Chinese J. of Northwest Pharm. 28 (3), 231-235 (2013). Berberis heteropoda Schrank, a Xinjiang special medicinal herb, is a perennial shrub, produced in northwest and southwest China, and is widely used as a herbal TCM drug. It is used for the treatment of dysentery, diarrhea, swollen eyes, eczema, etc. For quality control, identification of three alkaloids by TLC of the sample extracts and the standards berberine hydrochloride, palmatine chloride and jatrorrhizine hydrochloride on silica gel with toluene – ethyl acetate – methanol – isopropanol – ammonia 12:6:4:3:1 (determined by investigating three mobile phase systems), detection under UV 366 nm. In addition to optimization of the stationary phase, the developing system and the detection technique, the method was validated by investigating the effects of the sample application volume (2-3 μL), the developing temperature (room temperature) and the relative humidity (42-65 %).

by high-performance thin-layer chromatography) (Chinese). J. Chinese Trad. Patent Med. 35 (12), 2752-2754 (2013). Chimonanthus nitens Oliv. is a plant of the Calycanthaceae family growing in Jiangxi and Anhui areas of China. Pharmacological studies showed that the chemical constituents of the extracts of its dry leaves are volatile oils, alkaloids, flavonoids, coumarins, etc. The dry leaves are used clinically as the main ingredient drug in TCM prescriptions for the treatment of infantile cough, respiratory tract infection, hand foot and mouth disease and chronic pelvic inflammatory disease, etc. For quality control, HPTLC of the sample extracts and the standards cineole, linalool and beta-caryophyllene on silica gel firstly with chloroform – methanol 9:1 to 5 cm and then with petroleum ether (30-60 °C) – ethyl acetate 10:1 to 10 cm, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:4 and heating at 110 °C. Quantitative determination by densitometric evaluation at 606 nm for cineole and linalool and 545 nm for beta-caryophyllene using external standard calibration method. The content of the total unknown components is also given taking linalool as the calibration standard. Validation of the method by investigation of its linearity range (for cineole 0.7-11.4 µg/zone, r=0.990, n=7; for beta-caryophyllene 0.7-10.9 µg/zone, r=0.992, n=7; for linalool 1.8-18.0 µg/zone, r=0.990, n=7), precision (%RSD (n=6) 3.9 %, 1.5 % and 0.6 % for cineole, beta-caryophyllene and linalool, respectively), stability (within 30 min, %RSD (n=6) 2.0 %, 3.0 % and 3.3 % for cineole, beta-caryophyllene and linalool, respectively) and reproducibility (%RSD (n=6) 2.7 %, 4.4 % and 3.2 % for cineole, beta-caryophyllene and linalool, respectively). The recovery for cineole was 82.3-93.4 % (%RSD (n=3) 2.6-4.1 %), for beta-caryophyllene 86.1-96.7 % (%RSD (n=3) 2.2-3.6 %) and for linalool 95.8-109.8 % (%RSD (n=3) 1.9-2.9 %). Some unknown separated components need to be identified in a further study.

(L.) Merrill. J. Planar Chromatogr. 28, 48-53 (2015). HPTLC of isoflavones daidzein (1) and genistein (2) in Glycine max. on silica gel with chloroform - methanol 20:1. Quantitative determination by absorbance measurement at 254 nm. The hRF values of (1) and (2) were 26 and 42, respectively. Linearity was between 1 and 15 μg/mL. The intermediate intra-day and inter-day precisions were below 2 % (n=6). The LOD and LOQ were 130 and 430 ng/zone for (1) and 300 and 930 ng/zone for (2), respectively. Average recoveries for (1) and (2) were 97.5 % and 96.3 %, respectively.

