Journal of Pharmacognosy and Phytochemistry 4 (3), 21-24 (2015). Assessment of the quality of Mustadi Taila, an oil obtained from 8 plants (stems of Acacia chundra, Cedrus deodara, rhizome of Cyperus rotundus, seeds of Embelia ribes, roots of Glycyrrhiza glabra, Rubia cordifolia, Vitex negundo, Vetiveria zizanioides), and prescribed in Chakra Datta as a remedy for dental caries. TLC of the dichloromethane fraction of the aqueous oil extract with methanol – cyclohexane –dichloromethane 3:2:77. Evaluation at UV 254 and 366 nm revealed 10 prominent zones. Detection of alkaloids by spraying with Dragendorff’s reagent. Further properties of the oil (organoleptic as well as physico-chemical parameters, phytochemical screening, microbiological activity and heavy metals limits) were determined without TLC.

Innov. Food Sci. Emerg. Technol. 32, 127-135 (2015). HPTLC of prebiotic oligosaccharides produced in the orange juice on silica gel with acetonitrile - ethyl acetate - 1-propanol - water 17:4:10:18. Detection by spraying with 0.3 % 1-naphthyl ethylenediamine dihydrochloride in methanol with sulfuric acid 3 %, followed by heating at 120 °C for 10 min. The method was applied to determine the degree of polymerization. _x000D_

Phytochemistry. 119, 51-61 (2015). HPTLC of cochloxanthine (1) and dihydrocochloxanthine (2) in Cochlospermum species on silica gel with acetone - hexane 1:1. Detection by spraying with anisaldehyde reagent, followed by heating at 110 °C for 5-10 min. Identification under UV light at 254 nm and 366 nm. The hRF values for (1) and (2) were 53 and 49, respectively.

J. Liq. Chromatogr. Relat. Technol. 39, 242-248 (2016). Review of the application of multivariate TLC retention data, the theory of hierarchical cluster analysis, principal component analysis and parallel factor analysis. Different applications such as exploring retention, lipophilicity analysis, quantitative structure–activity relationship modeling, solvent selection and comparison with other techniques were described.

J. Planar Chromatogr. 29, 7-14 (2016). Simultaneous chromatography and electrophoresis of a dye mixture containing crystal violet, methylene blue, bromocresol green, and methyl red on silica gel with propanol – 50 mM glycine buffer 2:1 and a pressure of ~32 bar. Most separations occurred at 818 V. Nearest neighbor descriptors and relative spot capacities were used to determine separation quality with the proposed systems.

CBS 115, 5-7 (2015). HPTLC of xanthones in mangosteen fruit (Garcinia mangostana) on RP-18 with acetonitrile – water – formic acid 80:17:3 to a migration distance of 65 mm. Detection by spraying with NEU's reagent and PEG, evaluation under UV 366 nm. The hRf value of β-mangostin was 11, of 9-hydroxycalabaxanthone 14, of α-mangostin 29, and of γ-mangostin 43. Quantitative determination of the four xanthones as α-mangostin equivalents by absorbance measurement at 280 and 320 nm. Comparison with an HPLC-UV method on RP-18 showed only a small difference (1.5 %) of the quantified total content. HPTLC was found to be a good alternative to HPLC.

J. Planar Chromatogr. 29, 145-147 (2016). HPTLC of amino acids in seed cotyledons and seed coats from two types of legumes: black-eyed beans (Vigna unguiculata) and red kidney beans (Phaseolus vulgaris) on silica gel with n-butanol – glacial acetic acid – water 12:3:5. Detection by spraying with ninhydrin reagent 0.2 g/100 mL in ethanol, followed by heating at 80-100 °C for 5 min. The hRF values of essential and nonessential amino acids were determined and ranged between 13 and 62.

J. Chromatogr. Sci. 52 (9), 1089-1094 (2014). HPTLC of the anticancer compound nimbolide in different parts of Azadirachta indica and its dosage form on silica gel with n-hexane – ethyl acetate – acetic acid 30:20:1 (migration distance 68 mm, chamber saturation time 2 min), detection by spraying with 5 % sulfuric acid in methanol, quantification after absorption measurement at 515 nm. Validation by investigation of the (A) hRf value of nimbolide (43), (B) linearity range (200–1400 ng/zone, r2=0.99968), (C) LOD (70 ng/zone) and LOQ (200 ng/zone), (D) recovery (97.5 %, n=3), and (E) specificity (comparison of hRf value and UV/vis absorption spectrum with the standard).