Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (9), 716-719 (2004). TLC on silica gel with 1) ethyl acetate - chloroform - formic acid 3:2:1; 2) chloroform - methanol 7:2; 3) petroleum ether (60-90 ºC) - ethyl acetate 7:3. Detection 1) under UV 365 nm; 2) by spraying with 10 % H2SO4 in ethanol and heating at 105 ºC for 5 min; 3) by spraying with vanillin - H2SO4 solution. Identification by fingerprint technique. Quantification of icarrin by HPLC.

Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (9), App.14-16 (2004). TLC on silica gel with 1) toluene - ethyl acetate - formic acid 25:20:4; 2) ethyl acetate - methanol - water 100:17:13. Detection 1) by spraying with diazotized para-nitroaniline solution; 2) by spraying with 5 % AlCl3 in ethanol and under UV 365 nm. Identification by fingerprint technique. Quantification of hesperidin by HPLC.

Part IV. Separation on RP 18 plates with ternary mobile phases. J. Planar Chromatogr. 18, 228-233 (2005). HPTLC of heptanoic to eicosanoic acids on RP-18 (with and without concentrating zone). The best chromatographic conditions for separation of the fatty acids were RP-18 plates without concentrating zone and methanol - ethanol - water 9:9:2, and RP 18 plates with concentrating zone and methanol - ethanol - water or methanol -n-propanol - water 9:9:2. Detection by exposure to iodine vapour. Separation of acids from methanoic to butanoic and from tetracosanoic to triacontanoic acid was not possible.

Juss) and commercial neem based formulations using HPTLC and extended length packed-columns SFC method. Chromatographia 62 (3-4), 183-195 (2005). Two chromatographic techniques are described for the separation and quantitative determination of azadirachtin A and B, salannin, and nimbin present in the crude extract of neem seed kernels and commercial neem based formulations. HPTLC separation of markers on silica gel with ethyl acetate - benzene 7:3. Visualization under UV 254 nm. The other technique was based on extended length packed column supercritical fluid chromatographic (PC-SFC) separation of the markers. Validation of both methods in terms of precision, robustness, recovery, limits of detection and quantitation. The analysis of variance (ANOVA) and Student’s t-test were applied to correlate the results of quantitative determination of markers by means of HPTLC and PC-SFC method.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27 (3), 279-282 (2005). TLC of the extracts of the title Chinese traditional patent medicine on silica gel with 1) ethyl acetate - methanol - ammonia 17:2:1; 2) n-butanol - glacial acetic acid - water 19:5:5. Detection 1) under UV 365 nm; 2) by spraying with ninhydrin solution followed by heating at 105 ºC until the spots are visualized. Identification by fingerprint technique. Quantification of paracetamol and pseudoephedrine arginine by HPLC.

J. Planar Chromatogr. 18, 336-343 (2005). HPTLC of thirty-three compounds with benzodiazepine properties on silica gel after prewashing first with methanol and then with dichloromethane - methanol 19:1 in a saturated horizontal chamber and three optimized mobile phases: dichloromethane - methanol 19:1, ethyl acetate - cyclohexane - 25 % ammonia 50:40:0.1, and in a third run cyclohexane - acetone - methyl t-butyl ether 3:2:1. Diode-array HPTLC makes it possible to identify all the compounds with high certainty down to a level of 20 ng. An algorithm for spectral recognition which is combined with Rf values from the three separation steps into one fit factor is presented. This set of data is unique for each of the compounds investigated and enables unequivocal identification.

J. Planar Chromatogr. 19, 250-252 (2006). TLC of piperine and chavicine on silica gel with heptane - ethyl acetate 3:2 in unsaturated chambers. Detection under UV light at 254 nm.

J. Planar Chromatogr. 19, 85-86 (2006). TLC of methomyl on silica gel with benzene - ethyl acetate 3:2 in a presaturated TLC chamber. After drying the plate was sprayed with 1 % phloroglucinol solution then with 50 % hydrochloric acid. The plate was heated at 100 °C for 5 min. Detection limit of the pink-violet spot was 5 µg.