J. Planar Chromatogr. 17, 54-57 (2004). TLC of food pigments (patent blue V, quinoline yellow, brilliant blue FCF, tartrazine, azorubine, ponceau 4 R, curcumine, indigo carmine, cochineal, methyl violet, mixed carotenes, plain caramel, erythrosine B, orange yellow S) and artificial sweeteners (aspartame, acesulfame, sodium cyclamine) and benzoic acid on silica gel. Pigments were developed with isopropanol - 12.5 % ammonia 5:1; sweeteners and benzoic acid were developed with ethanol - isopropanol - 12.5 % ammonia 10:40:1. Derivatization with a solution of 0.04 g bromocresol green in 100 mL ethanol containing 0.1 M sodium hydroxide.

Chinese J. Trad. Patent Med. (Zhongchengyao) 26 (3), 192-195 (2004). TLC of Qufeng medicinal wine on silica gel with 1) n-hexane - ethyl acetate 9:1; 2) ethyl acetate - methanol - water 100:17:13; 3) toluene - ethyl acetate - formic acid - water 20:10:1:1; 4) chloroform - methanol - water 40:10:1; 5) n-hexane - ethyl acetate - chloroform - glacial acetic acid 50:15:5:1. Detection 1) under UV 365 nm; 2) by spraying with 1 % AlCl3 in methanol and under UV 365 nm; 3) by spraying with 5 % H2SO4 in ethanol and heating at 105 ºC; 4) by exposing to ammonia vapor. Identification by fingerprint technique. Quantification of emodin by densitometry at 460 nm. Validation of the method by investigation of its linearity range (0.133-1.330 µg/spot, r=0.9999), precision (RSD=1.44 %, n=6), recovery (99.24 %, RSD=0.74 %, n=12).

Chinese J. Trad. Patent Med. (Zhongchengyao) 26 (5), 374-376 (2004). TLC of Qingdai powder extract on silica gel with 1) benzene - chloroform - acetone 5:4:1; 2) petroleum ether (60-90 ºC) - benzene - ethyl acetate 9:2:1; 3) benzene - ethyl acetate - isopropanol - methanol - water 60:30:10:50:15:3; 4) petroleum ether (30-60 ºC) - benzene - ethyl acetate - glacial acetic acid 20:40:14:1. Detection 1) under day light; 2) by spraying with 5 % vanillin-H2SO4 solution and heating at 105 ºC ; 3) under UV 365 nm; 4) under UV 254 nm. Identification by fingerprint techniques. Quantification of indigo by HPLC with method validation.

J. Planar Chromatogr. 17, 459-463 (2004). TLC of standard solutions of nine flavonoids and six phenolic acids (cinnamic, o-coumaric, m-coumaric, p-coumaric, caffeic, ferulic acid, galangin, quercetin, pinocembrin, naringenin, apigenin, chrysin, kaempferol, morin, acacetin) on silica gel in pre-saturated developing chambers with 1) n-hexane - ethyl acetate - glacial acetic acid 31:14:5, or 2) chloroform - methanol - formic acid 88:7:5. After drying, bands were visualized under short- and long-wavelenghth UV light. Detection by spraying with 1 % aluminium trichloride solution and evaluation under long-wavelength UV light. Standards were chromatographed again with a propolis extract. First, plates were developed with mobile phase 1 (or 2), the eluent was evaporated, standard solutions were applied again, and the plate was rotated through 90 ° and chromatographed again with mobile phase 2 (or 1). The presence (or absence) of all standards was determined according to their Rf values and fluorescence colors. Quantitative determination by absorbance measurement at 254 and 366 nm.

J. Planar Chromatogr. 17, 342-349 (2004). TLC of 29 1,2,3,4-tetraoxanes on silica gel, cyano phase, and RP-18. The binary mobile phases ethyl acetate - petroleum ether and ethyl acetate - toluene were used under normal-phase conditions, and water - organic modifier (methanol, acetone, dioxane) under reversed-phase conditions. Chromatography was performed using a HPTLC horizontal developing chamber equilibrated for 15 min with the vapor of the mobile phase. Detection by spraying with 50 % sulfuric acid and heating until the spots became visible.

Part I. J. Planar Chromatogr. 17, 275-279 (2004). HPTLC of nicotinic acid and selected derivatives (methyl nicotinate, ethyl nicotinate, isopropyl nicotinate, butyl nicotinate, hexyl nicotinate, benzyl nicotinate, nicotinamide, N-methyl nicotinamide) on RP-18 with methanol - water in different volume proportions after chamber saturation for 30 min. Detection under UV light at 254 nm. Investigation of the lipophilicity by TLC and use of the data for quantitative structure-activity relationships.

J. Planar Chromatogr. 18, 141-146 (2005). TLC of fatty acids (ethanoic, propanoic, butanoic, pentanoic, hexanoic, heptanoic, and octanoic acid) on silica gel with hexane - acetone 4:1 or acetone - water - chloroform - ethanol - aqueous ammonia 30:1:3:5:11 with chamber saturation for 30 min. Only bromocresol green, bromophenol blue, potassium permanganate, and methyl red can be used for the detection. Among the new visualizing agents dipping of the plates in an aqueous solution of alkaline blue enables the detection of the free fatty acids from propanoic to octanoic. Dipping generated better contrast regarding the spots than spraying.

J. Planar Chromatogr. 18, 465-470 (2005). HPTLC of cholic, glycocholic, glycodeoxycholic, chenodeoxycholic, deoxycholic, lithocholic, and glycolithocholic acid on RP-18 W, RP-2, and cyano phases in a presaturated chamber with mixtures of an organic modifier (methanol, dioxane, acetonitrile, acetone) and water in different volume proportions which were varied in steps of 5 % from 35 to 80 %. Detection by spraying with a 10 % aqueous solution of sulfuric acid or by dipping in a 10 % solution of phosphomolybdic acid in ethanol and heating at 120 °C for 20 min. Investigation of relationships between lipophilicity obtained by use of RP-HPTLC, experimental and theoretical partition coefficients, and selected structural descriptors.