Chinese J. Trad. Patent Med. (Zhongchengyao) 26 (8), Appendix 16-18 (2004). Study of the identification of Shenbao capsules by TLC, in addition to microscopy and chemical methods. TLC of Shenbao capsule extracts on silica gel with 1) the lower phase of chloroform - ethyl acetate - methanol - water 15:40:22:10; 2) toluene - methanol 4:1. Detection 1) by spraying with 10 % H2SO4 in ethanol followed by heating at 105 ºC; 2) under UV light. Identification by fingerprint technique. Combination of the procedure with microscopy and chemical methods allows identification of all drug ingredients.

J. Planar Chromatogr. 17, 360-364 (2004). Analytical OPLC of ascorbigen (ASC, 2-C-[(indol-3-yl)methyl]-alpha-l-threo-l-glycero-3-hexulofuranosonic acid lactone) on silica gel by means of two-step development: the first step (n-hexane) served for elimination of the total wetness front, the second (chloroform - methanol 9:1) for the separation. Detection by spraying with 10 mL Procházka’s reagent (reaction with formaldehyde), then heated for 5 min at 105 °C. Quantitative determination by densitometry at 460 nm.

Chinese J. Trad. Pat. Med. (Zhongchengyao) 27(2), 246-248 (2005). TLC of extracts prepared by different processing technology on silica gel with 1) petroleum ether (60 - 90ºC) - chloroform - methanol 10:3:2; 2) chloroform - methanol 5:1; 3) benzene - glacial acetic acid 4:1. Detection 1) under UV 254 nm; 2) by spraying with 1 % vanillin - H2SO4 solution and heating at 105 ºC. Identification by fingerprint technique. Quantification of gastrodine by HPLC. Discussion of using the procedures for monitoring the preparation process and the quality control of the medicine products.

Chinese J. Trad. Pat. Med. (Zhongchengyao) 27(1), 34-37 (2005). TLC on silica gel with 1) benzene - acetone - ethyl acetate - ammonia water 10:15:20:1; 2) ethyl acetate - butanone - formic acid - water 5:3:1:1; 3) n-butanol - glacial acetic acid - water 7:1:2. Detection 1) by spraying with potassium iodobismuthate solution; 2) by spraying with 2 % FeCI3 in ethanol; 3) by spraying with vanillin - H2SO4 solution and heating; 4) under UV light. Identification by fingerprint technique. Quantification of geniposide by HPLC. The results for ten real life samples are given.

J. Planar Chromatogr. 18, 167-170 (2005). TLC and HPTLC of saponins from 70 species of Acer on silica gel in a horizontal chamber with chloroform - methanol - formic acid - water 200:80:20:19. Detection by spraying with 10 % sulfuric acid in ethanol or anisaldehyde - sulfuric acid reagent, followed by heating at 110 °C for 5 min, and evaluation in visible and UV light at 366 nm. Detection also by spraying with water or blood reagent.

J. Planar Chromatogr. 18, 164-166 (2005). TLC of non-steroidal anti-inflammatory drugs (NSAID), i.e. mefenamic acid, naproxen, ibuprofen, flurbiprofen, ketoprofen, paracetamol, and diclofenac, on silica gel with chloroform - methanol , chloroform - ethyl acetate, acetonitrile - methanol - water, acetonitril - methanol in different proportions. Detection with iodine vapor.

J. Planar Chromatogr. 18, 432-436 (2005). TLC of nine benzimidazole and benztriazole derivatives on silica gel impregnated with paraffin oil with methanol - water mixtures. After development the plates were dried and examined under UV light at 254 nm. A quantitative structure-retention relationship (QSRR) correlation study was performed.

J. AOAC Int. 88, 1562-1567 (2005). HPTLC of beta-asarone (cis-2,4,5-trimethoxy-1-propenylbenzene) and alpha-asarone in Calamus rhizome on caffeine-impregnated silica gel (prepared by immmersion of conventional silica gel plates into a solution of 80 g/L caffeine in dichloromethane for 1 s followed by drying at room temperature for 5 min, then heating at 80 °C for 5 min) with toluene - ethyl acetate 93:7. Quantitative determination by absorbance measurement at 313 nm. The method was validated in terms of stability of sample during chromatography, specificity for beta-asarone, linearity (25-300 ng, including samples containing 0.05-0.7 % beta-asarone), accuracy and precision. Recovery was 100.0-100.8 %, limit of detection 6.4 ng, and limit of quantitation 12.7 ng. The method allows proper identification of Calami rhizoma raw material, and the specific, accurate, and precise quantification of beta-asarone and alpha-asarone