CBS 100, 2-5 (2008). HPTLC of photodegraded UV filters and sunscreen on silica gel LiChrospher prewashed with methanol. AMD 2 development of UV filter standards photodegradation products with diisopropylether - n-hexane in 6 steps over 50 mm without preconditioning, and of sunscreen samples photodegradation products with t-butylmethylether - n-hexane in 7 steps over 50 mm with preconditioning, followed by drying at 120 °C for 30 min. Detection at 254 and 366 nm, followed by biodetection via dipping the plate in a Vibrio fischeri solution for 1 s and evaluation with the Bioluminizer (exposure time 55 s). Densitometric evaluation by multi-wavelength scan at 200-400 nm.

J. Planar Chromatogr. 21, 135-141 (2008). Quantitative structure-retention relationships and quantitative structure-activity relationships have been used to study the chromatographic behavior and the antibacterial activity of different substituted benzimidazole derivatives. HPTLC of eleven benzimidazole derivatives on RP-18 with methanol - phosphate buffer (pH 7 resp. pH 8). Evaluation under UV 254 nm.

J. Liq. Chromatogr. Relat. Technol. 30, 2221-2229 (2007). HPTLC of acetic, fumaric, lactic, malic, pyruvic, and succinic acid on 1) cellulose with n-propanol - 2 M ammonium hydroxide 7:3 (triple development) in a twin-trough chamber with chamber saturation, detection with aniline-xylose reagent; 2) on silica gel with n-butyl formate - 90 % formic acid - water 7:2:1, detection with ethanolic bromocresol green reagent; 3) on cellulose with water-saturated isopropyl ether - formic acid 3:1 containing 2 - 3 mg/100 mL dichlorofluororescein, detection with pyridine vapor and evaluation under UV; 4) on silica gel with n-pentyl formate - chloroform - formic acid 14:3:3 or 2:7:1, detection with bromocresol green reagent; 5) on silica gel with diisopropyl ether - formic acid - water 16:3:1, detection with bromophenol blue reagent; 6) on silica gel with diisopropyl ether - formic acid - water 90:7:3, detection with aniline - glucose reagent, or bromocresol green, bromophenol blue, bromocresol purple, or potassium permanganate reagents. Best results were obtained with method 1 on cellulose.

J. Liq. Chromatogr. Relat. Technol. 31, 763-771 (2008). TLC of 23 amino acids on silica gel with cetyltrimethylammonium bromide - n-butanol - n-octane - water microemulsion. Detection by spraying with ninhydrin reagent. Investigation of the effects of the hydrous content of microemulsion and structures of amino acids on the hRf values. Several amino acid mixtures were separated and determined using a microemulsion with 40 % hydrous content, which was compared with the traditional mobile phase ethanol - water - acetic acid.

J. Liq. Chromatogr. Relat. Technol. 31, 611-618 (2008). TLC of eleven new bioactive 10-substituted 2,7-diazaphenothiazines on RP-18 with acetone and aqueous TRIS (tris-(hydroxymethyl)aminomethane) buffer pH 7.4 in a saturated chamber. The concentration of acetone in the mobile phase ranged from 50-85 % in 5 % increments. Evaluation under UV 254 nm and 366 nm. The method was used for the experimental determination of lipophilicity.

Anal. Chem. 75, 118-125 (2003). Online TLC separation and electrospray mass spectrometry (TLC/ESI-MS) by direct linking of a commercial overpressure TLC instrument, OPLC 50, and a Q-TOF mass spectrometer. Separation on silica gel with dichlormethane - methanol - water 60:35:8. A sensitivity of 5 pmol of glycosphingolipid was readily demonstrated for TLC/ESI-MS and 20 pmol for TLC/ESI-MS/MS production scanning to derive the saccharide sequence and long chain base/fatty acid composition of the ceramide. Initial preconditioning of TLC plates is necessary to achieve high sensitivity detection by reducing chemical background noise. Plates can be used repeatedly (at least 10 times) for analysis, although this may result in a minor reduction in TLC resolution. Following solvent development, separated components on the TLC plates can be detected in the conventional way by nondestructive staining or UV absorption or fluorescence and can be stored for on-line TLC/ESI-MS analysis at a later stage without reduction in mass spectrometric detection sensitivity and chromatographic resolution. Aspects for further improvement of OPLC instrumentation include use of narrower TLC plate dimensions and refined design of the eluate exit system.

J. Planar Chromatogr. 22, 35-42 (2009). HPTLC of phospholipids (lyso-phosphatidylcholine, sphingomyelin, phosphatidylcholin, lyso-phosphatidylethanolamine, phosphatidylinositol, phosphatidylethanolamine, phosphatidylserine, phosphatidic acid, and triacylglycerol) on silica gel with chloroform - ethanol - water - triethylamine 5:5:1:5. Detection by spraying with a solution of primulin (Direct Yellow 59). Evaluation under UV 366 nm. Detection by MALDI-TOF MS directly on the plates.

J. Planar Chromatogr. 22, 77-82 (2009). Detection of carbamates (carbaryl, propoxur, and carbosulfan) on silica gel activated at 110 °C for 5 min in the oven. After application the pesticide solutions were hydrolyzed by addition of 2 µL of 2 M sodium hydroxide solution. After 5 min, 5 µL diazotized p-aminoacetanilid was applied onto the spots to form the colored derivatives - red, yellow, and yellow-orange for carbaryl, propoxur, and carbosulfan, respectively. The plates were then placed horizontally in an oven at 110 °C and the colored derivatives migrated as concentric rings under the influence of the temperature gradient.