J. Chromatogr. A 1533, 199-207 (2018). Presentation of a simple and cost-effective approach for the determination of selenium species in algae and yeast biomass by TLC combined with diode laser thermal vaporization inductively coupled plasma mass spectrometry (DLTV ICP MS), after extraction of freeze-dried biomass in 4M methanesulphonic acid and vaporization of the selenium species from cellulose plates using a continuous-wave infrared diode laser with power up to 4W. Quantification of selenomethionine and selenocysteine with LOD of 3 μg/L in a Se-enriched microalgae Chlorella vulgaris and yeast certified reference material SELM-1. Comparison of the results delivered by TLC-DLTV ICP MS with those obtained by a routine coupling of HPLC to ICP MS showed that both were consistent, moreover, the TLC approach has advantage in capability of analyzing extract containing even undiluted crude hydrolysates.

J. Chromatogr. A, 1572, 145-151 (2018). Introduction of on-surface reactions as a new strategy for rapid structure elucidation. This was illustrated by a miniaturized on-surface synthesis-guided identification of two new degradation products (impurities) occurring in a pharmaceutical formulation of the anti-cancer drug ifosfamide, especially in the presence of urea. The respective reagents were applied in the nanomole scale accurately and automated on a HPTLC silica gel plate. After a fast reaction in the start zone, the plate was developed, followed by online elution to high-resolution MS, whereby the on-surface reaction highlighted the impurities. As proof of concept and for benchmarking, it was compared to a reaction mixture obtained from conventional preparative synthesis in a round-bottom flask as well as to different formulations. Image evaluation was performed by videodensitometry. Discussion of the advantages such as: 1) the combination of all relevant steps on one HPTLC plate and its resulting efficiency made surface synthesis on chromatographic phases an optimal tool for signal highlighting in MS, and thus for the assignment of impurities in drugs; 2) the miniaturization of the chemistry process scale down to the μg-level per synthesis (in total 30-60 μg chemicals/reaction), setting a new state-of-the-art standard; 3) the contribution to a greener chemistry by reducing the consumption of chemicals and enhancing the analytical efficiency, when adapted for the quality control of any other chemical product.

J. Chromatogr. A 1569, 212-221 (2018). Presentation of a method for simultaneous determination of mono-, di-, oligo- and polysaccharides in food by HPTLC on silica gel with acetonitrile – water 4:1 containing 3.6 mM natural products reagent A in a single development. Use of the method for both, effective food screening and quantification for up to 20 samples in parallel on the same plate with good reproducibility of the separation at control of the layer activity, and with the determination coefficients of the calibration curves between 0.9980 and 0.9998. Test of the method with 4 different prebiotic food and 60 naturally degraded inulin samples proved its advantages of minimal sample preparation along with the determination of the intact inulin and FOS allowed the evaluation of the natural inulin degradation profile, as shown for naturally degraded inulin samples, and the method is robust and suitable for routine analysis, especially in food control, as well as might be of interest in other fields, e.g. plant breeding, edible insects, functional feed and metabolic processes.

J. Chromatogr. 318, 450-454 (1985). TLC of anthocyan aglycones, monoglycosides, diglycosides and triglycosides on cellulose with conc. HCl - formic acid water 30.5:7.7:61.5; 30.8:27.8:41.4; 24.9:23.7:51.4 and 7 other solvents. Rf values of 27 compounds in 11 solvent systems are listed. The solvent composition 24.9:23.7:51.4 has the advantages of resolving, within a reasonable Rf range, aglycones, monoglycosides and diglycosides.

J. Agric. Food Chem. 32, 691-693 (1984). Determination of hemolytic activity of saponins on TLC plates, coated with blood-gelatin suspension, after separation on silica, using chloroform-methanol - water 25:17:3 and 65:35:10 (bottom layer). New detection method for hemolytic activity directly on the TLC plate.

J.A.O.A.C. 67, 563-565 (1984). TLC of chloramphenicol on silica with ethyl acetate. Detection: 2 minutes exposure in jar containing solid calcium hypochlorite, 45 sec. in jar containing 37 % formaldehyde, spray lightly with aq. solution containing 1 % starch + 1 % KI. Detection limit: > 60 ng.

Biochem. System. and Ecology 12, 183-188 (1984). TLC on silica with ethyl acetate - isopropanol - water 65:22:11; visualization by spraying with 85 % phosphoric acid - acetic acid - aniline - diphenylamine 20 ml + 100 ml + 5 ml + 5 g and heating at 100 °C for 2-3 h. Identification of sugars by co-chromatography with authentic standards.

Shoyakugaku Zasshi 37, 195-197 (1983). TLC of alkaloids in 21 coptis rhizomes on silica with benzene - ethyl acetate - propanol - methanol - ethylamine 8:2:2:1:1 for protoberberine alkaloids without phenolic-OH groups, and methanol - water NH3 8:1:1 for those with phenolic-OH groups. Detection by UV.