Brazilian Journal of Pharmacognosy. 23, 207-216 (2013). HPTLC fingerprint of four similar looking Adiantum species: A. capillus-veneris L. (1), A. lunulatum Burm. f. (2), A. peruvianum Klotzsch (3), and A. venustum D. Don. (4) on silica gel with toluene - ethylacetate 4:1. (1) could be clearly differentiated from other species by the presence of characteristic blue colored bands at hRf 57 under UV 366 nm. (2) and (3) shared additional bands at hRf 22 and 27 under UV 366 nm and at hRf 74 under UV 254 nm. A characteristic band at hRf 41 was observed only in (3) under UV 366 nm.

Chinese J. of Xizang Sci. & Technol. 231, 48-49 (2012). Shanhu Qishiwan pills are a traditional Tibetan herbal medicine used for relieving uneasiness of mind and body, invigoration of blood circulation and relief of pain. For quality control, TLC on silica gel with n-hexane – ethyl acetate 4:1, detection under UV 366 nm.

determination of log P of (E)-3-benzylidene-2,3-dihydro-1-benzopyran-4-ones by RP-TLC. Effect on log P of incorporation of oxygen atom into carbocyclic chalcone analogues. J. Planar Chromatogr. 26, 284-288 (2013). Log P-TLC of (E)-3-benzylidene-2,3-dihydro-1-benzopyran-4-ones on silica gel with methanol - water 3:2. Detection under UV light at 254 nm. The effect of positions (ortho, meta, and para) of the aromatic substituents in the compounds was evaluated.

J. Planar Chromatogr. 26, 508-509 (2013). TLC of endosulfan on silica gel with n-hexane - acetone 4:1. Detection by spraying with sodium hydroxide followed by fuchsin (1) or malachite green (2) reagents (50 mg in 100 mL water), followed by exposure to bromine gas for 3-5 min. The hRf values of endosulfan detected by (1) and (2) were 50 and 80, respectively.

Chinese J. Mod. Drug Appl. 7 (17), 228-229 (2013). Zhuangyao Zhitong Heji compound oral liquid is a herbal TCM preparation to treat deficiency of the kidney and liver, chills and pain of the waist and knee, wind-cold-dampness arthralgia and low back injury. For quality control, TLC on silica gel with n-hexane – ethyl acetate 9:1, detection under UV 366 nm. Identification of Angelica sinensis, Ligusticum chuanxiong and Saposhnikovia divaricata roots by fingerprint comparison with reference drugs.

Chinese J. of Guide for Trad. Chinese Med. & Pharm. 2 (2), 97-99 (2013). Sanhuang zhiyang chaji liniment is a herbal TCM preparation prescribed for treating skin itching, carbuncle furuncle, acute eczema, impetigo and skin infection. For quality control, TLC on silica gel 1) for Cortex Phellodendri Chinensis and the standard berberine hydrochloride, with toluene – ethyl acetate – isopropanol – methanol – ammonia 12:6:3:1:1, detection by exposure to ammonia vapors and viewing under UV 366 nm; 2) for Radix Scutellariae and the standard baicalin, with ethyl acetate – butanone – methanol – water 5:3:1:1, detection by spaying with 1 % ferric chloride in ethanol and viewing in daylight; 3) for Radix et Rhizoma Rhei and the standard emodin, with petroleum ether (60-90 °C) – ethyl formate – formic acid 15:5:1, detection under UV 366 nm; 4) for Radix Sophorae Flavescentis and the standard matrine, with benzene – propanol – ethyl acetate – ammonia 10:15:20:1, detection by spaying with 5 % potassium iodobismuthate in hydrochloric acid – water 1:200 and viewing in daylight.

Chinese J. Econ. Forest Res. 31 (2), 142-145 (2013). Compared to other analytical techniques TLC has the advantage of no/minor sample pretreatment, simultaneous separation and determination of numerous and complex samples. Determination of polypeptide in protolysate of Camellia oleifera cake by TLC on silica gel with n-butanol – ethanol – water 4:1:1, detection by spraying with 0.5 % ninhydrin in propanone and heating at 105 °C for 15 min and viewing in daylight. Quantification of polypeptide by densitometry at 231 nm using glutathione (a tripeptide composed of glutamic acid, cysteine and glycine) as the external calibration standard. The %RSD for repeatability was 0.03 % (n=5). Recovery by standard addition was 106.9 % (%RSD=0.06 %, n=5). The LOQ was 2 µg/zone. The results of the TLC method for a batch of real life samples were comparable with the results by RP-HPLC.

J. Planar Chromatogr. 27, 97-101 (2014). HPTLC of myristicin (1) and linalool (2) in the leaf extracts of parsley, dill and celery on silica gel with petroleum ether - dichloromethane 3:7. Detection by spraying with vanillin reagent, followed by heating at 120 ºC for 5 min. Quantitative determination by absorbance measurement at 600 nm. Linearity was in the range of 5-21 μg/zone for (1) and 1-6 μg/zone for (2). The intermediate/interday/intra-day precisions were below 3 % (n=6). The LOD and LOQ were 1030 and 2000 ng/zone for (1) and 430 and 830 ng/zone for (2), respectively.