Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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      130 046
      Development of synergy-based combination for learning and memory using in vitro, in vivo and TLC-MS-bioautographic studies
      M. KHAN, V. SRIVASTAVA, M. KABIR, M. SAMAL, A. INSAF, M. IBRAHIM, S. ZAHIRUDDIN, S. AHMAD* (*Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India, sahmad_jh@yahoo.co.in)

      Front. Pharmacol. 12, 678611 (2021). HPTLC of withanolide S in Withania somnifera and myristicin in Myristica fragrans on silica gel with toluene - ethyl acetate - acetic acid 5:4:1. Detection of myristicin under UV 254 nm. Detection of withanolide S by spraying with 5 % anisaldehyde sulfuric acid, followed by visualization under UV 540 nm. Screening of anticholinesterase active metabolites by spraying with DTNB/ATCI reagent (1 mmol/L 5,5-dithiobis-(2- nitrobenzoic acid) and 1 mmol/L acetyl thiocholine iodide). 

      Classification: 13c
      130 047
      High‑performance thin‑layer chromatography in combination with an acetylcholinesterase‑inhibition bioassay with pre‑oxidation of organothiophosphates to determine neurotoxic effects in storm, waste, and surface water
      N. BAETZ, T. SCHMIDT, J. TUERK* (*Institute of Energy and Environmental Technology, Bliersheimer Str. 58 – 60, 47229 Duisburg, Germany, tuerk@iuta.de)

      Anal. Bioanal. Chem. 414, 4167-4178 (2022). HPTLC of organothiophosphates malathion, parathion, and chlorpyrifos in storm, waste, and surface water on silica gel with an eluent mixture of cyclohexane, dichloromethane, and acetone in different proportions and with increasing migration distances. The plate was sprayed with n-bromosuccinimide to oxidize the organothiophosphates. AChE‑inhibition assay was performed by spraying with an AChE solution, followed by incubation at 37 °C for 5 min. Plates were sprayed with the substrate indoxyl acetate freshly prepared in methanol, followed by incubation for 45 min at room temperature. Detection at 670 nm using the fluorescence mode. 

      Classification:
      130 048
      Coding recognition of the dose–effect interdependence of small biomolecules encrypted on paired chromatographic‑based microassay arrays
      Y. DENG* (Deng Yifeng), Z. LIN (Lin Zhenpeng), Y. CHENG (Cheng Yuan) (*Guangdong Key Laboratory for Research & Development of Natural Drugs, Guangdong Medical University, Zhanjiang 524023, China, dengyf@gdmu.edu.cn)

      Anal. Bioanal. Chem. 414, 5991-6001 (2022). 2D-HPTLC fingerprint of Alpinia officinarum on silica gel in 384-well microplate array format (4.5 × 4.5 mm) matrix with trichloromethane - methanol - petroleum ether 97:3:25 in the first dimension and ethyl acetate - petroleum ether - acetic acid 15:35:1 in the second dimension. The paired chromatographic-based microassay array with the consistent chromatographic distribution was prepared by transferring a portion of the sample from the stock chromatographic-based microassay arrays to the corresponding array units of another 384-well microplate. A G‑quadruplex ligand bioassay was used to evaluate the ligand activity of the components in each array unit of the chromatographic-based microassay array. Further analysis by mass spectrometry. 

      Classification: 4e
      130 023
      Quality standard of traditional Chinese medicines: comparison between European Pharmacopoeia and Chinese Pharmacopoeia and recent advances
      F. LEONG (Leong Fong), X. HUA (Hua Xue), M. WANG (Wang Mei), T. CHEN (Chen Tongkai), Y. SONG (Song Yuelin), P. TU (Tu Pengfei), X. CHEN (Chen Xiao-Jia)* (*State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao, China; xiaojiachen@um.edu.mo)

      Chinese Medicine 15, 76 (2020). This review compared the 2020 editions of Chinese (ChP) and European Pharmacopoeas (EuP) in different aspects of quality control of traditional Chinese medicinal plants (73 of which drugs were common to both, but with differences in species or organs for 17 of them). Discussed points included history, identification, plant origin and processing, sample preparation, marker selection, tests and assays, as well as advanced analytical techniques for quality control and for the establishment of comprehensive quality standard. TLC was discussed in relation to its following aspects: purposes, markers/references, techniques and result description.
      (A) The main uses of TLC and HPTLC were (1) chemical-based identification of the plant in a more accurate and precise method than by macroscopic and microscopic observation only, and in a more direct and easily interpretation than HPLC, and allowing the simultaneous analysis of multiple samples in parallel; (2) control of possible adulterants; (3) quantification of active compounds. Both uses (1) and (2) were combined in some EuP monographs: as example were given the roots of Angelica dahurica, A. pubescens, A. sinensis, using TLC for identification of the species and of adulterants from other species (Angelica, Levisticum and Ligusticum).
      (B) In ChP, identification through TLC was in most cases achieved by fingerprint comparison to an official reference extract or herb (herbal reference substance). At the opposite, EuP often indicated analytical markers, irrespective of any pharmacological activity, but chosen only for analytical purposes in TCM identification and quantification. Examples were: aescin and arbutin as analytical markers for TLC identification of Anemarrhena asphodeloides rhizome and Panax notoginseng root.
      For the TLC system suitability assessment tests, ChP used the same intensity markers or active markers that were chosen for the identification or assay; whereas EuP often used other specific references, e.g. isoeugenol and methyleugenol in the case of Ophiopogon japonicus roots.
      (C) For the techniques, conventional separations and chemical derivatizations were used. Hyphenations of TLC to other analytical methods (e.g. MS) were absent. Only one monograph applied an effect-directed analysis directly on TLC chromatogram (free DPPH• radical scavenging assay for TLC identification of Rehmannia glutinosa root, in ChP).
      Sometimes, the TLC methods were different between both reference books for the same species. Example was given for Belamcanda chinensis (=Iris domestica) rhizome: in EuP, development on silica gel with cyclohexane – ethyl acetate – acetic acid 20:80:1, detection under UV 254 nm, comparison to standards coumarin and irisflorentin; whereas in ChP, development on polyamide layer with chloroform – butanone – methanol (3:1:1), detection under UV 365nm after derivatization with aluminium chloride, comparison to a reference rhizome powder.
       (D) Finally, the results in ChP were described as a text stating the similarity of sample profile with the profile of the chosen reference, whereas the results in EuP were described with a schematic box indicating the positions of bands of interest.

