J. Planar Chromatogr. 19, 146-150 (2006). Comparison of the chromatographic behavior of twelve long-alkyl-chain derivatives of tetraphenylporphyrines on alumina and silica gel normal and reversed-phase TLC using several different organic mobile phases. TLC of 12 derivatives of tetraphenylporphyrins on alumina with dichloromethane - cyclohexane 3:7 and 7:13, on silica gel with chloroform - pentane 13:27, chloroform - hexane 9:11, dichloromethane - hexane 11:9, chloroform - cyclohexane 19:21, and dichloromethane - cyclohexane 11:9, and on RP18 with 7 mobile phases. Visual detection of spots.

Planta Med. 72, 351-357 (2006). Analytical and preparative TLC of ergosta-4,6,8(14),22-tetraen-3-one, beta-sitosterone, 2,6,11-trimethyldodeca-2,6-10triene, and stigma-4-ene-3,6-dione on silica gel with n-hexane - ethyl acetate 6:1. Preparative TLC of tsangin A with dichloromethane - acetone 25:1; of tsangin B and beilschmien C with dichloromethane - acetone 20:1; of beilschmien A and B with n-hexane - ethylacetate 2:1. Detection under UV light at 254 nm.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27 (7), 854-856 (2005). TLC of chenodeoxycholic acid in Hedan tablets on silica gel with n-hexane - ethyl acetate - acetic acid - methanol 20:25:2:3. Detection by spraying with 10 % H2SO4 in ethanol followed by heating at 105 ºC until the spots are visualized. Quantification of chenodeoxycholic acid by densitometry at 375 nm. Validation of the procedure by investigation of its linearity range (0.47 - 2.33 µg/spot, R = 0.9992); of its repeatability (3.3 %, n = 5); of its precision (3.9 %, n = 5 within plate and 4.7 % n= 5 plate-to-plate); and its standard addition recovery (98.4%, RSD = 2.5 %, n = 5).

Benth, identification by TLC fingerprint. Indian Drugs 42 (6), 392-395 (2005). Dried leaves of Pithecellobium Dulce. Benth were successively extracted with n-hexane, chloroform and alcohol. Each extract was evaluated for antimycobacterial activity. These extracts were subjected to TLC fingerprint profile for identification on silica gel with chloroform - methanol 9:1.

Chromatographia 62 (11-12), 653-657 (2005). TLC of S(+) and R(-) ibuprofen on RP phase with beta-cyclodextrin - methanol 15:1. The UV densitometric detection was carried out at 222 nm. Limit of detection for S(+) and R(-) ibuprofen is 1 µg/mg. Precision and repeatability are good, the obtained results are within the range x mean ± 2. Recovery for both isomers is approximately 99 % and linearity was found to be in the range of 0.01-0.3 %. The presence of both isomers S(+) and R(-) ibuprofen was observed in all preparations at comparable concentrations from 56-66% for S(+) isomer and from 34-44 % for R(-) isomer.

Acta Chrom. 11, 62-74 (2001). TLC of basic isolates of Chelidonium majus L. (homochelidonine, allocryptopine, chelidonine, protopine, chelerythrine, san-guinarine and berberine) on silica gel with different mobile phases: toluene – ethyl acetate – methanol 17:2:1, toluene – ethyl acetate 4:1, toluene – ethyl acetate – methanol 11:8:1 and toluene – methanol 19:1; and on aluminum oxide 60 type E (basic) with toluene – ethyl acetate 9:1, 4:1, and 3:2; and toluene – methanol 19:1 in horizontal chamber. Evaluation under UV light at 254 and 366 nm, or by spraying with Dragendorff’s reagent. The HPLC-UV method was developed in parallel.

J. Planar Chromatogr. 19, 398-400 (2006). HPTLC of curcumin, piperine, and thymol on silica gel, pre-washed with methanol, without chamber saturation with toluene - ethyl acetate - methanol 18:2:1. Quantitative determination by absorbance measurement at 420 nm for curcumin, 333 nm for piperine, and 277 nm for thymol. Limit of detection for curcumin was 25 ng, for piperine 5 ng, and for thymol 50 ng. Rapid identification of the three compounds is also possible by spraying the plate with anisaldehyde in sulfuric acid reagent.

Simultaneous separation of mixtures of zinc, nickel, mercury and cadmium or manganese cations. Acta Chrom. 12, 177-188 (2002). The effect of surfactant concentrations below and above the critical micellar concentration on the retention behaviour of metal ions was examined. The effect of organic and inorganic additives on the mobility and separation efficiency of the metal ions was also assessed. TLC of mixtures of Zn2+, Ni2+, Hg2+, and Cd2+ or Mn2+ on silica gel with 50 mM aqueous micellar solution of N,N-cetyltrimethyl-ammonium bromide (CTAB). Detection of Ni2+ with a 1 % solution of alcoholic dimethylglyoxime, of Zn2+,Cd2+, and Hg2+ with a 0.5 % solution of dithizone in CCl4, and of Mn2+ with a 1:1 mixture of 2 M sodium hydroxide and 30 % H2O2. The proposed method was used for separation and identification of cations from drag-out nickel-plating solution and a sludge sample containing their hydroxides, as well as for semi-quantitative Ni2+-determination.