Food Control. 70, 119-129 (2016). HPTLC of aflatoxins AFB1, AFB2, AFG1 and AFG2 in Brazil nut (Bertholletia excelsa) on silica gel with toluene – ethyl acetate – formic acid 5:4:1. Qualititative identification under UV light at 366 nm.

J. Planar Chromatogr. 30, 225-230 (2017). HPTLC of chlorpromazine, chlorprothixene, clozapine, quetiapine, perazine, perphenazine, prochlorperazine, promazine and trifluoperazine on silica gel (1) and amino phase (2) with tetrahydrofuran – petroleum ether ranging from 40 % to 100 % and methanol – petroleum ether ranging from 10 % to 50 % for (1) and a mixture of tetrahydrofuran – water ranging from 50 % to 100 % for (2). Detection by spraying with a mixture of concentrated sulfuric acid – methanol 1:4, followed by heating_x000D_
at 120 °C for 10 min. A statistical analysis was used for the development of the quantitative structure‒retention relationship (QSRR) model._x000D_

Food Chem. 239, 848-857 (2018). HPTLC of lipids in raw milk on silica gel with n-hexane – diethyl ether – acetic acid 85:15:2. Detection by spraying with phosphomolybdic acid in ethanol. Quantitative determination by absorbance measurement at 650 nm. The identified peaks corresponded to phospholipids, 1,3-diglycerols, 1,2-diglycerols, sterols, free fatty acids and triacylglycerols.

J. Chromatogr. Sci. 54 (4), 647-652 (2016). Presentation of a sensitive, accurate and selective method for the simultaneous determination of paracetamol (PAR), its toxic impurity 4-aminophenol (4-AP), pseudoephedrine HCl (PSH) and loratidine (LOR). HPTLC on silica gel with acetone – hexane – ammonia 40:50:1. Densitometric evaluation at 254 nm for PAR, 4-AP and LOR, and at 208 nm for PSH. The method was used for the determination of PAR, PSH and LOR in commercial tablets and in plasma in the ranges of 0.5–6.0 µg/zone, 1.6–12.0 µg/zone and 0.4–2.0 µg/zone for PAR, PSH and LOR, respectively. Comparison of the results obtained by the proposed method with those obtained by HPLC method showed no significance difference between both methods regarding accuracy and precision.

J. Planar Chromatogr. 31, 36-47 (2018). Mineralogical and physicochemical characterization of the Nevşehir tuff and its application as a stationary phase for the separation of polar compounds such as amino acids or food dyes. Plates were coated with a slurry consisting of a mixture of adsorbent (Mirșid tuff or_x000D_ Nevşehir tuff), binder (polyvinyl alcohol or gypsum), and other admixtures. The impregnation of the Nevşehir tuff with sodium hydroxide and sodium chloride, respectively, allowed the separation of some hydrophilic dyes.

Phytochem. Anal. 28, 87-92 (2017). HPTLC fingerprint of the aerial parts of Justicia secunda on silica gel with chloroform – methanol – water 6:4:1. Detection by dipping into 200 mL enzyme solution (2000 U of alpha-D-glucosidase dissolved in 200 mL buffer solution prepared with 10 g sodium acetate in 250 mL double-distilled water, pH 7.5 with acetic acid), followed by incubation for 1 h in a humid desiccator and dipping into 200 mL of substrate solution (2 mg/mL 2-naphthyl-alpha-D-glucopyranoside in 50 % aqueous ethanol and 2.5 mg/mL Fast blue salt B in double-distilled water, mixed 1:1 before applying). Detection under UV 366 nm.

J. Planar Chromatogr. 31, 87-91 (2018). HPTLC-direct bioautography of the ethanolic leaf extract of greater burdock (Arctium lappa L.) on silica gel with chloroform – methanol – water 36:4:1. Direct bioautography by dipping into a bacterial suspension of Bacillus subtilis F1276 and Pseudomonas maculicola, followed by incubation for 2 h and dipping into an aqueous solution of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) vital dye. The main antibacterial compound was analyzed by HPLC-MS.

J. Chromatogr. A 1530, 185-191 (2017). Development of a new spray-on method for applying yeast cells to HPTLC plates, leading to a much higher sensitivity of the planar yeast estrogen screen (p-YES), which can serve as a highly valuable and sensitive screening tool for the detection of estrogenic compounds in various sample matrices such as water and wastewater, personal care products and foodstuff. HPTLC of sample constituents and direct detection of estrogenic compounds by spraying with yeast cells. This resulted in much sharper signals compared to those in previous publications. Satisfying results were achieved using cultures with cell densities of 1000 FAU with reduced signal broadening, thus lower LOQ for estrogenic compounds, e.g. estrone 2 pg/zone, 17β-estradiol 0.5 pg/zone, 17α-ethinylestradiol 0.5 pg/zone and estriol 20 pg/zone. Demonstration of the possibility of the method to characterize profiles of estrogenic activity of wastewater samples with high quality and reproducibility by using native samples from wastewater or even surface water directly applied on HPTLC plates without the need for prior sample treatment.