Microbiol. Res. 186-187, 119-131 (2016). TLC bioautographic analysis of active extracts of the producing strain Natrinema gari QI1 on silica gel with n-butanol – acetic acid – water 3:1:1. The agar media containing the indicator strain Haloferax mediterranei SAG3 was applied directly onto the developed TLC plate and incubated at 40 °C for 7 days. The compound with the most significant inhibition of Haloferax mediterranei was at hRF 29.

J. Planar Chromatogr. 30, 219-224 (2017). HPTLC of 18 derivatives obtained by substitution of chlorine atom at C7 or/and C6 in 6,7-dichloro-5,8-quinolinedione on RP-18 with a mixture of acetone and _x000D_water solution of buffer Tris (pH 7.4) in different volume compositions ranged from 30 % to 85 % in 5 % increments. The correlation between experimental and calculated values of lipophilicity was analyzed.

J. Planar Chromatogr. 30, 121-125 (2017). Overpressured TLC of essential oil components of clove, rosemary, eucalyptus, tea tree, spearmint, thyme, and cinnamon on silica gel with toluene. The conditions in infusion mode were as follows: 50 bar, external pressure; 350 μL, rapid mobile phase flush; 500 μL/min, mobile phase flow rate; 3900 μL, mobile phase; 475 s, development time. The hRF values were 8 for α-terpineol, 12 for borneol, 17 for terpinen-4-ol, 20 for 1,8-cineole, 22 for R(–)-carvone, 31 for trans-cinnamaldehyde, 36 for eugenol and 43 for thymol. Antibacterial and antioxidant activities were also detected by infusion–transfusion OPLC hyphenated with Aliivibrio fischeri assay and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical assay, respectively.

J. Chromatogr. Sci. 54 (4), 609-617 (2016). HPTLC of sulbutiamine (SUL) in the presence of different degradation products after subjecting the drug to stress conditions (according to ICH: neutral, alkaline and acidic hydrolysis, oxidation, photodegradation and dry heat degradation), on silica gel aluminum foil with acetone – methylene chloride – ammonia buffer (pH 8.5±0.2) 14:6:1. Densitometric evaluation at 254 nm. The calibration curve was between 0.4–5.0 µg/zone with good correlation coefficients. The LOD and LOQ were 110 and 330 ng/zone, respectively. Structure elucidation of the resulting degradation products by ESI-MS/MS. The results showed that the drug was completely degraded with 0.1 N NaOH, 1 N HCl and 30 % hydrogen peroxide, while it was partially degraded by 0.1 N HCl, 3 % hydrogen peroxide and UV light. The hRf of SUL was 46 and the zone was completely separated from all obtained degradation products.

J. Chromatogr. A 1515, 232-244 (2017). Characterization of lipids in both bone marrow (BM) and mineralized tissue (MT) compartments, and their potential implication in bone pathologies, involving sample preparation, lipid extraction and analytical issues using a small sample size (≤ 0.5 g of rat femurs). Two major issues in bone handling were addressed with two cleaning steps after BM removal and by adding a demineralization step to the overall lipid extraction protocol, to avoid potential contamination of the MT by marrow lipids and the poor accessibility of certain lipids from the MT. HPTLC of the major neutral and polar lipids provided excellent resolution for 15 standards, good precision (inter-day %RSD <13 %) and recoveries of the standards ranged between 76 and 122 %. The method was suitable for lipid determination in both BM and MT and reliable in terms of lipid quantification. Demineralization facilitates phosphatidylserine and cholesterol ester extractions from the MT. Confirmation of the HPTLC data by HPLC determination of fatty acids as naphthacyl esters in bone samples. The mineralized tissue seems to be more metabolically active than the bone marrow.

J. Planar Chromatogr. 30, 363-374 (2017). HPTLC of acetylsalicylic acid (1) and its related compound salicylic acid (2) on silica gel with n-hexane – diethyl ether – 80 % acetic acid 7:2:1. Good quality densitograms with well separated and symmetric peaks of (1) and (2) were achieved. Detection with the following reagents: Janus blue, bromophenol blue, bromocresol blue, hydrogen peroxide (without heating and with heating to 90 °C during 60 min), 1 % sodium hydroxide (with heating to 45 °C and also with heating to 90 °C during 60 min), brilliant green, malachite green, and thymol blue.

Phytochem. Anal. 28, 125-131 (2017). HPTLC of the dried fractions of Morus alba wood on silica gel with dichloromethane – methanol 22:3, and to achieve better resolution for the high activity fractions with dichloromethane – methanol 83:17. Qualitative determination under UV 254 and 366 nm and detection with sulfuric acid reagent. Partial least squares-regression was performed to discover the substances that were most correlated to bioactivity.

J. Liq. Chromatogr. Relat. Technol. 41, 315-323 (2018). HPTLC of three types of macrocyclic compounds, i.e. (1) cyclodextrins, (2) calixarenes and (3) macrocyclic antibiotics, on silica gel or RP-18 with different compositions of water – organic liquids (methanol, ethanol, acetonitrile). Detection of (1) on silica gel with a 1:1 mixture of 10 % α-naphthol in ethanol and 10 % sulfuric acid in water, followed by heating at 120 °C for 5-10 min; and on RP18 W by spraying with a 25 % methanolic solution of sulfuric acid, followed by heating at 120 °C for 5-10 min. Detection of (2) by spraying with 20 % aqueous solution of perchloric acid, followed by heating at 120 °C for 5 min. A comprehensive retention data set was obtained for fast selection of mobile phase compositions.