Indian Drugs 41 (3), 160-164 (2004). Combinations of ofloxacin and tinidazole (1:3) are available as tablet dosage forms. Stability indicating HPTLC of ofloxacin and tinidazole on silica gel with dioxan - ethyl acetate - toluene - acetic acid - water 5.5:5:3.2:2:2 . Quantification by absorbance measurement at 307 nm. The Rf value of ofloxacin and tinidazole was found to be 0.12 and 0.85 respectively. Recovery was 99.85 % and 99.45 % for ofloxacin and tinidazole respectively. The proposed method was quantitatively evaluated in terms of calibration of concentration range, stability, and accuracy. It can be adopted for routine analysis of the formulation.

J. AOAC Int. 87, 573- 578 (2004). TLC of propanolol/reagent azo adduct (with diazotized 4-amino-3,5-dinitrobenzoic acid) on silica gel with ethyl acetate - methanol 9:1, chloroform - methanol 4:1, methanol - ammonia 200:3 (plate was impregnated with 0.1 M methanolic KOH before spotting). Evaluation under UV light at 254 nm.

J. Planar Chromatogr. 17, 207-212 (2004). HPTLC of malvidin 3-glucoside, cyanidin 3-glucoside, delphinidin 3-glucoside, peonidin 3-glucoside,and petunidin 3-glucoside on silica gel with ethyl acetate - formic acid - twice distilled water 17:2:3 in an unsaturated twin trough chamber. After drying detection with methanolic 2,2-diphenyl-1-picrylhydrazyl reagent. Quantitative determination by videodensitometry.

J. Planar Chromatogr. 17, 192-199 (2004). TLC of 3-ethyloxycarbonyl-5-substituted-2,4-dioxotetrahydro-1,3-thiazole derivatives (2’-fluoro-6’-chlorobenzylidene, 1’-naphthylidene, 4’-methoxybenzylidene, 2’-oxybenzylidene, 2’-thienylidene, 4’-dimethylaminobenzylidene, 1’-carboethoxy-3’-indolylidene, 3’,4’-dimethoxybenzylidene, 4’-isopropylbenzylidene, benzylidene) on silica gel with non-polar diluent (hexane) - polar modifier (ethyl acetate, acetone, or dioxane) and on reversed-phase systems of the type rice starch with polar diluent (aqueous ammonia) - polar modifier (methanol, acetone, or dioxane). Examination of the dried plates after development under UV light at 254 nm.

Chinese J. Trad. Pat. Med. (Zhongchengyao) 27(2), 158-161 (2005). HPTLC on silica gel with 1) cyclo-hexane - ethyl acetate - methanol 4:5:1; 2) n-hexane - ethyl acetate 3:1; 3) n-hexane - ethyl acetate 9:1; 4) cyclohexane - ethyl acetate - diethylamine 45:20:3. Detection 1) under UV 365 nm; 2) by spraying with 10 % H2SO4 in ethanol and heating; 3) by spraying with diluted potassium iodobismuthate solution followed by spraying with sodium nitrite solution in ethanol. Identification by fingerprint technique. Semi-quantitative determination of aconitine by comparison with the standard. Quantification of strychnine by HPLC. The results for some real life samples are given.

J. Planar Chromatogr. 18, 344-348 (2005). TLC of beta-diketonato complexes of the type M(acac) 3-n(phacphac)n and M(acac)3-n(phSacphSac)n, where M represents cobalt(III) or chromium(III), acac is the pentanedionato ion, phacphac the 1,3-diphenyl-1,3-propanedionato ion, and phSacphSac the 3-mercato-1,3-diphenylprop-2-en-1-thione ion (n= 0-3), on RP-18 with mixtures of tetrahydrofuran, acetonitrile, or acetone (as organic modifier) with water.

J. Planar Chromatogr. 18, 176-180 (2005). OPLC separation of alkaloids on silica gel using different mobile-phase velocities from 100 to 400 µL/min and investigation of this effect on properties such as retardation factors, reproducibility, efficiency, number of theoretical plates, HETP, and resolution. OPLC of tertiary alkaloid standards (allocryptopine, protopine, chelidonine) and quaternary alkaloid standards (chelerythrine, chelilutine, sanguinarine, and chelirubine) on silica gel with toluene - ethyl acetate - methanol 14:3:3 for tertiary alkaloids and toluene - ethyl acetate - methanol 83:15:2 for quaternary alkaloids.

J. Planar Chromatogr. 18, 319-322 (2005). HPTLC of emodin and phenolic acids (protocatechuic, homoprotocatechuic, caffeic, syringic, vanillic, ferulic, p-hydroxyphenylacetic, alpha-resorcylic, p-coumaric, gallic and ellagic acid) on silica gel in horizontal chambers with toluene - dichloromethane - ethyl acetate 4:4:1. Also two-dimensional TLC of phenolic acids on cellulose with benzene - methanol - acetic acid - acetonitrile 16:2:1:1 in the first direction and sodium formate - formic acid - water 10:1:200 in the second direction. After drying the chromatograms were observed under UV light at 254 nm before and after treatment with ammonia vapor. Derivatization was performed by spraying with either diazotized sulfanilic acid in 20 % sodium carbonate solution or with 2 % aqueous iron(III) chloride. Detection limits between 10 and 64 ng. Videodocumentation and quantitation by densitometry.