J. of China Pharm. 27 (7), 722-724 (2013). Xihuang Wan pill is a herbal TCM prescribed for treatment and adjuvant therapy of various cancers. Huoxue Zhitong San powder is a herbal TCM prescribed for traumatic injury and ecchymoma. In the drug market fraud rosin was found to be added to the key ingredient olibanum. For quality control, HPTLC of the sample extracts and the standards acetyl-11-keto-beta-boswellic acid (AKBA), 11-keto-beta-boswellic acid (KBA), beta-boswellic acid (beta-BA), acetyl-beta-boswellic acid (beta-ABA), and abietic acid (LOD=1.0 µg), on silica gel with toluene – ethyl acetate – heptane – formic acid 80:20:10:3 with chamber saturation for 20 min, detection 1) under UV 254 nm and UV 366 nm; 2) by spraying with a fresh solution of anisaldehyde – sulfuric acid – acetic acid 1:10:1000 and heating at 105 °C until the zones were visible in daylight. Verification of abietic acid from added rosin by HPLC. The method was used for the analysis of 63 batches of Xihuang Wan pills sampled from 13 pharmaceutical factories and 10 batches of Huoxue Zhitong San powder from 4 factories.

J. of China Pharm. 22 (3), 13-15 (2013). Gushangling Jiaonang capsule is a herbal TCM for post traumatic functional recovery, prescribed to treat muscle atrophy, bone hyperplasia, neck and lumbar diseases, nerve injuries etc. For quality control, HPTLC on silica gel 1) for Paeonia lactiflora Pall., with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 3 % vanillin in sulfuric acid – water 1:200 and heating mildly until the zones are visible in daylight; 2) for Angelica sinensis, with n-hexane – ethyl acetate – 9:1, detection under UV 366 nm; 3) Astragalus membranaceus (Fisch.) Bunge., with chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible in daylight.

J. Planar Chromatogr. 27, 460-465 (2014). HPTLC of (1) schisandrin, (2) gomisin B, (3) deoxyschisandrin and (4) gomisin N on silica gel with toluene – ethyl acetate – methanol 6:1:1. Quantitative determination by absorbance measurement at 225 nm. The hRF values of (1) to (4) were 20, 32, 61 and 72, respectively. Linearity was between 10 and 500 ng/zone for each substance. The intermediate intra-day and inter-day precision was below 3 % (n=5) for (1) to (4). The LOD and LOQ were 2.0 and 6.6 ng/zone for (1) and (2), and 2.5 and 8.2 for (3) and (4), respectively. Recovery for (1) to (4) were in the range of 97.2-102.3 %.

J. Liq. Chromatogr. Relat. Tech. 37, 2989-2999 (2014). HPTLC of neutral and polar lipid content of digestive gland-gonad complex of Biomphalaria glabrata on silica gel with petroleum ether - diethyl ether - glacial acetic acid 80:20:1 for neutral lipids, and chloroform - methanol - deionized water 65:25:4 for phospholipids. Detection of neutral lipids by spraying with 5 % ethanolic phosphoric acid, followed by heating at 115 ºC for 10 min. Phospholipids were detected by spraying with 10 % cupric sulfate in 8 % phosphoric acid, followed by heating at 140 ºC for 30 min. Quantitative determination by absorbance measurement at 610 nm for neutral lipids and 370 nm for polar lipids. The hRF values of neutral lipids were 19 for cholesterol, 38 for oleic acid and 57 for triolein, whereas for polar lipids were 46 for phosphatidylcholine and 66 for phosphatidylethanolamine.

J. Planar Chromatogr. 28, 83-89 (2015). HPTLC of irbesartan in tablets and plasma on silica gel with acetonitrile - chloroform - glacial acetic acid 30:19:1. Detection by exposure to concentrated hydrochloric acid vapor for 10 min. Quantitative determination by fluorescene measurement at 255/>400 nm. The hRF value of irbesartan was 40. Linearity was between 7 and 90 ng/zone. The intermediate intra-day and inter-day precisions were below 2.8 % (n=6). The LOD and LOQ were 2 and 6 ng/zone, respectively. Recoveries were in the range of 96.9-98.6 % in tablets and 95.8-97.4 % in plasma.

J. Planar Chromatogr. 27, 385-391 (2014). TLC fingerprint of wound-induced secondary metabolites in Passiflora foetida on silica gel with chloroform – ethyl acetate – glacial acetic acid – benzene 25:15:2:10. Qualitative identification at UV 366 nm. The method allowed the characterization of 10 secondary metabolites with hRF values between 10 and 94.

J. Beijing Univ. Agr. 29 (2), 33-35 (2014). Qishen Koufuye oral solution is a TCM preparation for the treatment of chronic gastritis, gastric and duodenal ulcer, etc. For quality control, TLC on silica gel (1) for Astragalus membranaceus (Fisch.) Bunge. and astragaloside A with ethyl acetate – butanone – formic acid – water 5:3:1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C for 5 min, evaluation under white light and at UV 366 nm; (2) for Atractylodes macrocephala Koidz. with cyclohexane - ethyl acetate 7:3, detection by spraying with 5 % p-dimethylaminobenzaldehyde in sulfuric acid – ethanol 1:4 and heating at 105 °C for 5 min, evaluation at UV 366 nm; (3) for Glycyrrhiza uralensis Fisch. with ethyl acetate – formic acid – glacial acetic acid – water 15:1:1:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C for 5 min, evaluation under white light and at UV 366 nm. Identification of Radix Codonopsis by HPLC.

J. Liq. Chromatogr. Relat. Technol. 38, 1113-1120 (2015). HPTLC of orbifloxacin (1), danofloxacin (2), ciprofloxacin (3), norfloxacin (4), enrofloxacin (5), marbofloxacin (6), difloxacin (7) and ofloxacin (8) on silica gel with 1,4-dioxane - ammonia - tetrahydrofuran 6:3:2. Quantitative determination by absorbance measurement at 320 nm. The hRF value was 19 for (4), 23 for (3), 30 for (2), 37 for (8), 40 for (6), 44 for (5), 56 for (1) and 59 for (7). Linearity was in the range of 159-513 ng/zone for (1), 99-238 ng/zone for (2), 97-244 ng/zone for (3), 69-163 ng/zone for (4), 63-170 ng/zone for (5), 103-250 ng/zone for (6), 84-251 ng/zone for (7) and 94-260 ng/zone for (8). LOD and LOQ were 53 and 159 ng/zone for (1), 32 and 98 ng/zone for (2), 32 and 97 ng/zone for (3), 23 and 69 ng/zone for (4), 21 and 63 ng/zone for (5), 34 and 103 ng/zone for (6), 28 and 84 ng/zone for (7) and 31 and 94 ng/zone for (8), respectively. Selection of appropriate viscosity of the mobile phase may be important in obtaining optimal chromatographic separation.