J. Chinese Trad. Patent Med. (Zhongchengyao) 11, 885-887 (2004). TLC on silica gel with 1) n-butanol - 3 M NH4OH - ethanol 5:2:1, 2) toluene - ethyl acetate - formic acid 5:4:1. Detection 1) under UV 254 nm, 2) by spraying with 10 % FeCI3 in ethanol. Identification by fingerprint techniques. Quantitative determination of quercetin by HPLC.

J. Planar Chromatogr. 17, 149-153 (2004). TLC on silica gel and RP-18 after pre-saturation with methanol - water, ethanol - water, propanol - water, acetonitrile - water, acetonitrile - methanol, THF - water, acetonitrile - buffer, and methanol - buffer in which the concentration of organic modifier was varied from 0 to 100 %. Detection by spraying with conc. sulfuric acid - methanol 1:4 followed by heating for 10 min at 120 °C and illumination with UV light.

J. Pharm. Biomed. Anal. 34, 525-530 (2004). HPTLC of busulfan, an alkylating agent in pharmaceutical preparations, on Lichrospher silica gel with ethyl acetate - chloroform - methanol 13:4:3 in horizontal sandwich chamber. After development the plate was soaked in 2 % ethanolic solution of 4-nitro benzyl pyridine, followed by heating at 193 °C for 10 min. Quantitative determination by absorbance measurement at 600 nm. The method was validated for accuracy, precision, linearity (100-500 µg/mL), and specificity. The method was suitable for stability studies of busalfan in pharmaceutical preparations.

Indian Drugs 41 (8), 482-487 (2004). HPTLC on silica gel with toluene - acetone - ammonia 50:50:1. Quantitative determination by absorbance measurement at 315 nm. The method was validated in terms of linearity (300-1000 ng/spot), precision, accuracy, and specificity. For the content uniformity test the tizanidine hydrochloride content of 10 individual tablet units of two market formulations was determined after extracting with methanol. Both formulations compiled with the USP specification. The proposed content uniformity allows the parallel analysis of ten tablets on a single plate and provides a faster and cost-effective quality control tool for routine analysis. A simple, sensitive HPTLC content uniformity test was developed and validated for the analysis of tizanidine hydrochloride in its commercial single component tablet formulations (2 mg/tablet).

Chinese J. Trad. Patent Med. (Zhongchengyao) 26 (4), 341-343 (2004). TLC of Changchun Hongyao capsule extracts on silica gel with n-hexane - ethyl acetate 1:1. Detection by spraying with 5 % solution of potassium iodobismuthate. Identification by fingerprint techniques. Quantification of aconitine by HPLC.

J. Planar Chromatogr. 17, 388-390 (2004). HPTLC of uroporphyrins I and III, coproporphyrins I and III, and protoporphyrin on silica gel with binary mobile phases prepared by mixing pure esters, ketones, and xylenes with DMSO in proportions from 0 to 100 %. Detection under UV light at 254 nm. The separation of the porphyrins required the presence of DMSO in the mobile phase.

Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (9), 716-719 (2004). TLC on silica gel with 1) ethyl acetate - chloroform - formic acid 3:2:1; 2) chloroform - methanol 7:2; 3) petroleum ether (60-90 ºC) - ethyl acetate 7:3. Detection 1) under UV 365 nm; 2) by spraying with 10 % H2SO4 in ethanol and heating at 105 ºC for 5 min; 3) by spraying with vanillin - H2SO4 solution. Identification by fingerprint technique. Quantification of icarrin by HPLC.

Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (9), App.14-16 (2004). TLC on silica gel with 1) toluene - ethyl acetate - formic acid 25:20:4; 2) ethyl acetate - methanol - water 100:17:13. Detection 1) by spraying with diazotized para-nitroaniline solution; 2) by spraying with 5 % AlCl3 in ethanol and under UV 365 nm. Identification by fingerprint technique. Quantification of hesperidin by HPLC.