J. Planar Chromatogr. 29, 281-286 (2016). HPTLC of newly synthesized cycloalkylspiro-5-hydantoins (derivates of 3-(4-substituted benzyl)-cyclopentanespiro-5-hydantoin, 3-(4-substituted benzyl)-cyclohexanespiro-5-hydantoin, and 3-(4-substituted benzyl)-_x000D_cycloheptanespiro-5-hydantoin) on RP-18 with varying content of methanol 50–90%. Multivariate analysis enabled the classification of the lipophilicity and structural properties of the investigated compounds.

J. Liq. Chromatogr. Relat. Technol. 39, 303-307 (2016). HPTLC fingerprinting of 27 white wines of three sorts on silica gel with ethyl acetate – formic acid – acetic acid – water 10:1:1:2 and on RP-18 with methanol – water – formic acid 50:50:1. Detection by heating at 100 °C for 3 min, followed by dipping into Natural Product reagent (1 g of diphenylborinic acid aminoethylester dissolved in 200 mL ethyl acetate) and after drying by dipping into PEG reagent (10 g of polyethylene glycol 400 dissolved in 200 mL dichloromethane). Qualitative identification under UV 254 nm and UV 366 nm.

Pharm. Res. 33, 1587-1601 (2016). HPTLC study of the cleavability of the disulfide bond in a conjugate siRNA-S-S-PE (incubated in 10 mM glutathione at 37 °C for 4 h) on silica gel with chloroform – methanol 4:1. Detection by spraying with molybdenum blue dye for the cleaved phospholipids. This test confirmed the potential utility of this system for siRNA delivery in vivo.

Polimeros. 26, 74-80 (2016). TLC of additives such as paraffinic oil (1), stearic acid (2), 2-mercaptobenzothiazole (3), and tetramethylthiuram disulfide (4) in rubber compositions of ethylene-propylene-diene rubber (EPDM) on silica gel with hexane – diethyl ether – acetic acid 14:6:1. After developing, the plate was heated at 105 ºC for 30 min and then cooled at room temperature. Detection by spraying with 1 % solution of 2,6-di-chloro-para-chloro benzoquinine imine in ethanol. The hRF values for (1) to (4) were 10, 65, 37 and 14, respectively.

J. Planar Chromatogr. 29, 306-309 (2016). HPTLC of some red, rose, and white wines on silica gel with ethyl acetate – acetic acid – formic acid – water 10:1:1:2. Antioxidant activity determination by dipping into a 0.04 M methanolic 2,2-diphenyl-1-picrylhydrazyl (DPPH radical reagent) solution (1) and into a 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) solution (2; prepared by mixing 7 mM ABTS diammonium salt solution with 2.45 mM potassium persulfate solution 1:1). The plates were then dried in the fume hood and stored in the dark. The antioxidant activities were determined after 10 and 30 min reaction time. Method (2) was more sensitive than the DPPH assay.

J. Planar Chromatogr. 29, 330-335 (2016). HPTLC of wedelactone in Eclipta alba, Wedelia calendulacea and Wedelia trilobata on silica gel with toluene – chloroform – ethanol – formic acid 10:8:2:1. Quantitative determination by absorbance measurement at 351 nm. The hRF value for wedelactone was 29. Linearity was between 80 and 280 ng/zone. The intermediate precision was below 2.5 % (n=3). The LOD and LOQ was 0.36 and 1.09 ng/zone, respectively. Recoveries were between 99.4 and 100.3 %.

J. Planar Chromatogr. 29, 388-390 (2016). HPTLC of dimethoate from post mortem viscera on silica gel with hexane - acetone 4:1. Detection by spraying with freshly prepared sodium nitroprusside solution (1 % sodium nitroprusside in 2 N sodium hydroxide solution prepared with especially sulfide-free distilled water). The hRF value for dimethoate was 88.

J. Planar Chromatogr. 30, 154-163 (2017). HPTLC of chlorpyrifos (1), quinalphos_x000D_ (2), and triazophos (3) on silica gel with n-hexane ‒ acetone 9:1 and on RP-18 with acetonitrile ‒ water 4:1. The results indicated that the hRf values of all three pesticides increased with the polarity of the solvent.