Chinese J. Trad. Pat. Med. (Zhongchengyao) 27 (2), 225-227 (2004). TLC on silica gel with 1) petroleum ether (30 - 60 ºC) - ethyl acetate 9:1; 2) benzene - glacial acetic acid 4:1. Detection 1) by spraying with 1 % vanillin solution and heating at 105 ºC for 10 min; 2) under UV 365 nm. Identification by fingerprint technique. Quantification of ferulic acid by densitometry at 325 nm. Validation of the method by investigation of linearity (0.16 µg - 1.6 µg, r = 0.9992); precision (RSD = 1.8 %, n= 5 within plate and RSD = 2.3 % plate to plate); reproducibility of five time assay towards the same sample (RSD = 0.5 %); and standard addition recovery (97.17 %, RSD = 0.9 %, n = 5). The results for three real life samples are given.

J. Chromatogr. A 1077 (2), 188-194 (2005). HPTLC of (+)-catechin (C), (-)-epicatechin (EC), (-)-gallocatechin (GC), (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECg), (-)-epigallocatechin gallate (EGCg), procyanidin B1, and procyanidin B2 on cellulose prewashed with water (not necessary, when water was used as developing solvent) and dried with a hair dryer, with 1) water; 2) 1-propanol - water 1:4; 3) 1-propanol - water - acetic acid 4:2:1; 4) 1-propanol - water - acetic acid 2:8:1 in horizontal developing chamber (sandwich configuration). Detection with vanillin - H3PO4 reagent. Water enabled the separation of epimers C from EC and GC from EGC, as well as the dimers procyanidin B1 and B2. Additionally C, EGC, B1 and B2 were separated from all the other compounds. The best separation of the five main catechins (EC, GC, EGC, ECg, EGCg) present in green tea extract was achieved using 1-propanol - water - acetic acid 2:8:1. The chromatograms of oak bark extract developed in solvents with higher water content (1-propanol - water 1:4 and 1-propanol - water - acetic acid 2:8:1) showed less bands than chromatograms developed in solvents with higher organic modifier content (e.g. 1-propanol - water - acetic acid 4:2:1). It was proved that such behavior was due to the presence of procyanidins beside the main component catechin.

J. Planar Chromatogr. 18, 85-88 (2005). 11 HPTLC of fenoxaprop-p-ethyl and degradation products on silica gel (prewashed with methanol - chloroform 1:1 and activated at 110 °C for 30 min) in a twin-trough chamber with toluene - dichloromethane 7:3. Visualization on irradiation with an UV lamp at 236 nm. Quantitative determination by absorbance measurement at 236 nm.

J. Planar Chromatogr. 18, 450-454 (2005). TLC of twenty-seven 2-benzylsulfanybenzothiazole derivatives on silanized silica gel in a pre-equilibrated normal chamber with 0.05 M phosphate buffer (pH 7.4 or 3.0) and methanol as organic modifier. Detection under UV light at 254 nm.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27(4), 483-485 (2005). TLC of tanshinone llA extracted from the title Chinese traditional patent medicine on silica gel with benzene - ethyl acetate 19:1. Quantitative determination by densitometry at 470 nm. Also determination of the compound by HPLC.

Planta Med. 70, 834-840 (2004). Analytical and preparative TLC of flavonoids (lavandulifolioside, plantamajoside, acteoside, leucosceptoside, and martynoside) on silica gel, RP18, polyamide and cellulose. E. g. 2D-TLC on cellulose with n-butanol - acetic acid - water 4:1:5 in the first and acetic acid - water 15:85 in the second direction, preparatice TLC on polyamide with ethyl acetate - ethanol - water 20:3:2 (2 x), 50:3:10, and 25:3:3 (4 x). Detection under UV light at 366 nm before and after spraying with 0.1 % diphenylboric acid 2-aminoethylester (natural products reagent) or 1 % aluminium chloride solution in ethanol.

J. Planar Chromatogr. 19, 104-108 (2006). HPTLC and TLC of ciprofloxacin and enrofloxacin on silica gel with dichloromethane - methanol - 2-propanol - 25% aqueous ammonia 3:3:5:2. The plate was developed in a DS chamber to the top and the separation chamber was then uncovered for about 1 cm to enable the solvent to evaporate. In this way the plate was developed continuously for 2 h. Bioautography by immersion of the plate in a microorganism solution (Bacillus subtilis), incubation for 22 h at 37 °C. Visualization by spraying with 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) solution and leaving for approximately 30 min at room temperature.

Planta Med. 71, 194-196 (2005). Analytical TLC of glaucopines A and B on silica gel with dichloromethane - methanol 19:1. Detection by spraying with 50 % sulfuric acid.