Food Chem. 210, 200-211 (2016). Review of the principles and applications of matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) in food science and related fields. The review described the combination of MALDI-MSI and HPTLC, including the visualization of phospholipids in chicken egg yolk on silica gel with acetonitrile – water 1:1.

Phytochem. Anal. 27, 222-228 (2016). HPTLC fingerprinting of luteolin, quercetin and their corresponding glycosidic derivatives luteolin-7-O-glucoside and rutin (quercetin-3-O-rutinoside) in Soldanella alpina on silica gel with formic acid – water – ethyl methyl ketone – ethyl acetate 1:1:3:5. A solution of 2,5-dihydroxybenzoic acid in 70 % methanol (100 mg/mL) was used as a matrix for HPTLC-MALDI-TOF MS analysis.

Rev. Colomb. Cienc. Quim. Farm. 45, 147-168 (2016). HPTLC of propolis based lipid nanoparticles containing tea tree (Melaleuca alternifolia) on silica gel with toluene – ethyl acetate 93:7. Qualitative determination under UV light at 365 nm. Detection by spraying with anisaldehyde-sulfuric acid reagent (0.5 mL anisaldehyde was mixed with 10 mL glacial acetic acid, followed by 85 mL methanol and 5 mL concentrated sulfuric acid).

J. Liq. Chromatogr. Relat. Technol. 39, 281-285 (2016). HPTLC bioautography of Potentilla species on silica gel with diethyl ether – methanol – formic acid 150:50:5:1. Direct bioautography by dipping into a bacterial suspension of Bacillus subtilis for 8 s, following incubation at 37 °C for 17 h. Visualization by spraying with 0.2 % MTT (thiazolyl blue tetrazolium bromide) aqueous solution, followed by incubation at 37 °C for 0.5 h.

J. of China Pharm. 23 (2), 48-51 (2014). Qingbu Tongluo Wan pill is a herbal TCM preparation for the treatment of acute and chronic brucellosis. For quality control, TLC on silica gel (1) for Fructus aurantii, with chloroform – formic acid – water 13:6:2, detection by spraying with 3 % aluminium chloride in ethanol and heating at 105 ºC, detection under UV 366 nm; (2) for Salvia miltiorrhiza Bge., with petroleum ether (60-90 ºC) – ethyl acetate 4:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC, evaluation in daylight; (3) for Corydalis yanhusuo W. T. Wang ex Z. Y. Su et C. Y. Wu, with toluene – acetone 9:2, detection by exposing to iodine vapors for 3 min, evaluation under UV 365 nm; (4) for Radix paeoniae rubra, with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating mildly until the zonesare visible in daylight. Quantification of salvianolic acid B by HPLC.

J. Agric. Food Chem. 64, 9203-9213 (2016). HPTLC of transglycosylated products of epigallocatechin gallate using dextransucrase on silica gel with acetonitrile – water 17:3. Detection by dipping into a mixture of 0.5 % N-(1-naphthyl)ethylenediamine and 5 % sulfuric acid in methanol, followed by heating at 125 °C for 5 min.

J. of Chromatogr. A 1441, 126-133 (2016). Presentation of a method for the analysis of ergot alkaloids including an ammonium acetate buffered extraction step, followed by a fast liquid-liquid partitioning pre-cleaning and then planar solid phase extraction (pSPE). HPTLC on amino phase with methanol for separation of the ergot alkaloids from the remaining matrix and for focusing them in a single zone. Quantification after dipping the plate in n-hexane – paraffin solution for fluorescence enhancement. The LOD and LOQ was 0.07 and 0.24 mg/kg rye, respectively, expressed as ergocristine, which was well below the currently applied quality criteria limit for rye. The recovery was almost 100 % at relevant spiking levels for different rye flour samples. The pSPE–FLD method was fast, efficient and reliable for screening the total ergot alkaloid content in rye and it was a rapid alternative to the HPLC determination with summing up the individual alkaloids. Furthermore, HPTLC-MS additionally enables the identification of the ergot alkaloid composition by a single mass spectrum, when utilized as a fingerprint, offering an easy differentiation of Secale cornutum from different origins.

J. Ethnopharmacol. 197, 10-24 (2017). Review of various aspects for the quality control of Ayurvedic herbs, including standardization, chemical-profiling and metabolite fingerprinting. A comprehensive table with important parameters for quality evaluation of Ayurvedic medicine was presented, including TLC and HPTLC profile with markers.