Drug Standards of China 9 (2), 144-146 (2008). TLC of nisoldipine silica gel with chloroform - acetone - triethylamine - water 90:5:1. Detection under UV 254 nm. Semiquantification of impurities by comparison of spots. The method was successfully used for the quality control of real life samples.

J. Liq. Chromatogr. Relat. Technol. 31, 3020-3031 (2008). TLC of twelf thioazines on silica gel with chloroform - ethanol 10:1 and chloroform, and on aluminum oxide with dichloromethane and benzene - chloroform 1:1 in a chamber saturated for 30 min. Detection under UV 254 and 366 nm. The retention parameters were measured and then calculated as separation factors deltaRf, RS, and alpha. The hRf values were correlated with the dipole moments of thioazines and the symmetry of diazinodithiins.

J. Planar Chromatogr. 23, 162-165 (2010). TLC of iron(III), copper(II), nickel(II), cobalt(II), cadmium(II), zinc(II), silver((I), lead(II), bismuth(III), mercury(II), titanium(IV), manganese(II), and chromium(VI) on silica gel with 0.02, 0.1, 0.2, and 1.0 M aqueous sodium dodecyl sulfate (SDS) (M1), 0.2 M SDS + 0.04 M tartaric acid (9:1, 1:1, and 1:9) (M2); 0.2 M SDS + 0.08 M tartaric acid (9:1, 1:1, and 1:9) (M3); 0.2 M SDS + 0.1M tartaric acid (9:1, 1:1, and 1:9) (M4); 0.2 M SDS + 0.08 M citric acid (1:1) (M5); 0.2 M SDS + 0.08 M formic acid(1:1) (M6); 0.2 M SDS + 0.08 M acetic acid (1:1) (M7); 0.2 M SDS + 0.08 M oxalic acid (1:1) (M8). Detection reagents used were: 0.5 % dithizone in carbon tetrachloride, 1.0 % aqueous potassium ferrocyanide, 1.0 % ethanolic dimethylglyoxim, 1:1 2.0 M aqueous sodium hydroxide in 30 % hydrogen peroxide, and a methanolic silver nitrate solution. Quantitative analysis of zinc(II) by spectrophotometry after extraction. The detection limits were 0.85, 0.05, and 1.5 µg respectively for lead(II), zinc(II), and cobalt(II). The in-situ detection of cations was more sensitive than detection in solution.

Anal. Biochem. 409 (2), 249-259 (2011). Presentation of a less time-consuming, more sensitive, and more precise method for the quantitative determination of nucleoside triphosphates (NTPs), 5-ribosyl-1-pyrophosphate (PRPP), and inorganic pyrophosphate (PPi) in cell extracts by TLC: Separation of an acid extract of L. lactis by charcoal filtration into a filtrate and an eluate, which then was separated by TLC either in the Cashel solvent (0.85 M potassium phosphate, pH 3.4) or in the AFC solvent (3 M ammonium formate [pH 2.4] and 0.7 M ammonium chloride). Two-dimensional separation of 18 µL of the eluate sample using the AFC solvent in the first dimension and using 0.75 M LiCl in 7.5 % lithium borate (pH 6.8, borate solvent) in the second dimension.

International Journal of ChemTech Research 2(2), 807-812 (2010). Chloroform and ethyl acetate extracts of leaves and bark of Ficus mollis (Moraceae) were subjected to TLC fingerprint profiling on silica gel with toluene - ethyl acetate - formic acid 16:2:1. Evaluation under UV 254 nm. Derivatization with vanillin-sulfuric acid reagent, followed by heating at 105 °C until colorization. In the bark 7 well-defined zones were observed, whereas in leaves 10 zones were observed. Heavy metal and mineral analysis was performed by atomic absorption spectroscopy.

J. of Chromatogr. A 1218 (19), 2676-2683 (2011). A review on bioautography on TLC plates as an important means of detecting the biological activity of a sample. The technique requires only small amounts of sample, is ideal for the investigation of plant constituents which often occur as complex mixtures, and can be used for the target-directed isolation of these constituents. In contrast to HPLC, many samples can be run at the same time on TLC, and organic solvents, which cause inactivation of enzymes or death of living organisms, can be completely removed before biological detection. Many bioassays are compatible with TLC and antimicrobial, radical scavenging, antioxidant activities and enzyme inhibition tests can be applied.

Parasitol. Res. 107, 947-953 (2010). HPTLC of neutral lipids on silica gel (prewashed by development with dichloromethane - methanol 1:1 and dried for 30 min at 120 °C) with petroleum ether – diethyl ether – glacial acetic acid 80:20:1, detection by spraying with 5 % ethanolic phosphomolybdic acid reagent and heating at 115 °C for 10 min. HPTLC of polar lipids (phosphatidylcholine, phosphatidylethanolamine, sphingomyelin) with chloroform – methanol – deionized water 65:25:4, detection by spraying with 10 % cupric sulfate in 8 % phosphoric acid and heating at 140 °C for 30 min.

Acta Chromatographica 21(2), 203-215 (2009). HPTLC of commercial products containing Heterotheca inuloides, Citrus aurantium, Peumus boldus, Equisetum arvense, Eucalyptus globulus, Ginkgo biloba, Mentha piperita, Aloe vera, Salvia officinalis , and Cassia senna on silica gel with different mobile phases. The mobile phase for aloin, boldine, chlorogenic acid, rutin, kaempferol, caffeic acid, and quercetin was ethyl acetate – methanol – water 100:17:13; for menthol, cineole, menthone, alpha- and beta-thujone, geraniol, linalyl acetate and linalool it was toluene – ethyl acetate 93:7; for ginkolide B toluene – ethyl acetate – acetone – methanol 50:25:25:3; and for sennoside B ethyl acetate – formic acid – acetic acid – water 100:11:11:27. Detection with natural products reagent, anisaldehyde reagent or Liebermann-Burchard reagent. We found that in only 20 % of the 40 commercial products analysed the chromatographic characteristics of the respective plants matched those of the specific respective marker compounds. This highlights a problem arising from the lack of regulation of these products, and emphasizes the need to develop simple and reliable analytical methods like TLC methods that can be performed in any laboratory for the purpose of quality control of dietary supplements or commercial herbal products sold in Mexico.