Abstract No. F-247, 62st IPC (2009). HPTLC of nebivolol HCl and S-amlodipine besylate on silica gel (prewashed with methanol) with chloroform - toluene - methanol - acetic acid 50:20:1. The hRf value was 33 and 48 for S-amlodipine and nebivolol, respectively. Quantitative determination by absorbance measurement at 271 nm. The method was linear in the range of 500-2500 ng/band for nebivolol and 250-1280 ng/band for S-amlodipine, respectively.

Chromatographia 70 (5-6), 1001-1006 (2009). Chiral TLC of the enantiomers of atenolol, propranolol, and salbutamol by complexation with Cu(II) cation and five L-amino acids using different techniques: 1) using the Cu(II)-L-amino acid complex as chiral mobile phase additive with untreated silica gel plates, 2) by mixing the Cu-complex with silica gel before preparing the TLC plates, 3) by development with solutions of the Cu-complex on untreated silica gel plates, and 4) by using a solution of Cu(II) acetate as mobile phase additive for plates prepared by mixing the L-amino acid with silica gel. Detection of zones by exposure to iodine vapor.

Abstract No. C-338, 61 IPC (2009). HPTLC of gallic acid and piperine in combined formulation on silica gel with toluene - ethyl acetate - formic acid 16:8:1. Quantitative absorbance measurement at 320 nm. The method was linear in the range of 200-800 ng/band for gallic acid and 50-350 ng/band for piperine.

Phytochem. Anal. 21, 219-223 (2010). HPTLC of safranal in saffron extract and in a safranal-loaded nanoparticle formulation on silica gel with n-hexane - ethyl acetate 9:1. Quantitative determination by absorbance measurement at 310 nm. The hRf of safranal was 51. Linearity was between 0.5 and 5.0 µg/zone. The intra-day and inter-day precisions were 1.08-2.17 and 1.86-3.47 %, respectively. LOD was 50 ng/zone while LOQ was 150 ng/zone. The average recovery was 99.9 %. The proposed method provides significant advantages in terms of greater specificity and rapid analysis.

Analytical Methods 2, 521-531 (2010). An HPTLC method for determination for paliperidone in formulation as well as for in vitro release studies has been developed. HPTLC on silica gel with methanol - ethyl acetate 4:1. The hRf value was 54. Quantification was performed by densitometric evaluation at 254 nm. The method was linear in the range of 100-600 ng/band. The method was suitable for estimation of the drug in mucoadhesive microemulsion formulations, as well as for solubility and diffusion studies.

International Journal of ChemTech Research 2(2), 851-855 (2010). Defatted powdered roots of Helicteres isora (Sterculiaceae) were extracted with ethyl acetate. TLC on silica gel with toluene - acetone - formic acid 25:5:2. The hRf value was 43. Densitometric evaluation at 540 nm. The method was linear in the range of 100-500 ng/band. Recovery was 98.4-99.9 %.

Biochem. Biophys. Res. Commun. 399, 716-720 (2010). HPTLC of globotriaosylceramide (Gb3) in mouse tissues on silica gel with chloroform - methanol - water 65:25:4. Detection by HPTLC-immunostaining with an anti-Gb3 monoclonal antibody. Quantification by densitometry with a luminescent image analyzer. The limit of detection of Gb3 was 50 ng/zone.

J. AOAC Int. 93, 1180-1191 (2010). TLC of tropisetron and its acid-induced degradation products on silica gel with methanol - glacial acetic acid 22:3 with chamber saturation for 45 min. Detection under UV light at 254 nm. Quantitative determination by scanning at 285 nm. The hRf values were 44, 84, and 92 for tropisetron and degradates I and II, respectively. Linearity was between 1 and 10 µg/zone. Mean accuracy was 100.2 %. The precision was 0.64 %.The limit of detection and quantification for tropisetron was 0.26 and 0.80 µg/zone, respectively. The intraday and interday precisions were evaluated by assaying freshly prepared samples of tropisetron in triplicate concentrations, i. e. 3, 5, and and 9 µg/zone, resulting in 99.8 %, 100.3 %, and 100.4 %, respectively, for interday precision and 99.7 %, 100.2 %, and 99.7 %, respectively, for intraday precision (with a precision of 0.9 %, 0.6 %, 0.8 % and 0.6 %, 0.4 %, 0.6 %, respectively).