J. Planar Chromatogr. 24, 312-315 (2011) HPTLC of methanolic extracts of L. tibetica on silica gel (prewashed with methanol) with hexane - ethyl acetate - formic acid 12:7:1 for three furofuranolignans (sylvatesmin, (+)-piperrtol, horsfieldin), beta-sitosterol, and oleanolic acid, and with chloroform - methanol - water - formic acid 70:25:4:2 for four furofuranolignans (phillyrin, tibeticoside A, lantibeside C, and lantibeside), verbascoside and isoverbascoside in a twin-trough chamber with saturation for 20 min. Detection by immersion in ethanolic sulfuric acid for 2 s followed by heating for 5 min at 100 °C. The hRf values were 17, 25, 29, 51, 60, 70, 66, 49, 46, 19, and 21 for sylvatesmin, (+)-piperrtol, horsfieldin, oleanolic acid, beta-sitosterol, phyllirin, tibeticoside A, lantibeside C, lantiboside, verbascoside and isoverbascoside, respectively.

Part III – a study on CN adsorbent layers. J. Planar Chromatogr. 25, 471-474 (2012). TLC of 35 simple compounds with known literature lipophilicity on cyano phase with increasing mixtures of methanol - water. Detection by absorbance measurement at 254 nm. Correlation between single RM values and lipophilicity values as well as dependences between the extrapolated log kW and experimental lipophilicity was described.

J. of Chromatogr. A 1248, 169-177 (2012). Purified oligomers of hyalobiuronic acid are indispensable tools to elucidate the physiological and pathophysiological role of hyaluronan degradation by various hyaluronidase isoenzymes. Establishment and validation of a novel sensitive, convenient, rapid, and cost-effective HPTLC method for the qualitative and quantitative analysis of small saturated hyaluronan oligosaccharides consisting of 2–4 hyalobiuronic acid moieties. HPTLC on amino phase with 1-butanol - formic acid - water 3:5:2 or 3:4:1. Detection 1) by spraying with orcinol in various concentrations of sulfuric acid; 2) by dipping into the reagent of orcinol in 10 % sulfuric acid and Morgan–Elson reagent; 3) by illuminating with white light and UV 366 nm after heating. The simple reagent-free in situ derivatization of 3) resulted in a detection limit of 7–19 pmol/band and LOQ of 37–71 pmol/band depending on the analyzed saturated oligosaccharide. Identification of the analytes by TLC-ESI-MS. The validated HPTLC method, as an alternative to sequential techniques such as HPLC and CE, can easily be automated and is applicable to the analysis of multiple samples in parallel.

J. of Chinese Med. 26 (160), 1088-1090 (2011). Dangshi tablets are a herbal TCM preparation for cleaning heat, diuresis, and dissolving stones, and are prescribed to cure urinary tract infections and acute nephritis. For quality control, TLC of the extracts of the medicine on silica gel 1) for Poria cocos, with petroleum ether (30-60 ºC) – acetone – ethyl acetate 85:15:1, detection at UV 366 nm; 2) for Licorice, with chloroform – methanol – water 40:10:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ° until the zones are visible, evaluation in daylight.

J. Strait Pharm. 24 (5), 36-38 (2012). Glucosamine sulfate is a kind of glycosaminoglycan, which, as a medicine, is able to stimulate cartilage cells, to supplement the cartilage matrix and to inhibit matrix metalloproteinase expression, thus to promote the repair of cartilage. For better quality control of the medicine a method is presented for the analysis of the related substances in glucosamine sulfate potassium chloride. TLC on silica gel with dichloromethane – methanol – ammonia 2:2:1, detection by exposure to iodine vapors for 30 min and evaluation in daylight. The LOD was 25 µg/mL. Investigation of the stability of the medicines by analysis of a sample submitted to typical stress conditions such as acid, alkali, water bath, hydrogen peroxide, high temperature and UV light. Degradation occurred only for the samples treated with alkali and in the water bath.

J. Liq. Chromatogr. Relat. Technol. 35, 1676-1685 (2012). HPTLC of ranitidine hydrochloride thermal decomposition products on silica gel with dimethyl sulfoxide (DMSO), acetonitrile, methanol, 25 % ammonia, 2-propanol, and 2-methoxyethanol in a concentration range of 0-100 %. Detection under UV light at 254 nm. Degradation products with the binary mobile phases are arranged in three groups, depending on the retention: acetonitrile/DMSO at 373K (product 3), acetonitrile/DMSO at 353 K (product 3), and 2-methoxyethanol/DMSO at 353 K and 373 K (product of 2 and 3).

Chromatogr. 26 (1), 61–68 (2012) Picroside-I and picroside-II are known bioactive metabolites in Picrorhiza species. Presentation of a simple, precise method for the simultaneous determination of picrosides (picroside-I and picroside-II) in two different Picrorhiza species, P. kurroa and P. scrophulariiflora. TLC of the extracts of the medicinal herbal drugs on silica gel with chloroform – methanol 22:3. Quantification of picrosides (picroside-I and picroside-II) by absorbance measurement at UV 254 nm. Comparative study revealed that the content of picroside-I and picroside-II is higher in P. scrophulariiflora than P. kurroa the content of picroside-I was found to be 1.3 and 1.6 % inP. kurroa and P. scrophulariiflora, and of picroside-II 0.5 and 0.6 %, respectively. Study of the antioxidant potential of the two Picrorhiza species using DPPH* radical reagent. The scavenging activities of P. kurroa and P. scrophulariiflora were 37.7 % and 34.3 %, respectively, at a concentration of 0.1 mg/mL.

J. of China Pharm. 21 (10), 26-27 (2012). Yifeitongluo granules are a herbal TCM preparation for the combined treatment of deficiency of both qi and yin. TLC of the extracts of the preparations on silica gel 1) for Rhizoma Polygonati, with chloroform – methanol – acetic acid 5:4:1, detection by spraying with 2.5 % phosphomolybdic acid in ethanol and heating at 105 °C until the zones are visible; 2) for Viola yedonensis Makino, with toluene – ethyl acetate – formic acid 5:3:1, detection under UV 366 nm; 3) for Stemona sessilifolia (Miq.) Miq, with toluene – propanone – methanol 16:6:1 after preconditioning with ammonia vapors, detection by spraying with 5 % potassium iodobismuthate and viewing in daylight.