Microbiol. Res. 192, 114-121 (2016). TLC of vincamine indole alkaloids from Nerium indicum on silica gel with mixtures of petroleum ether – ethyl acetate 1:8, 1:4, 1:2 and 1:1. The hRF value for vincamine indole alkaloid was 56.

J. Liq. Chromatogr. Relat. Technol. 40, 239-246 (2017). Review of TLC methods for the analysis of polyphenols, dyes, carboxylic acids, biogenic amines, and vitamin C in quality assessment and authentication of non-fermented or fermented beverages derived from fruits.

Part II: Remijia ferruginea. Rev. Bras. Farmacogn. 27, 153-157 (2017). HPTLC fingerprint of Remijia ferruginea on silica gel with ethyl acetate – toluene – formic acid – water 12:4:4:3. Detection by spraying with natural product reagent, followed by PEG reagent. The hRF values for rutin, chlorogenic acid and cinchonine were 50, 60 and 65, respectively.

J. Chromatogr. A 1473, 133-142 (2016). Development of a simple, accurate and precise method for the analysis of proton pump inhibitors and their co-formulated drugs, available as binary combination, by HPTLC on silica gel with toluene – iso-propranol – acetone – ammonia 50:23:25:2. Identification of 14 analytes (except for rabeprazole and lansoprazole which could not be separated) based on hRf and recorded peak spectra. Qantitative determination by densitometry at 290 nm. The linear response for the selected drugs was good with correlation coefficients ≥0.9993. The LOD and LOQ was between 7-160 ng/band and 21-478 ng/band, respectively, for all the analytes. Assessment of the method performance by analyzing different laboratory simulated mixtures and some marketed formulations of the selected drugs, and successful application of the method to investigate potential counterfeit of proton pump inhibitors through a series of simulated formulations with good accuracy and precision.

J. Planar Chromatogr. 30, 401-404 (2017). HPTLC of 35 model compounds on RP-8 with 61 chromatographic systems to determine the solvent strength of 20 solvents on_x000D_ RP-8 phase and to compare the values with a previous study on silica gel. The study showed that the RP-8 phase can behave as normal-phase adsorbent.

J. Planar Chromatogr. 31, 13-22 (2018). HPTLC of phenolic acids (chlorogenic acid, rosmarinic acid, protocatechuic acid, gallic acid, syringic acid, ellagic acid, trans-cinnamic acid, o-coumaric acid, m-coumaric acid, p-coumaric acid, caffeic acid, ferulic acid, sinapic acid) and some flavonoids (flavone, apigenin, luteolin, chrysin, quercetin, myricetin, kaempferide, kaempferol, hesperetin, naringenin, pinocembrin) on silica gel with n-hexane ‒ ethyl acetate ‒ formic acid 6:4:1. Detection at UV 280 nm and 330 nm. The method was combined with mass spectrometry to test the applicability on various complex matrix samples, such as propolis, roasted coffee, and rose hip crude extracts.

platychlaena fruit essential oils. J. Planar Chromatogr. 31, 61-71 (2018). Overpressured layer chromatography (OPLC) of two Prangos platychlaena ssp. platychlaena fruit essential oils on silica gel with toluene – isooctane 4:1, external pressure 50 bar, flow rate 100 mL/min. Bioautography by spraying with a spore suspension (105 spores/mL) of Colletotrichum species without chemical derivatization, followed by incubation for 4 days at 26 °C with a 12 h photoperiod. Detection by spraying with vanillin–sulfuric acid reagent (0.1 g vanillin in 100 mL ethanol and 2 mL sulfuric acid), followed by heating at 110 °C for 3 min.

J. Chromatogr. A 1530, 211-218 (2017). Development of a novel and cost-effective TLC method on cellulose (instead of silica gel) for authentication of selected fruit-based alimentary products. As authenticity markers the anthocyanins cyanin chloride, keracyanin chloride, pelargonidin chloride and delphinidin chloride were used. With TLC, the LOD and LOQ for cyanin were of 25 and 75 ng/zone, for keracyanin 55 and 166 ng/zone, for pelargonidin 47 and 140 ng/zone, and for delphinidin 171 and 513 ng/zone. With HPTLC the LOD and LOQ for cyanidin were 107 and 321 ng/zone, for keracyanin 189 and 566 ng/zone, and for pelargonidin 161 and 484 ng/zone (delphinidin was not detectable). Consequently, quantification of anthocyanes in the alimentary products by TLC allowed identification of more target compounds and in a higher number of alimentary products than by HPTLC. (Note that original HPTLC method in J Chromatogr A 1299 (2013) 105-118 was reported to be more sensitive (mainly 3-50 ng/zone) and with higher correlation coefficients of calibration curves (0.9993-0.9999) for 11 anthocyanins/-cyanidins than the HPTLC method that was reproduced in this paper.)