LC-GC Europe 18, 482-488 (2005). Contact Bioautography: Antimicrobials diffusion from a TLC plate to an inoculated agar plate.The chromatogram is placed face down onto the inoculated agar layer and left for some minutes or hours for diffusion. After removing the plate the inhibition zones are observed on the agar surface in the places where the spots of antimicrobials are stuck to the agar. The method resembles a disk assay. Immersion Bioautography: The chromatogram is covered with a molten, seeded agar medium. After solidification, incubation and staining (usually with tetrazolium dye) the inhibition or growth bands are visualized. Direct Bioautography: A developed plate is dipped in the suspension of microorganisms growing in a suitable broth or this suspension is sprayed onto the plate. The plate is incubated and microorganisms grow directly on it. It can be performed with Photobacterium phosphoreum (Vibrio fischeri) suspension. Bioautography systems and coupling possibilities are presented.

J. Planar Chromatogr. 21, 387-390 (2008). TLC of prostaglandin PGE2 and PGF2a on silica gel and RP-18 in a saturated chamber with methanol - dichloromethane 1:9 and 100 % acetonitrile, respectively. Detection by spraying with phosphomolybdic acid (10 % in methanol) and heating at temperatures ranging from 80 to 100 °C (for silica gel) and below 80 °C (for RP-18). Densitometric evaluation; chromatographic spot quantification, however, was performed manually using the peak height above baseline method.

J. Chinese Trad. Patent Med. 30 (12), 1781-1785 (2008). TLC of Shenyi granule extracts on silica gel with 1) dichloromethane - ethyl acetate - petroleum ether (60-90 ºC) - methanol 20:14:6:1; 2) ethyl acetate - formic acid - glacial acetic acid - water 30:3:4:3; 3) toluene - ethyl acetate - formic acid 8:6:1; 4) chloroform - methanol - water 26:10:3. Detection 1) by spraying with 10 % sulfuric acid in ethanol and heating; 2) by spraying with iron(III) chloride solution; 3) by spraying with vanillin reagent. Identification by comparison with the standards glycyrrhizic acid and glycyrrhetinic acid.

J. Planar Chromatogr. 22, 157-161 (2009). Safed musli is the common name for Chlorophytum borivilianum, whereas shatavari is Asparagus racemosus, both of the liliaceae family. HPTLC of saponins and sapogenins on silica gel with chloroform - methanol - water 32:25:5 for saponins and with petroleum ether - ethyl actate 4:1 for sapogenins. Detection of saponins by spraying with anisaldehyde reagent and with Ehrlich’s reagent, followed by densitometric analysis at 620 and 510 nm. Detection of sapogenins by spraying with Liebermann Burchard reagent, followed by densitometric analysis at 580 nm.

J. Planar Chromatogr. 21, 251-258 (2008). TLC of tiaprofenic acid, ketoprofen, naproxen, dexibuprofen, flurbiprofen, alminoprofen, and ibuprofen on silica gel with chloroform - acetone - toluene 12:5:2 and chloroform - ethyl acetate 1:1. Quantitative determination by absorbance measurement at 225 nm (for naproxen, dexibuprofen, and ibuprofen) and at 270 nm (for tiaprofenic acid, ketoprofen, flurbiprofen, and alminoprofen).

Anal. Chem. 81, 9274-9281 (2009). TLC of dye standards (FD&C Green No. 3, FD&C Red No. 3, eriochromcyanin R), drug standards (3,4-methylene-dioxy-N-methamphetamine, lysergic acid diethylamide, flunitrazepam), and rosemary essential oil on 1) RP-18 with 65 % acetone containing 1.5 % formic acid and 2) on silica gel with chloroform - methanol 9:1. Rosemary essential oil was also separated on silica gel with toluene - ethyl acetate 9:1. Detection under UV 254 nm and in daylight. The combination of laser-induced acoustic desorption and electrospray ionization mass spectrometry (LIAD/ESI/MS) can be used to rapidly characterize chemical compounds separated on a TLC plate.

Acta Chromatographica 20 (4), 637-642 (2008). TLC of thiamine hydrochloride from riboflavin, nicotinic acid, calcium D-pantothenate, pyridoxine hydrochloride, cyanocobalamin, and ascorbic acid on silica gel with dioxane - water 1:1. Detection under UV light. Examination of the effect of impurities (metal cations and inorganic anions) on the chromatography of thiamine hydrochloride. The detection limit for thiamine hydrochloride was 90 ng/spot and %RSD of thiamine hydrochloride was 14.9 % (n=5).

60th Indian Pharmaceutical Congress PA-224 (2008). HPTLC of aloe-emodin in extract of Rheum Emodi (prepared by supercritical fluid extraction) on silica gel with toluene - acetone - formic acid 80:20:1. Quantitative determination by absorbance measurement at 254 nm. Linearity was in the range of 100-400 ng/spot. Compared with other extraction techniques supercritical fluid extraction was more effcient and less time consuming.