J. Chinese Trad. Patent Med. (Zhongchengyao) 25 (10), 785-787 (2004). Study of the optimum inclusion process conditions for volatile oil by using orthogonal design and determining the utilization ratio of volatile oil from Fructus Amomi Rotundus by TLC on silica gel with benzene - ethyl acetate 19:1 and detection by spraying with vanillin - H2SO4 solution. Establishment of the optimum conditions as volatile oil: beta-cyclodextrin 1:8 at 50 ºC for two hours, giving the utilization ratio of volatile oil of 70.7%.

J. Chinese Trad. Patent Med. (Zhongchengyao) 25 (9), 719-720 (2004). TLC on silica gel with 1) chloroform - methanol - water 13:7:2, 2) chloroform - acetone - formic acid 10:5:2, 3) petroleum ether - ethyl formate - formic acid 15:5:1. Detection 1) by spraying with 10 % H2SO4 in ethanol and heating and under UV 365 nm, 2) by ammonia vapor and under UV 365 nm. Identification by fingerprint techniques. Quantitation of danshensu by HPLC. Discussion of use of the procedures for the quality control of the medicine.

J. Planar Chromatogr. 17, 95-101 (2004). TLC of 15 flavonoids (flavanone, naringenin, flavone, 3-, 6-, 6’-, 7-hydroxyflavone, 3,6-, 3,7-dihydroxyflavone, morin, chrysin, quercetin, galangin, apigenin, kaempferol) and 4 phenolic acids (o-, p-coumaric acid, caffeic acid, ferulic acid) on silica gel after prewashing with methanol with 11 mobile phases. Chloroform - methanol - 98-100 % formic acid 441:30:235 and n-hexane - ethyl acetate - glacial acetic acid 31:14:5 were the most appropriate eluents. Detection under UV light at 254 and 366 nm or after spraying with 1 % methanolic diphenylboryloxyethylamine and 5 % ethanolic poly(ethylene glycol) 4000, and under UV light at 366 nm after spraying with 1 % ethanolic aluminum chloride solution.

J. Planar Chromatogr. 17, 54-57 (2004). TLC of food pigments (patent blue V, quinoline yellow, brilliant blue FCF, tartrazine, azorubine, ponceau 4 R, curcumine, indigo carmine, cochineal, methyl violet, mixed carotenes, plain caramel, erythrosine B, orange yellow S) and artificial sweeteners (aspartame, acesulfame, sodium cyclamine) and benzoic acid on silica gel. Pigments were developed with isopropanol - 12.5 % ammonia 5:1; sweeteners and benzoic acid were developed with ethanol - isopropanol - 12.5 % ammonia 10:40:1. Derivatization with a solution of 0.04 g bromocresol green in 100 mL ethanol containing 0.1 M sodium hydroxide.

Chinese J. Trad. Patent Med. (Zhongchengyao) 26 (3), 192-195 (2004). TLC of Qufeng medicinal wine on silica gel with 1) n-hexane - ethyl acetate 9:1; 2) ethyl acetate - methanol - water 100:17:13; 3) toluene - ethyl acetate - formic acid - water 20:10:1:1; 4) chloroform - methanol - water 40:10:1; 5) n-hexane - ethyl acetate - chloroform - glacial acetic acid 50:15:5:1. Detection 1) under UV 365 nm; 2) by spraying with 1 % AlCl3 in methanol and under UV 365 nm; 3) by spraying with 5 % H2SO4 in ethanol and heating at 105 ºC; 4) by exposing to ammonia vapor. Identification by fingerprint technique. Quantification of emodin by densitometry at 460 nm. Validation of the method by investigation of its linearity range (0.133-1.330 µg/spot, r=0.9999), precision (RSD=1.44 %, n=6), recovery (99.24 %, RSD=0.74 %, n=12).

Chinese J. Trad. Patent Med. (Zhongchengyao) 26 (5), 374-376 (2004). TLC of Qingdai powder extract on silica gel with 1) benzene - chloroform - acetone 5:4:1; 2) petroleum ether (60-90 ºC) - benzene - ethyl acetate 9:2:1; 3) benzene - ethyl acetate - isopropanol - methanol - water 60:30:10:50:15:3; 4) petroleum ether (30-60 ºC) - benzene - ethyl acetate - glacial acetic acid 20:40:14:1. Detection 1) under day light; 2) by spraying with 5 % vanillin-H2SO4 solution and heating at 105 ºC ; 3) under UV 365 nm; 4) under UV 254 nm. Identification by fingerprint techniques. Quantification of indigo by HPLC with method validation.

J. Planar Chromatogr. 17, 459-463 (2004). TLC of standard solutions of nine flavonoids and six phenolic acids (cinnamic, o-coumaric, m-coumaric, p-coumaric, caffeic, ferulic acid, galangin, quercetin, pinocembrin, naringenin, apigenin, chrysin, kaempferol, morin, acacetin) on silica gel in pre-saturated developing chambers with 1) n-hexane - ethyl acetate - glacial acetic acid 31:14:5, or 2) chloroform - methanol - formic acid 88:7:5. After drying, bands were visualized under short- and long-wavelenghth UV light. Detection by spraying with 1 % aluminium trichloride solution and evaluation under long-wavelength UV light. Standards were chromatographed again with a propolis extract. First, plates were developed with mobile phase 1 (or 2), the eluent was evaporated, standard solutions were applied again, and the plate was rotated through 90 ° and chromatographed again with mobile phase 2 (or 1). The presence (or absence) of all standards was determined according to their Rf values and fluorescence colors. Quantitative determination by absorbance measurement at 254 and 366 nm.

J. Planar Chromatogr. 17, 342-349 (2004). TLC of 29 1,2,3,4-tetraoxanes on silica gel, cyano phase, and RP-18. The binary mobile phases ethyl acetate - petroleum ether and ethyl acetate - toluene were used under normal-phase conditions, and water - organic modifier (methanol, acetone, dioxane) under reversed-phase conditions. Chromatography was performed using a HPTLC horizontal developing chamber equilibrated for 15 min with the vapor of the mobile phase. Detection by spraying with 50 % sulfuric acid and heating until the spots became visible.