Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
  • Browse and search by CBS classification: Select one of the 38 CBS classification categories where you want to search by a keyword
  • Keyword register: select an initial character and browse associated keywords
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      118 076
      Thin-layer chromatography of green leaves extracts - zinc migrates into pheophytin on TLC silica plates with fluorescence indicator (F254)
      Birte Johanne SJURSNES, Lise KVITTINGEN*, R. SCHMID (*The Norwegian University of Science and Technology (NTNU), 7491 Trondheim, Norway, lise.kvittingen@ntnu.no)

      J. Planar Chromatogr. 29, 480-483 (2016). 2D-TLC of pheophytins in green leaves on silica gel with hexane ‒ acetone ‒ ethanol 35:14:1. An analogous transverse elution was undertaken after 5 min and 1, 3, 7, and 9 days. The paper suggests that the green colored spots on the silica gel layer with fluorescence indicator are due to the incorporation of Zn2+ in the compounds.

      Classification: 30b
      119 019
      Pressurized planar electrochromatography as a supporting tool for qualitative toxicological chemical analysis with thin-layer chromatography and UV-Vis spectrometry
      T. DZIDO*, E. KOPCIAL, J. SZPONAR, A. KRAJEWSKA, L. AFTYKA, J. BAKIERA, B. POLAK, A. KLIMEK (*Department of Physical Chemistry, Faculty of Pharmacy with Medical Analytics Division, Medical University of Lublin, Lublin, Poland, tadeusz.dzido@umlub.pl)

      J. Liq. Chromatogr. Relat. Technol. 40, 320-326 (2017). HPTLC of 31 substances of toxicological interest on RP-18 with 60 % acetonitrile in buffer pH 6.0, on silica gel with chloroform – methanol 9:1 and using pressurized planar electrochromatography (PPEC) on RP-18 with 60 % acetonitrile in buffer pH 2.2. The authors showed the application of the migration distance of the solutes, obtained by the PPEC technique, with a proposed equation allowed for increase of likely identification of substances in toxicological analysis.

      Classification: 3g, 32d
      119 069
      Identification of enzymes involved in the mevalonate pathway of Flavobacterium johnsoniae
      H. HAYAKAWA, F. SOBUE, K. MOTOYAMA, T. YOSHIMURA, H. HEMMI* (*Department of Applied Molecular Bioscience, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan, hhemmi@agr.nagoya-u.ac.jp)

      Biochem. Biophys. Res. Commun. 487, 702-708 (2017). HPTLC of the reaction products of different 14C-labeled substrates of the mevalonate pathway (MVA, MVA-5-P, MVA-3-P, MVA-5-PP) and the recombinant Flavobacterium johnsoniae protein on silica gel with n-propanol – 28 % ammonia – water 6:3:1. The distribution of radioactivity on the TLC plate was detected using a suitable image analyzer.

      Classification: 18b, 34
      119 106
      TLC bioautographic method for activity screening of natural acetyl cholinesterase inhibitors obtained from Xinjiang Mohe tobacco
      J. WANG (Wang Jian), P. ZHU (Zhu Pingping), G. NI (Ni Guozhu), L. WEN (Wen Linyu), L. ZHI (Zhi Ling), SH. HU (Hu Shuchen)* (*Xinjiang Med. Univ., Xinjiang, Wulumuqi 830011, China, ansenl224@163.com)

      J. of China Pharm. 23 (20), 38-40 (2014). Nicotine is a toxic substance in tobacco. Studies have shown that nicotine may prevent and treat Alzheimer's disease (AD) and Parkinson's syndrome (PD). At present, the most effective method to treat AD is by inhibiting the activity of acetylcholinesterase (AChE), thus enhancing the cholinergic activity indirectly. In order to clarify the mechanism of nicotine reducing the incidence of AD, the activity of natural AChE inhibitors in Xinjiang Mohe tobacco was screened by TLC on silica gel with (A) ethyl acetate – methanol – ammonium hydroxide 70:30:1, followed by detection via enzyme inhibition (this is not bioautography, as stated in the title): firstly by spraying with 1.0 U/mL AChE (AChE 500 U + tris-HCl buffer solution of pH 7.8 500 mL + bovine serum albumin 500 mg), followed with 1.5 g/L alpha-naphthyl acetate (150 mg in ethanol 40 mL + water 60 mL) and then with 0.5 g/L Fast blue B salt, resulting in white zones on purple background; (B) toluene – ethyl acetate - diethylamine 7:2:1, detection by spraying with 0.2 mmol/L 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical reagent, resulting in white to yellow zones on pale purple background. The results indicated that nicotine in Xinjiang Mohe tobacco could bind to AChE and thus inhibit its activity.