J. Sep. Sci. 37, 2062-2068 (2014). HPTLC of (1) phospholipids phosphatidylcholine, (2) phosphatidylethanolamine, (3) phosphatidylinositol, (4) phosphatidylserine and (5) sphingomyelin in Phyrrhocoris apterus on silica gel with chloroform - methanol - acetic acid - water 60:35:2:1 over a path of 9 cm, and chloroform - methanol - acetic acid - water 25:15:4:2 over a path of 6 cm. Detection by exposure to iodine vapor, followed by detection at UV 205 nm. The hRF values of (1) to (5) were 91, 68, 82, 88 and 94, respectively. The spots were subsequently scrapped from the plates. Quantification by conversion into inorganic phosphate (as phosphomolybdate complex) followed by absorbance measuremet at 820 nm.

J. Planar Chromatogr. 28, 36-41 (2015). HPTLC of (1) gallic acid and (2) kaempferol during the processing of the Ayurvedic formulation Arista (herbal material fermented with flowers of Woodfordia fruticosa) on silica gel with toluene – ethyl acetate – formic acid – methanol 15:15:4:1. The hRF values of (1) and (2) were 50 and 58, respectively. _x000D_

Guide Chinese Med. 17, 85-87 (2014). Zhituo Shenfa Wan pills are a TCM preparation or the treatment of seborrheic alopecia and Alopecia areata. For quality control, TLC on silica gel 1) for Angelica sinensis and Ligusticum chuanxiong Hort, with n-hexane – ethyl acetate 19:1, detection at UV 366 nm; 2) for Atractylodes lancea (Thunb.) DC., with petroleum ether (60-90 °C) – ethyl acetate 19:1, detection by spraying with 5 % p-dimethylaminobenzaldehyde in sulfuric acid – ethanol 1:4 and evaluation under white light; 3) for Corydalis yanhusuo W. T. Wang ex Z. Y. Su et C. Y. Wu and the standard tetrahydropalmatine, with n-hexane – chloroform – methanol – triethylamine 10:6:1:1, detection by exposure to iodine vapors until the zones are clearly visualized, evaluation under white and at UV 366 nm; (4) for Pericarpium Citri reticulatae and the standard hesperidin, with chloroform – acetone – methanol 5:1:1, detection by spraying with 3 % aluminium trichloride in ethanol and heating mildly until the zones are visible under white and at UV 366 nm. Quantification of hesperidin by HPLC.

Chinese J. Inform. Trad. Chinese Med. 20 (5), 67-69 (2013). Yinao Huoxue Keli granule is a TCM compound for the treatment of hemiplegia, numbness of limbs, deviation of mouth and tongue. For quality control, HPTLC on silica gel (1) for Salvia miltiorrhiza Bunge with chloroform – acetone – formic acid 8:1:1, detection by spraying with 3 % ferric chloride in 2 N hydrochloric acid – water 1:100 and heating at 105 °C until the spots were visible, identification by fingerprint comparison with the standard protocatechuic aldehyde and the standard ingredient drug; (2) for Panax quinquefolius L., ginsenoside Re, Rb1 and pseudoginsenoside F11 with the lower phase of chloroform – ethyl acetate – methanol – water 15:40:22:10 placed at 5-10 °C for 12 h, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the spots were visible.

J. Planar Chromatogr. 28, 234-240 (2015). HPTLC of (1) omeprazole, (2) pantoprazole, (3) rabeprazole and (4) diclofenac from tablets on silica gel previously washed with methanol and activated at 105 °C for 20 min, with toluene - n-butanol - 25 % ammonia 30:70:2. Quantitative determination by absorbance measurement at 290 nm. The hRF values were 67 for (1), 38 for (2), 57 for (3) and 27 for (4). Linearity was between 50 and 500 ng/zone for (1), (2) and (3), and between 150 and 1500 ng/zone for (4). The interday and intraday precisions were below 2 %. The LOD and LOD respectively for (1) were 7 ng/zone and 24 ng/zone, for (2) 9 ng/zone and 30 ng/zone, for (3) 13 ng/zone and 43 ng/zone, and for (4) 25 ng/zone and 82 ng/zone. Recovery was 99.6 % for (1), 100.1 % for (2), 100.4 % for (3) and 100.1 % for (4).