0.5, 1 and 0.5 µg/zone, respectively.

J. Planar Chromatogr. 26, 78-85 (2013). HPTLC of lamivudine (1), tenofovir disoproxil fumarate (2) and efavirenz (3) on silica gel with chloroform - methanol - toluene 30:4:1. Quantitative determination by absorbance measurement at 260 nm. The hRf values of (1), (2) and (3) were 20, 61 and 73, respectively. Linearity was in the range of 400-800 ng/zone for (1) and (2) and 800-1600 ng/zone for (3). Intermediate precision was below 2 %. LOD and LOQ were 180 and 300 ng/zone for (1), 150 and 210 ng/zone for (2) and 300 and 400 ng/zone for (3), respectively.

Chinese J. of Lishizhen Trad. Med. & Pharm. 22 (4), 829-830 (2011). Gongbaokang suppository is a traditional Chinese recipe for curing cervicitis, preventing pathological changes which may cause cervical carcinoma. Description of a method for the quality control. TLC of the extracts of the medicine 1) for Sophora flavescens, on silica gel with benzene – acetone – ethyl acetate – ammonia 10:15:20:1, detection by spraying with 5 % potassium iodobismuthate solution and viewing under daylight; 2) for Fructus Cnidii, on silica gel with toluene – ethyl acetate – n-hexane 3:3:2, detection under UV 366 nm; 3) for borneol, on silica gel with benzene – acetone 9:1, detection by spraying with 1 % vanillin in ethanol – sulfuric acid 4:1 and heating at 105 °C, viewing under UV 366 nm.

Chinese J. of Guide for Trad. Chinese Med. & Pharm. 18 (7), 76-78 (2012). Guanjieyan Babuji preparation is a herbal TCM for treating arthritis and joint diseases. For quality control, TLC on silica gel 1) for Amur Cork-tree Bark and the standard berberine hydrochloride, with toluene – ethyl acetate – methanol – isopropanol – water 60:30:20:15:3, detection by exposing to ammonia vapors and viewing under UV 366 nm; 2) for Fructus Psoraleae and the standards psoralen and angelicin, with n-hexane – ethyl acetate 4:1, detection by spraying with 10 % KOH in methanol and viewing under UV 366 nm 3) Radix et Rhizoma Rhei and the standard emodin, with petroleum ether (30-60 °C) – ethyl formate – formic acid 15:5:1, detection under UV 366 nm; 4) for Radix Astragali seu Hedysari, with chloroform – methanol 10:1, detection by exposing to ammonia vapors and viewing under UV 366 nm.

J. Planar Chromatogr. 26, 517-520 (2013). TLC of amino acids on silica gel with 1-propanol - water 7:3. Detection by spraying with (1) 1 % 2-hydroxybenzenecarbaldehyde solution in propanone; or (2) 1 % 4-bromobenzenecarbaldehyde solution in propanone (2), followed by heating at 110 ºC for 10 min. After cooling, all plates (treated with (1) or (2)) were sprayed with ninhydrin (indane-1,2,3-trione monohydrate) reagent and air dried. Amino acid zones in various shades from yellow to brownish-red were detected (detailed table provided in paper). Plates were heated again at 110°C for 10 min and color changes were recorded. The LOD were determined visually by comparison of standard solutions (1 mg/L) and further dilution until no zone was seen. LOD for (1) and (2) were in the range of 0.1-1.0 µg/zone and 0.01-1.0 µg/zone, respectively.

J. Planar Chromatogr. 26, 496-501 (2013). HPTLC of amitriptyline in gastric lavage on silica gel with methanol – ammonia (25%) 197:3. Quantitative determination by absorbance measurement at 209 nm. The hRf value of amitriptyline was 49. Linearity was between 10 and 250 ng/zone. LOD and LOQ were 5 and 17 ng/zone. Recovery (by standard addition) was in the range of 83-92 %. Intermediate intra- and inter-day precision was below 2 %.

Chinese J. of Northern Pharmacy 10 (10), 9-10 (2013). Keke Diwan drop pills are a herbal TCM preparation for the treatment of cough, dyspnea and shortness of breath. For quality control, TLC on silica gel 1) for Ephedra sinica Stapf and the standard ephedrine hydrochloride, with chloroform – methanol – ammonia 100:20:1, detection by spraying with 0.3 % ninhydrin in n-butanol – glacial acetic acid 19:1 and heating at 105 °C, viewing in daylight; 2) for Pericarpium papaveris, with toluene – acetone – ethanol – concentrated ammonia 20:20:3:1, detection under UV 366 nm; 3) for Glycyrrhiza uralensis Fisch., with benzene – chloroform – methanol – glacial acetic acid 40:90:15:1 with preconditioning for 10 min, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight.

J. Planar Chromatogr. 26, 435-439 (2013). HPTLC of ciclesonide in bulk and dosage form on silica gel with acetonitrile - 1,4-dioxane - n-hexane 1:1:8 +1 drop ethyl acetate. Quantitative determination by absorbance measurement at 242 nm. The hRf of ciclesonide was 38. Linearity was between 1 and 6 mg/zone. LOD and LOQ were 100 and 303 ng/zone. Recovery (by standard addition) was found to be 98.9-100.3 %. Intra- and inter-day precision values were below 0.7 and 1.4 %, respectively.