J. Planar Chromatogr. 24, 357-359 (2011). TLC of benzo[a]pyrene on RP-18, RP-8, and RP-2 with acetone - ethyl acetate 7:3 in an unsaturated twin-trough chamber. Detection by dipping for 1 s into a solution of 250 mg bis(2,4-dinitrophenyl)oxalate in 40 mL of n-butyl acetate and 5 mL of hydrogen peroxide (upper phase). For videodensitometric evaluation the sheets were measured for 2 min and monitored over a period of 2 h. The reaction energy is transferred to the fluorescent compound (benzo[a]pyrene) which emits light while relaxing from its excited state. The advantage of this chemiluminescence reaction performed on a TLC plate is the improvement of the detection limit by extending the measuring time. The detection limit is 50 pg benzo[a]pyrene which is identical to that achievable in chemiluminescence HPLC. The calibration curve for benzo[a]pyrene was linear in the range of 50-8000 pg. The new procedure overcomes the problem of uneven illumination with CCD cameras.

J. Planar Chromatogr. 24, 545-546 (2011). TLC of carbosulfan and other commonly available carbamate pesticides, organochlorine pesticides, organophosphorus pesticides, synthetic pyrethoids and extracts from biological tissues on silica gel with toluene - acetone 9:1 with chamber saturation for 30 min. Detection by spraying with 10 % sodium hydroxide solution followed by 4-aminoantipyrine reagent and then with potassium hexacyanoferrate(III) reagent. The red-colored zone for carbosulfan was observed immediately. Carbaryl, propoxur, and carbofuran form a red-colored zone at the hRf values of 52, 45, and 48, respectively, whereas the hRf value of carbosulfan was 94.

Planta Med. 76, 133-136 (2010). TLC of mitraphylline on silica gel with (1) dichloromethane - acetone 5:4, (2) diethyl ether - ethyl acetate 1:1, and (3) dichloromethane - ethanol 19:1. The hRf value in system (1) was 83, in (2) 73, and in (3) 68. Detection by spraying with sulfuric acid - acetic acid - water 1:20:4 followed by heating at 120 °C and by Dragendorff’s reagent.

J. of China Pharm. 20 (9), 16-17 (2011). TLC of the extracts of Biyan syrup 1) for Herba Ephedrae, on silica gel with chloroform - methanol - ammonia 40:10:1, detection by spraying with ninhydrin reagent and heating at 105 ºC; 2) for Flos Magnoliae, on silica gel with chloroform - diethyl ether 5:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC; 3) for Radix Astragali, on silica gel with ethyl acetate - butanone - formic acid - water 5:3:1:1, detection by spraying with 5 % iron(III) chloride in ethanol; 4) for Angelica dahurica (Fisch.) Benth. et Hook, on silica gel with toluene - petroleum ether (60-90 ºC) - ethyl acetate 6:3:1, detection under UV 366 nm.

J. Planar Chromatogr. 24, 201-205 (2011). TLC of sixteen porphyrins on RP-18 with methanol or methanol - chloroform 4:1 with chamber saturation for 30 min at room temperature. Detection by visual identification. Determination of the experimental log P using RP TLC together with the use of log PRekker, which was calculated on the basis of fragmentation constants available in the literature.

Chinese J. of Ethnomed. & Ethnopharm. 23, 61-62 (2010). Preparation of the samples by extracting Saururus chinensis (Lour.) Baill with methanol - 25 % hydrochloric acid 4:1 and sonication for 1 h (these were the best conditions of five different solvent compositions and different sonication times investigated). TLC of the obtained extracts on silica gel with 1) petroleum ether (60-90 ºC) - acetone 5:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC; 2) toluene - ethyl acetate - formic acid 5:2:1, detection by spraying with 1 % aluminium chloride in ethanol and evaluation under UV 366 nm; 3) hexane - ethyl acetate - formic acid 70:50:8, detection by spraying with 1 % aluminium chloride in ethanol and heating at 105 ºC, detection under daylight and UV 366 nm; 4) toluene - ethyl acetate - formic acid 5:4:1 saturated with hydrochloric acid, detection under daylight and UV 366 nm. System 4) provided the best separation and was most practical. Identification of quercetin by fingerprint comparison with the standard.

Phytochem. Anal. 24, 47-52 (2013) TLC of 22 acylated phenolic compounds on silica gel with acetonitrile - water - chloroform - formic acid 12:3:2:1, followed by dipping into 0.2 % methanolic 2,2-diphenyl-1-picrylhdrazyl solution (DPPH radical reagent) for 5 s and kept in the dark for 30 min. Free radical scavenging activity of the acylated phenolic compounds was assessed by coupling with the image processing software ImageJ .

J. Planar Chromatogr. 25, 361-362 (2012). HPTLC of pigments in wheatgrass on sucrose-impregnated silica gel prepared by dipping the plates into a sucrose solution (400 g granulated sucrose/1 L distilled water), followed by heating at 110 °C for 20 min. Development with n-hexane - acetone 37:13. Detection by absorbance measurement between 400 and 800 nm. Sucrose impregnation prevents the degradation of the pigments during the analysis.