CBS 116, 9-10 (2016). HPTLC of herbal slimming drugs and the standard orlistat on silica gel with toluene – ethyl acetate 4:1 with chamber saturation (with filter paper) to the migration distance of 70 mm. Detection by dipping in phosphomolybdic acid reagent (5 g in 100 mL ethanol) and heating at 110 °C for 5 min. Evaluation under UV 254 nm, 366 nm and white light. Quantitative determination by absorbance measurement at 195 nm before derivatization to detect illegally added orlistat in the herbal drugs. The LOD of orlistat standard was 70 ng/band.

J. Planar Chromatogr. 29, 361-365 (2016). Analysis of phenols in bio-oil from agricultural wastes obtained during fractionation. Column chromatography (CC) on silica gel in a glass column (i.d. 1 cm, 50 cm length) filled with n-hexane. The concentrated organic phase was mixed with silica gel 1:1, loaded onto the column and successively eluted with dichloromethane – acetone 20:1, ethyl acetate, and methanol. 24 eluate fractions were obtained during the fractionation using CC, 12 with dichloromethane – acetone mixture, 5 with ethyl acetate, and 7 with methanol. Phenolic compounds were present only in the dichloromethane – acetone mixture fraction which was therefore selected as the proper eluent to obtain all the phenolic fractions dissolved. To monitor the fractionation, TLC of the eluates on silica gel in saturated chambers (mobile phase not specified) to a distance of 4 cm. Qualitative identification under UV 254 nm and by exposure to iodine vapor. The hRf values of the standard solutions of phenol, cresol and guaiacolin dichlorlomethane were 66, 68 and 78, respectively. Eluate fractions were further analyzed by GC–FID and GC–MS.

Biochem. Biophys. Res. Commun. 478, 1403-1408 (2016). Centrifugal TLC of xanthorrhizol in the rhizomes of Curcuma xanthorrhizza on silica gel with hexane mixed with increasing ratios of chloroform. Concentric spherical bands of the separated compounds were monitored under UV 254 nm and 366 nm on the rotor. When a single band came to the edge of the rotor, the eluate was collected.

Microbiol. Res. 194, 1-9 (2017). TLC of lipopeptide biosurfactant produced by the marine Aneurinibacillus aneurinilyticus SBP-11 on silica gel with 96 % ethanol – water 7:3 for peptides, chloroform – acetic acid – water 6:3:1 for sugars and chloroform – methanol – water 65:25:4 for lipids. Detection by spraying with ninhydrin reagent for peptides, anthrone reagent for sugars and rhodamine B reagent for lipids. The hRF values for peptides and lipids were 73 and 45, respectively.

and Cassia angustifolia Vahl. J. Planar Chromatogr. 30, 238-244 (2017). HPTLC fingerprint of sennoside A and B in Cassia senna L. and Cassia angustifolia on silica gel with 1-propanol ‒ ethyl acetate ‒ water 4:4:3. Detection by heating at 110 °C for 10 min, followed by spraying with 50 g/L potassium hydroxide in ethanol 50 % and heating again at 110 °C for 10 min. Qualitative determination at UV 366 nm.

J. Chromatogr. Sci. 54 (4), 492-499 (2016). Development of a sensitive and selective analytical method for simultaneous determination of aminoacridine hydrochloride (1) and lidocaine hydrochloride (2) in bulk powder and pharmaceutical formulation by TLC on silica gel with ethyl acetate – methanol – acetic acid 13:6:1. Quantitative determination by fluorescence measurement at 366 nm for (1) and by absorbance measurement at 220 nm for (2). The calibration curve was in the range of 25–250 ng/zone for (1) and 0.99–9.90 µg/zone for (2).

J. Chromatogr. Sci. 55 (5), 564-570 (2017). Determination of the chromatographic retention data for 13 new 5-arylidene-2,4-thiazolidinediones on cyano phase and RP-18 with 6 aqueous binary mobile phases modified with acetonitrile, methanol, ethanol, propanol, acetone and dioxane. Presentation of 3 attempts to find suitable quantitative structure–retention relationship (QSRR) models that quantify retention as a function of molecular descriptors. It was found that models built for RP-18 show generally better multiple R but are also mostly monoparametric with logP as the dominant descriptor. More informative from the standpoint of molecular interactions are QSRR models for cyano phase, and the quality of those models depends on the mobile phase modifier (the best was acetone and the worst propanol). It is suggested to consider extrapolated retention on cyano phase as alternative to standard RP-18 for further assessment of plasma protein binding, since all QSRR models use extrapolated retention as a property which is indirectly connected with plasma protein binding.

J. Planar Chromatogr. 30, 392-400 (2017). HPTLC of 13 Achillea species on silica gel with toluene – ethyl acetate – formic acid 50:49:1. Detection by spraying with anisaldehyde reagent, followed by heating at 126 °C for 10 min. TLC chromatograms were photographed and processed with ImageJ program. Also TLC on cyano phase with 2-propanol – n-heptane 1:1 and ethanol – water 3:2 for dichloromethane extracts and methanol – water 2:3 for methanolic extracts. Detection by spraying with 1 % solution of diphenylborinic acid 2-aminoethylester in methanol, followed by drying and spraying with 5 % solution of polyethylene glycol in methanol. Detection under UV 366 nm. Principal component analysis, similarity and distance measures were used to confirm the similarity between the studied samples.