J. Sep. Sci. 38, 2737-2745 (2015). 2D-HPTLC of malachite green, methyl red, p-amino-azo-benzene and rhodamine on a dual-phase monolithic layer with ethyl acetate - ethanol - water 5:4:9. Detection of the separated dyes was performed with surface-enhanced Raman spectroscopy. The dual-phase of hydrolyzed glycidyl methacrylate-ethylene dimethacrylate and butyl methacrylate-ethylene dimethacrylate monolithic porous polymer with a slant boundary was prepared by two-step polymerization method allowing the separation of low-molecular weight dyes.

J. Planar Chromatogr. 28, 248-250 (2015). TLC of phenylalanine, tyrosine and tryptophan on silica gel prepared by adding different concentrations of vancomycin with n-butanol - methanol - water - acetic acid 25:8:6:2. The best separations of the three racemic amino acids were achieved with more than 1.86 g of vancomycin in 25 g of silica gel. Vancomycin proved to be sufficiently stable and showed good enantioselectivity.

PLoS ONE 9(1), e1003109 (2013). In order to understand how Borrelia burgdorferi acquires cholesterol from its host, five experiments were performed. HPTLC on silica gel with chloroform – methanol 17:3, the lipids were extracted through the Bligh and Dyer method: (1) from the spirochete incubated 4 h in a medium containing 4 mg/L BODIPY-cholesterol (fluorescing only in hydrophobic environment), (2) from the spirochete incubated 2 h in a medium containing 10 µCi/L tritiated cholesterol, (3) from HeLa cells incubated with the spirochete itself charged with tritiated cholesterol (after removal of all spirochetes). The same analysis was applied (4) to the purified outer membrane vesicles of the spirochete previously charged with BODIPY-cholesterol. In cases (1) and (4), UV detection showed that the BODIPY-cholesterol is incorporated into the cholesterol glycolipids of B. burgdorferi, whereas without incubation it has the same hRf as cholesterol. In cases (1), (2) and (4), iodine staining made free cholesterol and cholesterol-glycolipids (galactopyranosides) visible, but no other (cholesterol-free) lipids. In case (2), the chromatogram was sprayed with EN3HANCE Spray (DuPont), exposed using BioMax MR Film (Kodak) for 4 and 14 days at −80 °C and developed using a Medical Film Processor Model SRX-101A (Konica); the radiolabelled cholesterol was shown incorporated into the cholesterol-glycolipids. In cases (2) and (3), the radiolabelled zones on the layer were scraped off and analyzed by liquid scintillation counting; cholesterol-glycolipids were found (more than free cholesterol) transferred from the spirochete to the HeLa plasmalemma.

Food Control. 59, 700-707 (2016). HPTLC of aflatoxin B1 conjugated with carboxymethoxylamine hemihydrochloride (aflatoxin B1-CMO) on silica gel with chloroform - methanol 9:1 and 15 % acetic acid. Identification under UV light at 366 nm. The method was applied for conjugation with bovine serum albumin.

J. Liq. Chromatogr. Relat. Technol. 39, 549-557 (2016). Review of the use of TLC and HPTLC for the analysis of inks in forensic applications, including sample preparation, layers, sample application, detection, documentation and results interpretation. The author also described the application of TLC in food dye analysis as well as future trends in the field.

J. Planar Chromatogr. 29, 299-305 (2016). Analysis of the antioxidants ascorbic acid (1), gallic acid (2), caffeic acid (3), quercetin (4), and biogenic amines (–)-epinephrine (5), (–)-norepinephrine (6), isoprenaline hydrochloride (7), dopamine hydrochloride (8), 3,4-dihydroxy-d-phenyl-alanine (d-dopa) (9), and 3′,4′-dihydroxy-2-(methylamino)acetophenone hydrochloride (adrenalone) (10) applied as individual zones in different concentrations on silica gel, without development. Radical-scavenging activity was measured by spraying with 0.03 % 2,2-diphenyl-1-picrylhydrazyl (DPPH radical reagent) solution in methanol and stored in dark at 22 °C for 5 min, followed by scanning in a dark place. Image data was converted into chromatograms, from which the spot area as a function of the reaction time was monitored. The title "Chromatographic approach" is misleading, as there is no chromatography.

CBS 114, 5-7 (2015). HPTLC of biodiesel (B100), biodiesel blended with diesel fuel in different proportions (BX, with X_x000D_ being the volume percent of B100 in the mixture) and standard monoacylglyceride on silica gel with the AMD 2 system in a 3-step development starting with 100 % t-butyl methyl ether (up to 40 mm), followed by dichloromethane – n-heptane 4:1 up to_x000D_ 60 mm and then 3:2 up to 90 mm as final migration distance. Detection by dipping in a 0.02 % methanolic solution of primuline. Quantitative determination of monoacylglycerides via the 1-oleyl glycerol standard by fluorescence measurement at 366/>400 nm. Elution of target zones with methanol, flow rate 0.2 mL/_x000D_min, via the TLC-MS Interface (oval elution head 4 x 2 mm) into an ion-trap MS operating in positive ESI mode. The final chromatogram showed a migration distance of 15-20 mm for the monoacylglyceride of BX, other lipids detected were diacylglycerides,_x000D_ triacylglycerides, fatty acids (20–60 mm) and fatty acid methyl esters (60–83 mm), the preponderant chemical class. The sample peak at 86 mm corresponded to the diesel component in the blend.

Planta Medica 82(11/12), 1009-1015 (2016). _x000D_
To check the purity of six alkaloids isolated from fractions of a methanolic extract of Carapichea ipecacuanha, TLC on silica gel with dichloromethane – methanol – 25 % ammonia 186:13:1, detection with Dragendorffʼs reagent. By TLC comparison with standards (and confirmation by HPLC-MS), emetine (found to be a strong hERG potassium channel blocker) and N-methylemetine were identified._x000D_