Planta med. 68, 770-775 (2002). Preparative and analytical TLC of chelirubine, sanguinarine, macarpine, and chelerythrine on silica gel and aluminium oxide with chloroform - methanol 49:1. Detection under UV light.

Planta med. 68, 896-900 (2002). TLC of cichoric acid, echinacoside, maackianin, and umbelliferone on silica gel with ethyl acetate - AcOH - acetic acid - water 100:11:11:27 and formic acid - ethyl formate - toluene 3:10:10. Detection after spraying with 1 % 2-aminophenyl diphenyl borate in methanol and 5 % PEG 400 in methanol under UV light at 366 nm.

J. Chinese Trad. Patent Med. (Zhongchengyao) 25 (8), 627-629 (2004). TLC on silica gel 2-fold with petroleum ether - ethyl acetate 9:1. Detection 1) under UV 254 nm. Identification by comparison with the standards. Quantitation by densitometry at 300 nm. Validation of the procedure by investigation of its precision, specificity, repeatability, reproducibility, recovery, etc.

CBS 88, 14-15 (2002). HPTLC of mushroom extracts on silica gel with dichloromethane - ethyl acetate - methanol 3:1:1 and HPTLC of hydroquinone, rutin, resorcinol, and ascorbic acid on silica gel with toluene - methanol - acetic acid 45:8:4 over 80 mm with chamber saturation. Determination of bioactivity with DPPH-biotest by spraying with 5 mg (2,2-di-(4-tert-octylphenol)-1-picrylhydrazyl in 10 mL acetone.

Chinese Trad. Patent Med. (Zhongchengyao) 26 (1), 74-76 (2004). TLC on silica gel with 1) petroleum ether - ethyl acetate - formic acid 30:10:1, 2) petroleum ether - ethyl acetate 25:1, 3) chloroform - methanol - water 13:7:2. Detection 1) under 365 nm, 2) by spraying with 1 % vanillin - H2SO4 solution, 3) by spraying with 10 %H2SO4 in ethanol. Identification by fingerprint techniques, combined with microscopy of the microcosmic configuration and shape of the medicine, and with visualization by color reaction of anthracene and quinine with 1 % NaOH solution.

J. Planar Chromatogr. 17, 84-88 (2004). OPLC of trans-resveratrol on silica gel with chloroform - methanol 10:1 after preconditioning of the plates for 3 h at 120 °C. Detection by immersion of the dried plates in a bacterial suspension (Pseudomonas savastanoi pv. phaseolicola race 6) for 20 s and MTT staining (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide; 80 mg MTT and 100 mg Triton X-100 in 100 mL water) either after a short draining period or after overnight incubation; after staining the time for evaluation varied (e. g. from 1 h to 6 - 9 days or more). First results of the investigation of a complex bioautographic system with special emphasis on the direct effect of the antibiotic trans-resveratrol (fitoalexin) and the function of formaldehyde in relation to antibiotics.

J. Liq. Chrom. Rel. Technol. 27, 2113-2119 (2004). TLC of corrinoid compounds, authentic B12 and cyanocobamides (benzimidazolyl-, 5-hydroxy-benzimidazolyl- and 7-adenylcyanocobamides) on silica gel with 2-propanol - 28% ammonia - water 7:1:2 in the dark at 25 °C. Detection under daylight as pink-colored spots.

Indian Drugs 41 (3), 160-164 (2004). Combinations of ofloxacin and tinidazole (1:3) are available as tablet dosage forms. Stability indicating HPTLC of ofloxacin and tinidazole on silica gel with dioxan - ethyl acetate - toluene - acetic acid - water 5.5:5:3.2:2:2 . Quantification by absorbance measurement at 307 nm. The Rf value of ofloxacin and tinidazole was found to be 0.12 and 0.85 respectively. Recovery was 99.85 % and 99.45 % for ofloxacin and tinidazole respectively. The proposed method was quantitatively evaluated in terms of calibration of concentration range, stability, and accuracy. It can be adopted for routine analysis of the formulation.