J. Sep. Sci. 46, 2200805 (2023). HPTLC of paraconic acids in Cetraria islandica on silica gel with cyclohexane - ter-butyl-methyl-ether - formic acid 16:6:1. Detection by exposure to iodine vapor and spraying with a starch solution. Further analysis by LC-MS.

J. Ethnopharmacol. 317, 116732 (2023). HPTLC fingerprinting of stem bark of Berberis aristata on silica gel with n-butanol - glacial acetic acid - water 13:3:4. Detection under UV light at 366 nm. The method revealed a major zone at hRf value of 60.

Phytochem. Anal. doi:10.1002/pca.3252 (2023). HPTLC of tripterpenes in the roots of Cecropia angustifolia on silica gel with toluene - chloroform - ethanol 4:4:1. Detection by spraying with vanillin–sulfuric acid reagent. Further analysis by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry.

Phytochem. Anal. doi:10.1002/pca.3265 (2023). HPTLC of antidiabetic compounds in the leaves of Annona cherimola on silica gel with chloroform - propanol - ethyl acetate 21:2:2. Detection of α-amylase inhibitors by spraying with 4 mL of an enzyme solution (6 U/mL) in acetate buffer (0.5 M, pH 6.9), followed by incubation at 37 °C for 10 min, dipping into a 1 % starch solution for 3 s and incubation at 37 °C for 15 min. Inhibitory zones were detected by dipping into a Gram's iodine solution. Detection of α-glucosidase by spraying with 4 mL enzymatic solution (5 U/mL α-glucosidase in phosphate buffer), followed by incubation at 37 °C for 10 min. The enzyme cleaved the substrate producing α-naphthol, which was detected by spraying with 4 mL of an aqueous solution of Fast Blue B salt. Further analysis of active zones by UHPLC-DAD coupled with detector electrospray ionisation tandem mass spectrometry (ESI-MS/MS).

Food Res. Int. 165, 112472 (2023). HPTLC of galactolipids (monogalactosyldiacylglycerol and digalactosyldiacylglycerol) in chloroplast-rich pellet from spinach leaves on silica gel with chloroform - methanol - water 19:4:5. Detection by dipping into a thymol solution (1 g of thymol in 190 mL of ethanol following with a slow addition of 10 ml of sulphuric acid), followed by heating at 110 °C for 10 min. Image visualization under white light and under UV light at 254 and 366 nm.

Food Chem. 416, 135822 (2023). A chip consisting of two parts: a pesticide residue reaction and a separation area cut from a TLC plate was used for the analysis of pesticides dichlorvos, paraoxon and parathion in spiked cabbage, cucumber and spinach with 40 % dd water - acetonitrile solution. Once the pesticide was absorbed by the pesticide enrichment zone, the TLC plate was removed and allowed to dry in the air for 1 min, followed by adding the esterase enzyme solution (prepared from crushed malted barley) and incubation at 37 °C for 3 min. Detection by overlapping with a substrate cromogenic area impregnated with dichloroindophenol acetate. A scanner and digital image-processing was performed to quantify adsorbed substances. LOD was 2 ng/g for dichlorvos, 6 ng/g for paraoxon, and 3 ng/g for parathion.

Food Chem. 428, 136825 (2023). Review of phytochemical properties of Passiflora and methods for the identification of chemical compounds in different parts of the plant. The papers described TLC and HPTLC methods for the analysis of components such as p-hydroxibenzoic acid, syringic acid, apigenin.

Food Chem. 421, doi.org/10.1016/j.foodchem.2023.136217 (2023). HPTLC of scopoletin in cassava landraces on silica gel with ethyl acetate - dichloromethane - acetic acid - formic acid - water 23:6:2:2:2. Detection under UV light at 366 nm.