J. Liq. Chromatogr. Relat. Technol. 38, 1392-1406 (2015). HPTLC of Cordyceps sinensis and Ganoderma lucidum on silica gel with ethyl acetate - dichloromethane - formic acid - glacial acetic acid - methanol 10:10:1:1:2. Qualitative identification at UV 254 nm and UV 366 nm. Hyphenation of electronspray ionization/mass spectrometry with HPTLC facilitated fast and convenient profiling of the metabolites in the extracts. For the chemometric analysis, raw and column data matrices were constructed using hRF datasets.

J. Braz. Chem. Soc. 26, 1617-1624 (2015). RP-TLC of newly synthesized thiosemicarbazides and their cyclic analogues 1,2,4-triazol-3-thiones on RP-18 with methanol - water mixtures 45-80 %. Qualitative determination under UV light at 254 nm. The log P values of 1,2,4-triazole-3-thione derivatives were determined.

PLoS ONE 8(3), e57908 (2013). Feline SD (Sandhoff disease) fibroblasts GM2 SV3 and human TSD (Tay-Sachs disease) glial cells were transfected to express hybrid subunits H1 and H2 of beta-hexosaminidase A (Hex) and grown for 18 h in medium containing 4.7 µg/mL NBD-GM2 (2-nitro1,3-benzoxadiazol fluorescent derivative of GM2 ganglioside, substrate of Hex) and 50 µM CBE (conduritol-B-epoxide). To check the transfection, HPTLC of glycosphingolipids, extracted according to Folch’s method into acidic and neutral glycolipids, on silica gel first with chloroform – acetone 1:1, followed by chloroform – methanol – 0.2% CaCl2 55:45:10; the separated gangliosides (NBD-GM2 and its breakdown NBD-products) were quantified by a fluor image analyzer (Storm Imager). Total ganglioside fractions obtained through ion-exchange chromatography from transfected SD lysates were incubated with NBD-GM2 and separated on HPTLC, with the same first mobile phase followed by chloroform – methanol – water 65:25:4; the reaction product NBD-GM3 appeared only when GM2 activator protein was added during the incubation.

J. Chromatogr. Sci. 53 (5), 816-823 (2015). HPTLC of ursolic acid, betulinic acid, stigmasterol and lupeol in Shankhpushpi botanicals on silica gel in a twin-trough glass chamber with petroleum ether - ethyl acetate - toluene 7:2:1, detection by spraying with anisaldehyde reagent. Densitometric evaluation at 580 nm. The hRF value of ursolic acid was 21, of betulinic acid 29, of stigmasterol 33 and of lupeol 50. Linearity was between 100-600 ng/zone for all substances. Results of the analysis of real life samples: 134.2 mg/g and 146.1 mg/g ursolic acid in Clitorea ternatea (CT) and Canscora decussata (CD); 110.6 mg/g betulinic acid in Evolvulus alsinoides (EA); 92.8 mg/g, 154.9 mg/g, 31.9 mg/g and 39.2 mg/g stigmasterol in EA, Convolvulus pluricaulis, CT and CD; 30.1 mg/g lupeol in CT.

J. Planar Chromatogr. 29, 287-298 (2016). HPTLC of 34 structurally diverse drugs on RP-18 with acetonitrile – pH 7.4 phosphate buffered saline 7:3. Detection under UV 254 nm. Molecular descriptors were investigated to assign the compounds to two clusters: central nervous system active (CNS+) or inactive (CNS-) describing the ability of the compounds to penetrate the brain.

J. Planar Chromatogr. 29, 318-322 (2016). HPTLC of trans-resveratrol, aromatic acids (benzoic, cinnamic, ferulic, salicylic and caffeic acid) and flavones (flavone, daidzein, chrysin, kaempferol, quercetin and morin) in coffee, tea and wine on LiChrospher® plates with ethyl acetate – cyclohexane – 1-butanol 9:9:2 for trans-resveratrol and ethyl acetate – methanol – water 77:13:10 for aromatic acids and flavones. Chemiluminescence was induced by dipping the plate into a bis(2,4,6-trichlorophenyl)oxalate (250 mg in 36 mL 1-butyl acetate followed by shaking with 0.4 mL hydrogen peroxyde 35 % for 20 min) solution for 1 s. Fluorescence evaluation under UV 366 nm.

CBS 115, 2-4 (2015). HPTLC of propolis tinctures and standards estrone, 17-estradiol, 17-ethinylestradiol, estriol, bisphenol A, 4-n-nonylphenol, and caffeic acid methyl ester on RP-18W with n-hexane – toluene – ethyl acetate 8:3:2 to a migration distance of 7 cm. For the HPTLC-pYES bioassay the plate was dipped into a yeast cell suspension and incubated for 3 h. Biodensitometric evaluation by fluorescence measurement at 365 nm with a cut-off filter of 400 nm. Elution of the bioactive zones with methanol – ammonium formate buffer (10 mM, pH 4, 49:1) into a ESI-MS. The LOD for 17-estradiol was 0.2 pg/zone and the LOQ 0.5 pg/zone. With this method estrogen effective substances can be detected with no or minimal sample preparation.

J. of Chromatogr. Sci. 52 (1), 5-11 (2014). Presentation of a method for the simultaneous determination of diacerein in the presence of rhein, the active metabolite and hydrolytic degradation product of diacerein, and emodin, the diacerein impurity, in bulk powder and different pharmaceutical formulations. TLC on silica gel with hexane – ethyl acetate – acetic acid 75:50:1, detection and quantification by densitometry at 230 nm. The hRf values were 12, 44 and 60 for diacerein, rhein and emodin, respectively. The linearity ranges were 0.5–10 µg/band for diacerein and rhein, and 0.5–7 µg/band for emodin. Comparison with a HPLC method showed no significant differences.