J. of Qilu Med. & Pharm. 31 (2), 75-78 (2012). Pudilan Xiaojan oral liquid is a herbal TCM preparation effective in clearing heat and removing toxicity, because of its antiinflammatory activity it is used to cure swelling caused by furuncles, pharyngitis, lymphadenitis and tonsillitis. Dandelion is the key component drug of the preparation. Analysis of the components of dandelion in the preparation by TLC on silica gel with chloroform – methanol – formic acid 18:2:1, detection under UV 366 nm.

Chinese J. Ethnomed. Ethnopharm. (23), 70-71 (2011). Blood ginseng, the dried root of Indigofera stachyoides Lindl. is a traditional Chinese herbal crude drug with special effect in invigorating the circulation of blood, reducing phlegm, treating yin deficiency by reinforcing body fluid and nourishing the blood. It is used clinically to treat encephalalgia, woman’s abdominal pain, and metrorrhagia. For quality control 1) TLC of the extracts on silica gel with toluene – ethyl acetate – formic acid 6:3:1, detection in daylight; 2) determination of the moisture by drying in the oven; 3) determination of general ash content by firing, and of the acid insoluble ash content by acid pickling; 4) determination of ethanol extract by lixiviating with 50 % ethanol.

J. of China Pharm. 21 (12), 15-16 (2012). The leaf of Microcos paniculata L. is a popular medicinal herbal drug used for cardiovascular protection, blood fat adjusting, and anti-aging. Presentation of methods for identifying the drug by TLC and FTIR. TLC of the extracts of the drug on silica gel 1) for kaempferide, with toluene – ethyl acetate – formic acid 9:3:1, detection by spraying with 3 % aluminiumchloride in ethanol followed by heating at 105 ºC for 5 min and viewing at UV 366 nm; 2) for vitexin and isovitexin, developed with ethyl acetate – methanol – water 100:17:13, detection by spraying with 3 % aluminiumchloride in ethanol followed by heating at 105 ºC for 5 min and viewing at UV 366 nm. The methods have been applied to 15 batches of real life sample available from different places of origin, and proved that TLC procedures as well as FTIR are simple, sensitive, reproducible and robust and suitable for the identification of the herbal drug.

Chinese J. of Guide for Trad. Chinese Med. & Pharm. 18 (5), 80-81 (2012). Weijianning capsules, a herbal TCM preparation for tonifying spleen and invigorating the stomach, is prescribed clinically to treat superficial, erosive and atrophic gastritis. Presentation of a TLC method for the quality control of the preparations optimized by investigating the sample extraction procedure and the mobile phases employed. TLC of the extracts on silica gel 1) for Radix Scrophulariae with ethyl acetate – ethanol – water 20:5:3, detection by spraying with a solution of 3 g vanillin – 100 mL ethanol – 0.5 mL sulfuric acid and heating at 105 °C until the zones were clearly visualized, evaluation in daylight; 2) for Fructus Amomi with n-hexane - ethyl acetate 3:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones were clearly visualized, evaluation in daylight. Identification by fingerprint comparison in parallel with the individual standard components.

J. Planar Chromatogr. 26, 147-152 (2013). TLC of poly(methylmethacrylate) (PMMA) on silica gel with acetonitrile binary solvent mixtures with carbon tetrachloride, toluene, methylene chloride, chloroform and dichloroethane. Mark-Kuhn-Houwink parameters for PMMAs, Snyder’s parameters and the measured intrinsic viscosity values for PMMA. The characteristics of adsorption-exclusion transition depends both on thermodynamic affinity of the solvent for the polymer and macromolecule-to-pore size ratio.

J. Ethnopharmacol. 148, 869-879 (2013). HPTLC of cantharidin in the blister beetles Epicauta hirticornis and Mylabris cichorii on silica gel with ethyl acetate - chloroform. Identification by absorbance measurement at 254 nm. The hRf of cantharidin was 78 and structurally defined as hexahydro-3a,7a-dimethyl-4,7-epoxyisobenzofuran-1,3-dione.

Chinese J. of Lishizhen Trad. Med. & Pharm. 22 (5), 1045-1048 (2011). Dieda Zhitong microemulsion spray is a traditional Chinese medicine effective in invigorate the circulation of blood, relieving blood stagnate, subsidence of a swelling, stop bleeding and relieving pain caused by injuries from falls, fractures, contusions, strains and traumatic injuries. For quality control, TLC of the extracts of the medicine 1) for Cnidium monnieri L. Cuss and Doubleteeth Pubescent Angelica root, on silica gel with petroleum ether (30-60 ºC) – ethyl acetate 9:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones were visible, viewing under UV 366 nm; 2) for Ligusticum wallichii and Rheum officinale on silica gel with petroleum ether (30-60 ºC) – ethyl acetate 9:1, detection under UV 254 nm; 3) for Oleum Ocimi gratissimi on silica gel with cyclohexane – ethyl acetate 4:1, detection by spraying with 10 % ferric chloride in ethanol and heating at 105 °C until the zones were visible; 4) for Oleum Anisi stellati on silica gel with petroleum ether (60-90 ºC) – ethyl acetate 19:1, detection by spraying with 5 % vanillin in ethanol and heating at 105 °C until the zones were visible; 5) for Oleum Eucalypti on silica gel with cyclohexane – diethyl ether - ethyl acetate 16:3:1, detection by spraying with 5 % vanillin in ethanol and heating at 105 °C until the zones were visible.

Jiangxi J. of Trad. Chinese Med. 43 (350), 59-61 (2012). Fufang Sanqidan capsules are a herbal TCM preparation for treating frequent urination, cardiovascular and cerebrovascular diseases, nephropathy etc. For quality control TLC on silica gel for 1) Radix Notoginseng and Radix Astragali, with the upper phase of n-butanol – ethyl acetate – 10 % ammonia 4:1:5, 2) for Salvia miltiorrhiza, with toluene – ethyl acetate 19:1, detection by viewing in daylight; and 3) for Mulberry Fruit, with petroleum ether (30-60 °C) – chloroform – ethyl acetate 3:8:5. All were detectied by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C for 5 min, viewing under UV 366 nm. Quantification of astragaloside A by HPLC.