St. Hil. Pharmazie 62, 876-880 (2007). TLC of matessaponin 1 and matessaponin 2 on silica gel with chloroform - ethanol - water 12:8:1 and 8:8:1. Detection by spraying with anisaldehyde sulfuric acid reagent followed by heating at 100 °C.

J. Planar Chromatogr. 21, 89-96 (2008). TLC of dyes in normal-phase systems on silica gel, diol phase, cyano phase, and amino phase, and in reversed-phase systems on cyano phase, Diol phase, amino phase, and RP-18. RP chromatography with different mobile phase modifiers (THF, dioxan, methanol, acetonitrile, and acetone) at different concentrations, containing different amines, cationic and anionic ion-pair reagents, buffers, and ammonia, again at different concentrations. Based on the results the best system was selected: HPTLC of tartrazine, sunset yellow FCF, allura red AC, ponceau 4R, brilliant blue FCF, indigotine, brilliant black PN, quinoline yellow, patent blue V, brilliant green BS, azorubin, and brown HT on RP-18 with acetate buffer pH 3.5 containing 15-25 % modifier and 0.025 M propylamine or diethylamine. Detection in white light and under UV 254 and 366 nm. Quantitation by diode array densitometry in the range of 191 to 1033 nm.

Pharmazie 63, 453-458 (2008). Comparison of the Rf value of granisetron hydrochloride alone with the Rf value of granisetron hydrochloride of different drug excipient systems, e. g. beta-cyclodextrin, 2-hydroxypropyl-beta-cyclodextrin, mannitol, and magnesium stearate. No significant change in the Rf value with the different excipients was observed. After TLC separation of compounds, plates were placed into an iodine chamber to identify the spots.

J. Planar Chromatogr. 21, 21-26 (2008). HPTLC of extracts of Hoodia gordonii with fructose and beta-sitosterol as standards on silica gel with chloroform - methanol - water 70:30:3 in an automatic developing chamber fitted with a twin-trough chamber. The chamber was saturated for 20 min with mobile phase and relative humidity was controlled (33 %). Detection by dipping in anisaldehyde reagent, followed by heating at 100 °C for 3 min. Documentation and evaluation before derivatization under UV 366 nm and after derivatization under white light. The method was validated. It is specific and allows discrimination of Hoodia gordonii from Hoodia currorii, Hoodia parviflora, and the common adulterant prickly pear cactus (Opuntia ficus-indica). The sample is stable in solution and on the plate for at least 3 h, as well as during chromatography (2D test). After derivatization the chromatogram is stable for at least 1 hour. Precision (repeatability, intermediate precision, and reproducibility was good and the method is robust . The method is sensitive to changes in relative humidity. If relative humidity exceeds 47% the plate must be conditioned to 33% RH to ensure proper separation.

60th Indian Pharmaceutical Congress PA-206, (2008). HPTLC of (-)hydroxy citric acid in fruits of Garcina Gummigutta on silica gel with n-propanol - water - acetic acid 50:50:1 in a twin-trough chamber saturated for 10 min. Quantitative determination by absorbance measurement at 210 nm. The method was linear in the range of 100-1000 ng/spot. Recovery was 99.8-100.9 %.

J. Liq. Chromatogr. Relat. Technol. 30, 2245-2252 (2007). TLC of authentic vitamin B12 and extract on silica gel with 2-propanol - 28 % ammonia - water 7:1:2 and 1-butanol - 2-propanol - water 10:7:10 in the dark at room temperature. After drying agar containing basal medium and pre-cultured E. coli 215 was overlaid and then incubated at 30 °C for 20 h. After spraying with a methanolic solution of 2,3,5-triphenyltetrazolium salt corrinoid compounds were detected as red zones under white light.

J. AOAC Int. 91, 750-755 (2008). HPTLC of acetylsalicylic acid and clopidogrel bisulfate ((alphaS)-alpha-(chlorophenyl)-6,7-dihydrothieno[3,2-c]pyridine-5-(4H)-acetic acid methyl ester) on silica gel using ethyl acetate - methanol - toluene - acetic acid 50:10:40:1. Quantitative determination by absorbance measurement at 235 nm.

Anal. Bioanal. Chem. 389, 827–834 (2007). A total extract of hen egg yolk is used as phospolipid mixture to demonstrate the capabilities. TLC of phosphatidylcholine, lysophosphatidylcholine, sphingomyelin, phosphatidylethanolamine, lysophosphatidylethanolamine and phosphatidylinositol on silica gel with chloroform - ethanol - water - triethylamine 5:5:1:5. Detection under UV 366 nm after spraying with primuline (Direct Yellow 59) reagent. Direct coupling MALDI-TOF MS and TLC can be easily implemented on commercially available MALDI-TOF devices. Roughly clean spectra without major contributions from fragmentation products and matrix peaks were obtained. This approach was sensitive enough to detect the presence of phospholipids at levels of less than 1 % of the total extract.