Chinese J. of Lishizhen Trad. Med. & Pharm. 22 (4), 860-862 (2011). Fallopia multiflora (Thunb.) Harald, a medicinal herbal drug widely applied in traditional Chinese medicines, is available from different provinces of China. Presentation of fingerprint methods of Fallopia multiflora from different regions by micellar thin-layer chromatography (MTLC) and IR techniques. MTLC of methanol extracts of Fallopia multiflora and emodin standard solution on polyamide layer with 0.4 % cetylpyridinium chloride in acetone – methanol – 1.0 mol/L NaOH 12:4:5 after chamber saturation for 15 min. Detection of the purple zones in daylight. The ratio of the hRf values of the component zones and those of emodin was calculated. In addition, FTIR of the methanolic extracts and emodin standard solution mixed in KBr tablets. The results of the real life samples available from five different regions are given and proved that the method is suitable for the quality control of the drugs.

J. of Inner Mongolia Univ. for Nationalities (Natural Sci. Edit.) 27 (1), 80-83 (2012). Yuzan Qingyan Shiwuwei pills are a traditional Mongolian herbal medicine for treating colds, sore throat, asthma, cough, etc. For quality control, TLC on silica gel 1) for Fructus Gardeniae and the standard gardenoside, with ethyl acetate – acetone – formic acid – water 5:5:1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 2) for Radix Aucklandiae and the standards costunolide and dehydrocostus lactone, with cyclohexane – formic acetate – formic acid 15:5:1, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:200 and heating mildly, viewing in daylight; 3) for Radix Glycyrrhizae and the standard glycyrrhetinic acid, with benzene – petroleum ether (30-60 °C) – ethyl acetate – glacial acetic acid 50:25:20:3, detection under UV 366 nm.

J. of Qilu Med. & Pharm. 31 (6), 339-340 (2012). Yinxie Granules are a herbal TCM for treating difficult skin diseases, like psoriasis, etc. For quality control, identification of Smilax china L. by TLC on silica gel with chloroform – ethyl acetate – methanol – water 3:8:3:2, detection under UV 366 nm. Quantification of astilbin by HPLC.

Chinese J. of Today’s Pharm. 22 (11), 659-663 (2012). Rulekang gel ointment is a herbal TCM for treating diseases of the mammary glands. For quality control, TLC on silica gel 1) for Nidus vespae, with n-hexane – chloroform – ethyl acetate 16:3:4, detection under UV 366 nm; 2) for Olibanum and Myrrh, with toluene – ethyl acetate 19:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C, viewing in daylight; 3) for Syzygium aromaticum, with petroleum ether ( 60-90 °C) – ethyl acetate 8:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C, viewing in daylight; 4) Rhizoma Curcumae Longae, with chloroform - methanol – formic acid 96:4:1, detection by viewing in daylight; 5) for Herba Asari, with n-hexane – ethyl acetate 4 1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C, viewing under UV 366 nm; 6) for Fructus Piperis Longi, with n-hexane – ethyl acetate – formic acid 600:400:1, detection under UV 366 nm. Quantification of curcumin by HPLC.

Chinese J. of Lishizhen Trad. Med. & Pharm. 23 (5), 1180-1181 (2012). Elaeagnus gonyanthes Benth. is a medicinal herb which grows in southern China. The crude drug is used in folk medicine for treating asthma, chronic bronchitis, arthritis, low back pain, traumatic swelling, etc. For identification, TLC of the extracts of different plant parts (leaf, stem) and the standards oleanic acid and ursolic acid on silica gel with cyclohexane – chloroform – ethyl acetate – glacial acetic acid 200:50:80:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight. Quantification of oleanic acid and ursolic acid by HPLC.

J. of China Pharm. 22 (1), 20-22 (2014). Ganmaoting Peifang granule is a herbal TCM for the treatment of cold, influenza, cough, sore throat, etc. For quality control, TLC on silica gel 1) for Fructus Arctii, with chloroform – methanol – water 40:8:1, detection by spraying with 10 % sulfuric acid in ethanol and heating mildly, viewing in daylight; 2) for Radix Isatidis, with n-butanol – glacial acetic acid – water 19:5:5, detection by spraying with 0.1 % ninhydrin in propanone and heating at 105 °C, viewing in daylight. Quantification of arctiin by HPLC.

J. Planar Chromatogr. 26, 336-342 (2013). HPTLC of diosmin (1), hesperidin (2), and ascorbic acid (3) on silica gel with ethyl acetate - methanol - water 15:3:2 for (1) and (2) and ethyl acetate - methanol - water - acetic acid 15:10:2:1 for (3). Quantification by absorbance measurement at 340 nm for (1), 286 nm for (2) and 265 nm for (3). The hRf values for (1), (2) and (3) were 34, 40 and 56, respectively. Linearity was in the range of 100-800 ng/zone for (1) and (2) and 50-400 ng/zone for (3). LOD and LOQ were 6 and 17 ng/zone for (1), 4 and 13 ng/zone for (2) and 4 and 13 ng/zone for (3), respectively. Recoveries were in the range of 98.1-99.3 % for (1), 98.4-99.5 % for (2) and 98.0-99.1 % for (3). Intermediate/interday/intra-day precision was below 2 % (n=6).

J. Chil. Chem. Soc. 58, 1663-1666 (2013). HPTLC of rosiglitazone (1) and glimepiride (2) in tablet dosage form on silica gel with methanol - toluene - ethyl acetate 1:8:1. Quantitative determination by absorbance measurement at 228 nm. The hRf values for (1) and (2) were 39 and 20, respectively. Linearity was in the range of 100-1500 ng/zone for (1) and 100-1500 ng/zone for (2). LOD and LOQ were 30 and 35 ng/zone for (1) and 85 and 90 ng/zone for (2), respectively. Recovery was 96 % for (1) and (2). Intermediate intra- and inter-day precision was below 2 % (n=6).