Chinese J. Ethnomed. Ethnopharm. 1, 55-66 (2011). TLC of the extracts of Baibanting 1) for Gardenia jasminoides Ellis, on silica gel with ethyl acetate - acetone – methanol – water 5:5:1:1, detection under daylight and by spraying with 10 % sulfuric acid in ethanol and heating at 110 ºC; 2) for Cuscuta chinensis Lam, on polyamide phase with methanol - glacial acetic acid - water 4:1:5, detection by spraying with 3 % alumnium chloride in ethanol, evaluation under UV 366 nm; 3) for Malaytea scurfpea fruit, on silica gel with n-hexane - ethyl acetate 4:1, detection by spraying with 10 % KOH in methanol and evaluation under UV 366 nm. Quantification of gardenoside by HPLC. The procedures proved to be simple, accurate, reproducible, robust and suitable for the quality control of the medicine.

J. Liq. Chromatogr. Relat. Technol. 32, 2893-2905 (2009). HPTLC of albiflorin, paeoniflorin, (beta)-catechin, benzoyloxypaeoniflorin, benzoylpaeoniflorin, beta-sitosterol in the roots of Radix paeoniae Rubra (1) and Radix Paeoniae Alba (2) on silica gel with chloroform - ethyl acetate - methanol - formic acid 30:5:10:1 for high polarity components and toluene - ethyl acetate - methanol - formic acid 20:4:2:1 for lipophilic components. Detection by spraying with vanillin - sulphuric acid - ethanol 1:5:95, followed by heating at 105 ºC for 10 min. Qualitative determination by densitometry at 366 nm. The HPTLC fingerprints allowed differentiation between the roots of (1) and (2).

Food Chemistry 132, 671-674 (2012). TLC of saponins on silica gel with n-butanol - water - acetic acid 12:2:1. Detection by dipping into a suspension of sheep erythrocytes for 20 s, then plates were taken out and held vertically for 30 s. White spots against a pink background appeared. The plate was immersed in phosphate-buffered saline for 30 s to remove excess blood on the plate surface and again held vertically for 30 min. The method is simple, specific, convenient and time saving for analysis of saponins by TLC for purification, chemoprofiling of plants, and nutraceutical applications.

J. Planar Chromatogr. 24, 348-351 (2011). Study of the lipophilicity and specific hydrophobic surface area of fourteen 1,3-benzoxazol-2(3H)-ones substituted in the benzene ring (fluoro-, chloro-, bromo-, dibromo-, amino-, and nitro-derivatives). TLC on RP-18 with methanol - water, methanol - aminoacetic acid buffer pH 2.7, and methanol - aminoacetic acid buffer pH 11.6. The concentration of methanol in the mobile phase ranged from 30-90 % in all cases. Detection under UV 254 nm. The linear correlation between the volume fraction of methanol and values over a limited range were established with good correlation coefficients (r > 0.98). The obtained results were compared with calculated partition coefficients.

J. of Chromatogr. A 1251, 240-243 (2012). Report of the use of silver-thiolate chromatographic material as a stable material for TLC. The stationary phase operated under the same principles as silver-ion chromatography, separating compounds by degree of unsaturation; however, showed considerable advantages over Ag-TLC in terms of light stability and shelf lifetime. Demonstration of the light stability and its application for separations based on the degrees of unsaturation. TLC of using fatty acid methyl esters (FAMEs) with hexane – ethyl acetate 9:1 and of polycyclic aromatic hydrocarbons (PAHs) with 100 % ethyl acetate.

Chromatogr Sci. 50 (3), 157-161 (2012). Study of the lipophilicity of 12 new synthesized derivatives, the first eight compounds have as a basic chemical structure aryliden–hydrazino-selenazoles and the second group of the three compounds belongs to aroyl–hydrazinoselenazoles. HPTLC on RP-18 with methanol–water mixtures in different ratios. The linear correlation between RMw and the methanol–water ratios showed high values for the correlation coefficient. Determination of the chromatographic hydrophobic index by using the ratio –RMw/S, giving the values ranged between 99 and 73, and a good linear correlation between RMw and the slope. The log P values are also calculated. Formation of the matrices with RMw and log P and a principal component analysis (PCA) was implemented. Extraction of the information from PCA by plotting the obtained matrices. The compounds can be grouped by analyzing the scores into one containing nine compounds, and other containing three compounds, each group of compounds with the same basic chemical structure.

Chinese J. of Veterinary Drugs 46 (5), 29-31 (2012). TCM is more and more important in veterinary medicine. It has been found that some medicines were illegally mixed with certain chemicals harmful to the animal body. Rapid screening of the additives illegally mixed in Astragalus polysaccharides injection by TLC on silica gel with 1) chloroform – methanol – acetone – ammonia 9:2:1:0.05 and 2) ethyl acetate – ethanol 1:1. Detection 1) under UV 254 nm; 2) by spraying with 5 % potassium iodobismuthate solution and evaluation in daylight; 3) by exposure to iodine vapor for a few minutes and evaluation under UV 254 nm. Identification of lincomycin, enrofloxacin, novalgin, 4-acetamino phenol, moroxydine hydrochloride, and ribavirin by comparison of the fingerprint with individual standards, with detection limits of 2.0, 0.5, 1.0, 0.5, 1.0, and 4.0 mg/mL, respectively. The method was applied to 25 batches of market samples and the results were compatible with those obtained by HPLC.

by thin-layer chromatography) (Chinese) Chinese J. Ethnomed. Ethnopharm.(3), 53-54 (2012) Melodinus fusiformis is a herbal TCM drug specially effective for curing rheumatism. TLC of the extractsand standard oleanolic acid of the crude drug on silica gel with n-hexane – ethyl acetate 7:3, detection by spraying with 5 % phosphomolybdate in n-propanol and heating using a air blower until the zones are visible.