J. of Guangzhou Chem. Engin. 39(1), 144-145 (2011). A course on the technology of TLC/bioautography applied for the analysis of TCM was set-up to enhance students’ understanding of theoretical knowledge and to train and improve the interest and skill of students in chemical experiments. Demonstration of the TLC analysis of rutin and quercetin in Flos Sophorae on silica gel with ethyl acetate – formic acid – water 8:1:1. Detection under UV 254 nm and 366 nm, and by immersing into a solution of 1,1-diphenyl-2-picrylhydrazyl in ethanol (DPPH radical reagent). The result of this practice was satisfactory, and the course proved to be a good example to utilize the modern technology in the experimental teaching.

CBS 107, 9-10 (2011). Extraction of pesticides from fruit and vegetable samples by QuEChERs method. TLC of acetamiprid, azoxystrobin, chlorpyriofos, fenarimol, mepanipyrim, penconazole and pirimicarb on amino phase aluminum foil (prewashed with acetonitrile) with acetonitrile over a migration distance of 75 mm in the first direction. After drying development in the backwards direction over 45 mm with acetone. Evaluation under UV 254 nm, UV 366 nm, white light and under UV 366 nm after immersion in primuline solution. Extraction of the target zone by TLC-MS interface with acetonitrile - 10 mM ammonium formate 1:1. Average recoveries of the seven pesticides were 90-104 % with %RSD of 0.3-4.1 % (n = 5). This new high-throughput planar solid phase extraction method for multi-residue analysis of pesticides in food allows a rapid and efficient clean-up at low costs and low solvent consumption.

J. Planar Chromatogr. 23, 353-358 (2010). TLC of aclarubicin and doxycycline on silica gel, RP-18, cellulose, polyamide 11, and HPTLC on silica gel and RP-18 with a wide range (from 0 to 100 %) of mixtures of n-alcohols with DMSO, hexamethyldisiloxane, acetonitrile, and water in chambers saturated with mobile phase at room temperature. Detection by spraying with sulfuric acid - methanol 1:4, anisaldehyde - methanol - acetic acid - orthophosphoric acid - sulfuric acid 1:100:10:10:5 (for cellulose) and 5 % aluminium chloride in methanol (for polyamide). The effect of mobile and stationary phases on the chromatographic behavior of the compounds was studied.

Chinese J. Ethnomed. Ethnopharm. 1, 55-66 (2011). TLC of the extracts of Baibanting 1) for Gardenia jasminoides Ellis, on silica gel with ethyl acetate - acetone – methanol – water 5:5:1:1, detection under daylight and by spraying with 10 % sulfuric acid in ethanol and heating at 110 ºC; 2) for Cuscuta chinensis Lam, on polyamide phase with methanol - glacial acetic acid - water 4:1:5, detection by spraying with 3 % alumnium chloride in ethanol, evaluation under UV 366 nm; 3) for Malaytea scurfpea fruit, on silica gel with n-hexane - ethyl acetate 4:1, detection by spraying with 10 % KOH in methanol and evaluation under UV 366 nm. Quantification of gardenoside by HPLC. The procedures proved to be simple, accurate, reproducible, robust and suitable for the quality control of the medicine.

J. Liq. Chromatogr. Relat. Technol. 32, 2893-2905 (2009). HPTLC of albiflorin, paeoniflorin, (beta)-catechin, benzoyloxypaeoniflorin, benzoylpaeoniflorin, beta-sitosterol in the roots of Radix paeoniae Rubra (1) and Radix Paeoniae Alba (2) on silica gel with chloroform - ethyl acetate - methanol - formic acid 30:5:10:1 for high polarity components and toluene - ethyl acetate - methanol - formic acid 20:4:2:1 for lipophilic components. Detection by spraying with vanillin - sulphuric acid - ethanol 1:5:95, followed by heating at 105 ºC for 10 min. Qualitative determination by densitometry at 366 nm. The HPTLC fingerprints allowed differentiation between the roots of (1) and (2).

Food Chemistry 132, 671-674 (2012). TLC of saponins on silica gel with n-butanol - water - acetic acid 12:2:1. Detection by dipping into a suspension of sheep erythrocytes for 20 s, then plates were taken out and held vertically for 30 s. White spots against a pink background appeared. The plate was immersed in phosphate-buffered saline for 30 s to remove excess blood on the plate surface and again held vertically for 30 min. The method is simple, specific, convenient and time saving for analysis of saponins by TLC for purification, chemoprofiling of plants, and nutraceutical applications.

J. Planar Chromatogr. 24, 348-351 (2011). Study of the lipophilicity and specific hydrophobic surface area of fourteen 1,3-benzoxazol-2(3H)-ones substituted in the benzene ring (fluoro-, chloro-, bromo-, dibromo-, amino-, and nitro-derivatives). TLC on RP-18 with methanol - water, methanol - aminoacetic acid buffer pH 2.7, and methanol - aminoacetic acid buffer pH 11.6. The concentration of methanol in the mobile phase ranged from 30-90 % in all cases. Detection under UV 254 nm. The linear correlation between the volume fraction of methanol and values over a limited range were established with good correlation coefficients (r > 0.98). The obtained results were compared with calculated partition coefficients.

J. of Chromatogr. A 1251, 240-243 (2012). Report of the use of silver-thiolate chromatographic material as a stable material for TLC. The stationary phase operated under the same principles as silver-ion chromatography, separating compounds by degree of unsaturation; however, showed considerable advantages over Ag-TLC in terms of light stability and shelf lifetime. Demonstration of the light stability and its application for separations based on the degrees of unsaturation. TLC of using fatty acid methyl esters (FAMEs) with hexane – ethyl acetate 9:1 and of polycyclic aromatic hydrocarbons (PAHs) with 100 % ethyl acetate.