J. Chinese Trad. Patent Med. (Zhongchengyao) 25 (8), 680-682 (2004). TLC on silica gel with 1) ethyl acetate - formic acid - water - methanol 35:10:15:2; 2) benzene - ethyl acetate 19:1; 3) chloroform - ethyl acetate - methanol - water; 4) petroleum ether; 5) petroleum ether - ethyl acetate 17:3; 6) chloroform - methanol - water 13:7:2; 7) ethyl acetate - formic acid - water 10:2:3. Detection 1) under daylight, 2) by spraying with 5 % H2SO4 in ethanol and heating at 105 ºC, 3) by spraying with 5 % p-dimethylaminobenzaldehyde in 10 % H2SO4 in ethanol and heating at 105 ºC, 4) by spraying with 2 % 2,4-dinitrophenylhydrazine in ethanol, 5) by spraying with 1 % vanillin - H2SO4 solution, 6) by spraying with 5 % FeCI3 in ethanol, 7) by spraying with 2 % AICI3 in ethanol and under UV 365 nm, 8) under UV light. Identification by fingerprint techniques.

J. Planar Chromatogr. 17, 143-146 (2004). TLC of oleic, elaidic, palmitic, stearic, ricinoleic acid, methyl ricinoleate, alpha-hydroxypalmitic acid, methyl alpha-hydroxypalmitate, 12-hydroxystearic acid, methyl 12-hydroxystearate, 9,10-dihydroxystearic acid, methyl 9,10-dihydroxystearate on RP-18 with methanol - water 19:1 and 9:1. Detection with iodine vapor.

J. Liq. Chrom. Rel. Technol. 27, 2087-2100 (2004). HPTLC of tetrandrine on silica gel with toluene - ethyl acetate - methanol - 28 % ammonia 100:100:50:3. Detection under UV light at 366 nm, and under white light after reaction with iodine (yellowish zones). If the plate is subsequently derivatized with anisaldehyde solution, the alkaloids show a strong blue fluorescence under UV light at 366 nm. Quantitative determination at 210 nm. The calibration curve is linear for 50 - 112.5 ng tetrandine per zone. Also HPTLC of aristolochic acids (AAs) on silica gel with the upper phase of toluene - ethyl acetate - water - formic acid 20:10:1:1 and derivatization with tin(II) chloride (to be prepared freshly: dissolve 1 g tin(II) chloride•2H2O in 1.5 mL 36 % hydrochloric acid diluted with 8 mL water), followed by heating at 100 °C for 1 min and evaluation under UV light at 366 nm. The method allows detection of 1 ppm of AA.

Indian J. Pharm. Sci. 66 (3), 283-286 (2004). TLC of lamotrigine in its tablet dosage form on silica gel with acetone - toluene - ammonia 14:6:1. Quantitative determination by absorbance measurement at 312 nm. The method was quantitatively evaluated in terms of linearity, accuracy, precision, repeatability, and specificity to prove its utility in the analysis of tablet dosage form.

Chinese J. Trad. Patent Med. (Zhongchengyao) 26 (7), 597-600 (2004). TLC of Qieban Zhiyang ointment extracts on silica gel with 1) benzene - ethyl acetate 10:1; 2) petroleum ether (60-90 ºC) - ethyl formate - formic acid 15:5:1; 3) chloroform - methanol - ammonia 40:3:1; 4) benzene - chloroform - acetone 5:4:1; 5) chloroform - methanol 19:1. Detection 1) under UV 365 nm; 2) by exposing to ammonia vapor; 3) by spraying with potassium iodobismuthate solution; 4) under daylight. Identification by fingerprint technique. Quantification of icariine by HPLC.

J. Planar Chromatogr. 17, 255-260 (2004). TLC of fourteen 1-arylpiperazine derivatives on silica gel with monocomponent and binary non-polar mobile phases and on RP-18 with binary mobile phases comprising mixtures of methanol, acetone, or dioxane (as organic modifiers) and water in a horizontal chamber after equilibration for 15 min. Detection under UV light at 254 nm.

Chinese J. Trad. Pat. Med. (Zhongchengyao) 27(1), 94-96 (2005). HPTLC on silica gel with 1) n-butanol - glacial acid - water 19:5:5; 2) petroleum ether (30-60 ºC) - formic acetate - formic acid 15:5:1; 3) chloroform - ethyl acetate - methanol - formic acid 200:25:50:1. Detection 1) by spraying with 0.5 % ninhydrin in ethanol; 2) under UV 365 nm; 3) by spraying with vanillin - H2SO4 solution and heating. Identification by fingerprint technique. Quantification of emodin by densitometry at 440 nm. Validation of the method by investigation of linearity (0.1 µg - 0.5 µg, r = 0.998); precision (RSD = 3.8 %, n = 15 within plate and RSD = 3.2 %, n = 5 plate to plate); reproducibility of five time assay towards the same sample (RSD = 4.4 %); standard addition recovery (99.5 %, RSD = 2.2 %, n = 5). The results for some real life samples are given.

Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (9), App.12-14 (2004). TLC on silica gel with 1) n-butanol - glacial - water 19:5:5; 2) chloroform - methanol - glacial acetic acid - 17:2:1; 3) two fold development with benzene - acetone 9:1. Detection 1) by spraying with ninhydrin solution and heating at 105 ºC; 2) by spraying with 5 % vanillin solution and heating; 3) by spraying with a solution of 8 % vanillin in ethanol - H2SO4, and heating at 105 ºC. Identification by fingerprint technique. Quantification of phyllyrin by densitometry at 280 nm. The quantitative procedure is validated by investigating its linearity (1 - 5 µg/spot, r = 0.9998); precision (RSD = 0.36 % n = 5); repeatability (RSD = 2.92 % n = 5) and standard addition recovery (99.6 %, RSD = 2.4 %, n = 5), etc. The determination results are given for a group of real life samples.