      Classification: 32b, 32e
      120 023
      High performance thin-layer chromatography – mass spectrometry enables reliable analysis of physalins in different plant parts of Physalis alkekengi L
      E. KRANJC, A. ALBREHT, Irena VOVK*, V. GLAVNIK (*Dep. of Food Chem., Nat. Inst. of Chem., Hajdrihova 19, 1000 Ljubljana, Slovenia, irena.vovk@ki.si)

      J. Chromatogr. A 1526, 137-150 (2017). HPTLC of physalins from crude extracts of Chinese lantern (Physalis alkekengi L.) on silica gel with ethyl acetate – toluene – formic acid 35:15:1. Densitometric screening of physalins in absorption and in fluorescence mode after post-chromatographic derivatization with sulfuric acid reagent. Identification of the physalin L standard and its impurity, 2,3,25,27-tetrahydrophysalin A. Applying two successive plate pre-developments with methanol – formic acid 9:1 and methanol to avoid strong ion suppression caused by the developing solvent additive (formic acid), and to improve the sensitivity of HPTLC-MS/MS method combined with a slightly modified developing solvent ethyl acetate – toluene – formic acid 30:20:1. Non-targeted characterization of physalins from the same chromatographic zone and determination of physalin types by simultaneous hyphenation of HPTLC with a triple quadrupole and an ion trap mass analyzer. Demonstration of the performance of the HPTLC-densitometric and HPTLC–MS/MS methods for the analysis of physalins from aqueous reflux extracts. Observation of variations in physalin profiles and abundances in different parts of P. alkekengi harvested at different stages of maturity, showed that the husks are the most suitable plant part for P. alkekengi quality control.

      Classification: 4e, 32e
      120 059
      Is equol an ecologically relevant endocrine disruptor? Equol determination in cattle manure by effect-directed analysis using the pYES test
      Y.A. MINAR, B. MILZ, B. SPANGENBERG* (*University of Offenburg, Department of Process Engineering, Badstrasse 24, 77652 Offenburg, Germany, Spangenberg@HS-Offenburg.de)

      J. Planar Chromatogr. 31, 72-78 (2018). HPTLC of equol in ethanolic cattle manure extract on RP-18 with n-hexane – ethyl – acetate – acetone 9:3:2. Detection by planar yeast estrogen screening (pYES) by dipping into a yeast suspension, followed by incubation at 30 °C for 4 h. After incubation, the plate was dried in a 37 °C incubator for 15 min and dipped into the combined reaction buffer followed by incubation at 37 °C for 60 min and 90 % relative humidity. The combined reaction buffer was prepared by mixing 20 mL of buffer C (5.3 g of sodium phosphate dibasic and 0.4 g of potassium chloride were dissolved in about 490 mL water, the solution was adjusted with sodium hydroxide to pH 13, 0.5 g of benzalkonium chloride were added and the mixture was filled up to 500 mL) and 0.2 mL of a freshly prepared X-Gal solution (0.05 g/mL X-Gal in DMSO). Fluorescence evaluation under UV 366 nm. The hRF value for equol was 47.

      Classification: 13b
      120 084
      St. John’s wort versus counterfeit St John’s wort – an HPTLC study
      Debora FROMMENWILER*, E. REICH, S. SUDBERG, M. SHARAF, A. BZHELYANSKY, B. LUCAS (*CAMAG, Sonnenmattstrasse 11, 4132 Muttenz, Switzerland, debora.frommenwiler@camag.com)
      J. AOAC Int. 99, 1204-1212 (2016). HPTLC of St. John’s wort labeled as Hypericum perforatum on silica gel with ethyl acetate – dichloromethane – formic acid – acetic acid – water 100:25:10:10:11 (USP 38-NF33), ethyl acetate – formic acid – water 30:2:3 (European Pharmacopoeia) and ethyl acetate – acetic acid – formic acid – water 100:11:11:26 (USP 37-NF32). For the investigation of synthetic dyes, samples were analyzed on RP-18 with methanol – 5 % aqueous sodium sulfate 3:4. Detection by dipping into natural products reagent (1 g diphenylborinic acid 2-aminoethylester in 200 mL ethyl acetate), then in polyethylene glycol reagent (10 g PEG 400 (macrogol) in 200 mL dichloromethane). Qualitative identification under UV 254 and 366 nm. A decision tree was developed to analyze adulterated samples labeled as Hypericum perforatum. If for example under UV 366 nm after derivatization a yellow fluorescent zone is seen at hRF 46, samples are probably mixed with another undesired species. In addition, if a bluish zone is seen at application position of samples identified with the test USP 38-NF32 and examined under white light prior derivatization, the sample is probably mixed with dyes.
      Classification: 32e
      121 035
      A multi-methodological approach in the study of Italian PDO "Cornetto di Pontecorvo" red sweet pepper
      A. SOBOLEV, Luisa MANNINA*, D. CAPITANI, G. SANZO, C. INGALLINA, B. BOTTA, S. FORNARINI, M. CRESTONI, B. CHIAVARINO, S. CARRADORI, M. LOCATELLI, A. GIUSTI, G. SIMONETTI, G. VINCI, R. PRETI, C. TONIOLO, M. REVERBERI, M. SCARPARI, A. PARRONI, L. ABETE, F. NATELLA, A. DI SOTTO (*Dipartimento di Chimica e Tecnologie del Farmaco, Sapienza Università di Rome, P. le Aldo Moro 5, 00185 Rome, Italy, luisa.mannina@uniroma1.it)

      Food Chem. 255, 120-131 (2018). HPTLC of polyphenols (chlorogenic acid and rutin) in the peel, pulp, and the edible part of pepper on silica gel with ethyl acetate – dichloromethane – formic acid – acetic acid – water 100:25:10:10:11. Detection by spraying with Natural Product reagent and anisaldehyde sulfuric acid solution. Qualitative identification under UV 254 and 366 nm.

      Classification: 7
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