Rev. Fac. Agron. 21, 155-160 (2004). HPTLC of betaxantin and betacyanin in the roots of Beta vulgaris on cellulose in two one-dimensional developments with 1) isopropanol – ethanol – water – acetic acid 6:7:6:1 and 2) isopropanol – ethanol – water – acetic acid 11:4:4:1. Qualitative identification under UV light. Betaxantin and betacyanin showed maximum absorbances at 537 and 465 nm, respectively. The hRf values of betaxantin and betacyanin were 22 and 34, respectively.

Anal. Chim. Acta 598 (2), 312-317 (2007). TLC of paroxetine hydrochloride on silica gel with butanol - acetic acid - water 16:4:1. The hRf value of paroxetine HCl was 48 and separation from the degradation products (produced by acid and alkali hydrolysis, oxidation and photodegradation) was good. Quantitative determination by absorbance measurement at 295 nm. The linearity was in the range of 300 - 1500 ng/spot and the correlation coefficient was 0.9903. The limit of detection and quantitation was 50 and 150 ng/zone, respectively.

Phytochem. Anal. 20, 511-515 (2009). Bioautography of alpha-D-glucosidase (1) and beta-D-glucosidase (2) in buffer solution (sodium acetate 4.1 % in water pH=7.5) sprayed onto a silica gel plate. Incubation at room temperature for 60 min for (1) and at 37 °C for 20 min for (2). For detection of the active enzyme, solutions of 2-naphthyl-alpha-D-glucopyranoside or 2-naphthyl-beta-D-glucopyranoside and Fast Blue salt were mixed at a ratio of 1:1 for (1) or 1:4 for (2), and sprayed onto the plate to give a purple background coloration after 2-5 min. Methanol extracts of the aerial parts of Tussilago farfara and Urtica dioica were tested as enzyme inhibitors and visualized as white spots on the TLC plates.

J. Chromatogr. B 877 (29), 3719-3723 (2009). HPTLC of gemifloxacin mesylate in human plasma, extracted with chloroform - acetic acid 59:1, on silica gel with ethyl acetate – methanol - ammonia 8:4:3. The hRf value of gemifloxacin mesylate was 33. Quantification by densitometry at 254 nm. The calibration curve was established in the range of 50 to 600 ng/spot. Recovery of gemifloxacin mesylate was between 80.0 and 86.2 %. The stability of gemifloxacin mesylate in plasma was confirmed with samples submitted to three cycles of freeze–thawing at -20 °C, and with samples stored on the bench for 12 h.

J. Chinese Pharm. Standard 10 (4), 284-286 (2009). TLC of Naolibao pill extracts on silica with n-hexane - ethyl acetate 5:1. Qualitative identification by detection under UV 254 nm and 365 nm. The method is simple, rapid, reliable, and suitable for the quality control of the TCM formulation.

J. Chromatogr. Sci. 48 (2), 109-113 (2010). HPTLC of nebivolol hydrochloride on silica gel with toluene – methanol - triethylamine 19:6:1. The hRf value of nebivolol hydrochloride was 33. Quantification by densitometry in the absorbance mode at 281 nm. Linearity was between 500 and 3000 ng/spot with r2= 0.9994. The limit of detection and quantification was 63 and 191 ng/spot, respectively. Nebivolol hydrochloride was subjected to acid and alkali hydrolysis, oxidation, thermal degradation, and photodegradation. The degradation products were well-resolved from the main component.

J. Planar Chromatogr. 23, 198-200 (2010). Proposition of new methods for calculation of the partition coefficients of aliphatic compounds from experimental Rf values and the numerical values of selected topological indexes. The experimental partition coefficient (log Pexp) of cetyl alcohol was determined for the n-octanol-water system. Numerical values obtained were compared with theoretical values from a database (AlogPs, AC_logP, AB/LogP, ALOGP, milogP, and XLOGP2). HPTLC of cetyl alcohol, stearyl alcohol, palmitic acid, stearic acid, alpha-hydroxypalmitic acid, and 12-hydroxystearic acid on RP18 with methanol and with methanol - water 19:1 in a horizontal chamber at room temperature. Detection after visualization in iodine vapor.

Ind. J. Pharma. Science 71 61, 656-662 (2009). Medicated oils prepared both by Ayurvedic as well as modified process were evaluated for fingerprint profiling by HPLC and HPTLC. HPTLC of methanolic extracts of the oils on silica gel with chloroform methanol 9:1 and toluene - ethyl acetate 4:1 for general fingerprint profiling; with toluene - ethyl acetate - formic acid 5:4:1 for flavonoids and with toluene - ethyl acetate - diethyl amine 7:2:1 for alkaloids. Evaluation under 254 nm and 365 nm. Detection by treatment with NP-PEG reagent for flavonoids and Dragendorff reagent for alkaloids.