      Classification: 1, 2a, 32e
      130 059
      Evaluation of the efficacy and safety of Unani formulations in Pityriasis versicolor: A randomized controlled trial
      B. ISMAIL, M. NAWAB*, S. FATIMA, A. MINHAJUDDIN, M. NAIKODI (*Department of Moalajat (Medicine), National Research Institute of Unani Medicine for Skin Disorders, Opp. ESI Hospital, Eragadda, Hyderabad, 500038, India, ccrumnawab@gmail.com)

      J. Ethnopharmacol. 289, 115035 (2022). HPTLC of Unani pharmacopoeial preparations Itrifal Hakim Ali (1) and Habb-e-Kalaf (2) on silica gel with toluene - ethyl acetate 9:1 for (1) and toluene - ethyl acetate 4:1. Detection under UV light at 254 and 366 nm. 

      Classification: 32e
      130 061
      Pharmacological studies of rhizomes of extract of Cyperus tegetum, emphasized on anticancer, anti-inflammatory and analgesic activity
      A. CHATTERJEE*, R. KHANRA, M. CHATTOPADHYAY, S. GHOSH, R. SAHU, G. NANDI, H. SEKHAR, P. CHAKRABORTY (*Bengal School of Technology, Chinsurah, Hooghly, West Bengal, India, acchatterjee@yahoo.co.in)

      J. Ethnopharmacol. 289, 115035 (2022). HPTLC of stigmasterol and some polyphenolic compounds in the rhizome of Cyperus tegetum on silica gel with chloroform - ethyl acetate - formic acid 5:4:1 and toluene - methanol 9:1. Detection by spraying with p-anisaldehyde reagent in a cold solution of methanol - glacial acetic acid - phuric acid 17:2:1, followed by heating at 100 - 105 °C for 5-10 min.

      Classification: 7
      130 063
      Comparative evaluation of phenolic profile and antioxidant activity of new sweet cherry (Prunus avium L.) genotypes in Turkey
      M. OCKUN*, Y. GERCEK, H. DEMIRSOY, L. DEMIRSOY, I. MACIT, G. OZ (*Mehmet Ali Ockun, Department of Biology, Botany Division, Istanbul University, Istanbul, Turkey, mehmetaliockun@hotmail.com)

      Phytochem. Anal. 33, 564-576 (2022). HPTLC of flavonoids (1), anthocyanin (2) and antioxidant structures in sweet cherry (Prunus avium) on silica gel with ethyl acetate - dichloromethane - formic acid - acetic acid - water 100:25:10:10:11 for (1) and ethyl acetate - formic acid - acetic acid - water 100:11:11:26 for (2). Detection by spraying with natural product reagent/polyethylene glycol 400 (NP/PEG) solution or 0.1 % methanolic DPPH solution. Qualitative analysis under UV light at 366 nm. 

      Classification: 8a
      130 065
      Bioautography-guided HPTLC–MS as a rapid hyphenated technique for the identification of antimicrobial compounds from selected South African Combretaceae species
      C. ANOKWURU, W. CHEN, S. VUUREN, S. COMBRICK, A. VILJOEN* (*Department of Pharmaceutical Sciences, Faculty of Science, Tshwane University of Technology, Private Bag X680, Pretoria, 0001, South Africa, viljoenam@tut.ac.za)

      Phytochem. Anal. 33, 1177-1189 (2022). HPTLC of 35 species from four genera
      (Combretum, Pteleopsis, Quisqualis, Terminalia) of Combretaceae on silica gel with ethyl acetate - formic acid - water 50:3:3. Detection by spraying with 10 % methanolic sulfuric acid and visualization under white light. Detection of flavonoids by spraying with Natural Product Reagent and examined under UV light at 366 nm. For bioautography analysis, the developed plate was sterilized under UV light for 1 h, then overlaid with tryptone soya agar containing the appropriate bacterial culture: Staphylococcus aureus (ATCC 25923), Bacillus cereus (ATCC 11778), Escherichia coli (ATCC 8739), and Salmonella typhimurium (ATCC 14028).
      Active compounds were identified by mass spectrometry. 

      Classification: 28a
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