Chinese J. of Veterinary Drugs 46 (5), 29-31 (2012). TCM is more and more important in veterinary medicine. It has been found that some medicines were illegally mixed with certain chemicals harmful to the animal body. Rapid screening of the additives illegally mixed in Astragalus polysaccharides injection by TLC on silica gel with 1) chloroform – methanol – acetone – ammonia 9:2:1:0.05 and 2) ethyl acetate – ethanol 1:1. Detection 1) under UV 254 nm; 2) by spraying with 5 % potassium iodobismuthate solution and evaluation in daylight; 3) by exposure to iodine vapor for a few minutes and evaluation under UV 254 nm. Identification of lincomycin, enrofloxacin, novalgin, 4-acetamino phenol, moroxydine hydrochloride, and ribavirin by comparison of the fingerprint with individual standards, with detection limits of 2.0, 0.5, 1.0, 0.5, 1.0, and 4.0 mg/mL, respectively. The method was applied to 25 batches of market samples and the results were compatible with those obtained by HPLC.

by thin-layer chromatography) (Chinese) Chinese J. Ethnomed. Ethnopharm.(3), 53-54 (2012) Melodinus fusiformis is a herbal TCM drug specially effective for curing rheumatism. TLC of the extractsand standard oleanolic acid of the crude drug on silica gel with n-hexane – ethyl acetate 7:3, detection by spraying with 5 % phosphomolybdate in n-propanol and heating using a air blower until the zones are visible.

J. Planar Chromatogr. 25, 89-90 (2012). TLC of sample solutions from a horizontal rotating tubular bioreactor on cellulose-coated TLC plates. Detection of metal ions by spraying with solutions of quercetine (0.1 g/100 mL 2-propanol) and dimethylglyoxime (1.0 g/100 mL ethanol), and exposure to ammonia vapor. Linearity was found to be in the range of 0.05-1.0 g/L.

Chinese J. Ethnopharm.(4), 51-52 (2012). Cassia fistula, as a crude drug of Dai nationality medicine, is the mucus from the dried pod of Cassia fistula L. It has special efficiency in relieving internal heat or fever, apocatastasis and relieving pains. Its pods and leaves are both used as the component crude drug in preparations. To identify different officinal positions of the drugs available in different places of origin, TLC of the extracts of the drug on silica gel with petroleum ether (60-90 ºC) – ethyl acetate – formic acid 15:10:1, detection by spraying with 5 % aluminum chloride in ethanol and heating at 105 °C, evaluation at UV 366 nm.

J. Planar Chromatogr. 25, 463-467 (2012). HPTLC of the enantiomers of the beta-blockers (bisoprolol, metoprolol, and propranolol) and a beta-2-agonist (salbutamol) on silica gel impregnated with Cu(II) complexes of L-threonine, L-serine, and L-tartaric acid, with different mobile phases. Limits of detection were within the range 0.19-0.26 µg for enantiomers of the selected compounds. L-Ser proved to be a very good ligand for exchange and successful enantio-resolution using common mobile phases.

J. Liq. Chromatogr. Relat. Technol. 36, 2497-2511 (2013). HPTLC evidence of rapid peptidization of L-proline and DL-proline on silica gel with 2-butanol - pyridine - ammonia (25 %) - water 39:34:10:26 and on silica gel plates impregnated with Mn(II) and Cu(II) acetate with dioxane - water 13:7+1 drop 2 % acetic acid. HPTLC enantioseparation of DL-proline was performed with 2-butanol - pyridine - glacial acetic acid - water 15:10:3:12. Detection by dipping into 0.5 % ninhydrin solution in 2-propanol, followed by heating for 2 min at 110 °C. Quantitative determination by absorbance measurement at 340 nm.

Chinese J. of Lishizhen Trad. Med. & Pharm. 23 (1), 176-178 (2012). Jiannao Bushen Pills are a traditional Chinese compound preparation effective for brain-strengthening, tonifying kidney, replenishing Qi to invigorate the spleen, relieve uneasiness of mind and body tranquilization. TLC on silica gel 1) for Angelica sinensis, with petroleum ether (60-90 ºC) – ethyl acetate 4:1, detection at UV 366 nm; 2) for Fructus Forsythiae, with petroleum ether (30-60 ºC) – methanol 20:1, detection by exposure to ammonia vapors for 15 min, then spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing under daylight; 3) for Cinnamomum cassia and cassia twig, with petroleum ether (60-90 ºC) – ethyl acetate17:3, detection by spraying with 0.4 % 2,4-dinitrophenylhydrazine in 12N hydrochloric acid and viewing under daylight; 4) for bighead atractylodes rhizome, with petroleum ether (60-90 ºC) – ethyl acetate 50:1, detection by spraying with 0.5 % p-dimethylaminobenzaldehyde in ethanol – sulfuric acid 5:1 and viewing under daylight; 5) for Radix Ginseng rubra, with chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing under daylight.

of Northern Pharmacy 9 (4), 4-5 (2012) Chongderi pills are a herbal traditional Mongolian medicine for treating diarrhea, invigorating the circulation of blood, stimulating the menstrual flow, and dissipating blood stasis, etc. For quality control, TLC on silica gel 1) for Radix aucklandiae, with benzene – methanol 27:1, detection by spraying with 1% vanillin in sulfuric acid – ethanol, detection under daylight; 2) for Rhizoma acori tatarinowii, with petroleum ether (60-90 º) – ethyl acetate 9:1, detection by exposure to iodine vapors, viewing under daylight; 3) for safflower carthamus, with ethyl acetate – formic acid – water – methanol 35:10:15:2, detection under daylight. Identification by comparison with the standards.